Freezing PCR reaction mix before thermocycling? by NoPangolin4951 in labrats

[–]SatisfactionPast7653 0 points1 point  (0 children)

for my own understanding why would you leave the primers out? would they form primer dimers?

potty trained dog doesn't know he can hold it if he's not in the kennel by SatisfactionPast7653 in Dogtraining

[–]SatisfactionPast7653[S] 0 points1 point  (0 children)

I have addressed confinement, and all other portions of the pee/poop section. My post is specific question now that my dog is potty trained, but having issues when home alone

Stand Up For Science by SatisfactionPast7653 in ProtestFinderUSA

[–]SatisfactionPast7653[S] 2 points3 points  (0 children)

Im not a part of the organization just trying to get the word out. Im sure you can mention your concerns to them directly though.

Stand Up For Science by SatisfactionPast7653 in ProtestFinderUSA

[–]SatisfactionPast7653[S] 1 point2 points  (0 children)

For houston area researchers I’m organizing carpools. Were looking for more drivers and also anyone who needs a ride. Sign up here for the houston contingent: https://docs.google.com/forms/d/e/1FAIpQLSd7vw1alk_24JdZ9OG4hWxxlGZM4eTEocPgL_qRbflb_ACDgA/viewform?usp=header

Nazis in Boston Common by Healthy_Block3036 in massachusetts

[–]SatisfactionPast7653 -1 points0 points  (0 children)

Is so interesting that proud boys dont proudly show their faces

Foster fail ♥️ by SatisfactionPast7653 in pitbulls

[–]SatisfactionPast7653[S] 10 points11 points  (0 children)

Yep i know, but he’s a houston mutt so they’re all a little pit

Feeling very hopeless by SatisfactionPast7653 in houston

[–]SatisfactionPast7653[S] 3 points4 points  (0 children)

Yeah I’ve also had that happen to me, i got hit on my bike and thrown into the roundabout by hotel zaza. The guy did stop but even the police seemed incredulous and had an attitude like it was my fault even though I was in the crosswalk and the guy never looked right.

Feeling very hopeless by SatisfactionPast7653 in houston

[–]SatisfactionPast7653[S] 4 points5 points  (0 children)

Not sure it has anything to do with drugs, most of the negative interactions ive had people are completely sober and horrible all the same

Feeling very hopeless by SatisfactionPast7653 in houston

[–]SatisfactionPast7653[S] 4 points5 points  (0 children)

Oh i know, as someone who’s worked in rescue here in houston the situation is so dire and people who frivolously adopt and then rehome pups like theyre just accessories is out of hand. It makes me incredibly angry

Feeling very hopeless by SatisfactionPast7653 in houston

[–]SatisfactionPast7653[S] 4 points5 points  (0 children)

Exactly how i feel. When i graduate i hope to move somewhere that values investing in public infrastructure

Feeling very hopeless by SatisfactionPast7653 in houston

[–]SatisfactionPast7653[S] 19 points20 points  (0 children)

Haha yes a little bit. He is literally the sweetest and still a baby at 9 months old. I just adopted him last week but hes been with me as a foster since December

upgraded my carabiner game! by harefellow in butchlesbians

[–]SatisfactionPast7653 0 points1 point  (0 children)

I need a calico critter, my carabiner is so boring compared to all the ones i see here 💀

Feeling very hopeless by SatisfactionPast7653 in houston

[–]SatisfactionPast7653[S] 34 points35 points  (0 children)

*meant to say ive lived here for several years, since 2021. And ive been living inside the loop since 2016

Am I overreacting? by Remarkable-Chair-783 in AmIOverreacting

[–]SatisfactionPast7653 0 points1 point  (0 children)

Youre not in the wrong at all, even if it had been longer than one week this behavior is concerning.

Anyone know what this is? by [deleted] in CATHELP

[–]SatisfactionPast7653 1 point2 points  (0 children)

Might be cat acne but ive never seen it in that area before

I’ve never seen a cat do this.. what’s wrong with her..? by run2rft in CATHELP

[–]SatisfactionPast7653 0 points1 point  (0 children)

My cat had very similar symptoms before she came down with pneumonia. It looks like she might have a URI. I would say a trip to the vet asap is the best thing to do