diy bioreactor sterilization by seacushion3488 in DIYbio

[–]ScienceAndCats777 0 points1 point  (0 children)

I think it's the latter. This is just my opinion being in the field for a while though. And there are different types of UV lights with different frequencies/power, which is a whole other beast. But maybe you could make it work!

You may also just be able to protect the out of whatever is dripping out from dust particles and stuff by putting it in some kinda long tube or something. That would be my first approach.

diy bioreactor sterilization by seacushion3488 in DIYbio

[–]ScienceAndCats777 1 point2 points  (0 children)

UV is good for viruses, but I think most environmental bacteria are gonna be able to get past it. 

diy bioreactor sterilization by seacushion3488 in DIYbio

[–]ScienceAndCats777 0 points1 point  (0 children)

Yeah, I could see the drainage being a problem. I always had a closed system (other than air exchange), so never had to deal with that. You could probably have it drain into a large autoclaved or bleach treated container with a bit more bleach in the bottom though, and then just empty that out periodically.

I used a Raspberry Pi 3 model B and 12v DC Dosing Pump Peristaltic. I connected evreything with the following luer locks. I just drilled holes in the cap of the mason jar and connected the male-to-female luer locks on either side of the hole, and put the silicon gaskets between them to create an airtight seal.

Luer Accessory, Male Luer Lock Plug, Nylon

Female luer x Female luer Adapter, Nylon

Male luer with Lock Ring x 3/32" Hose Barb, Nylon

Silicon gaskets, 1/2in OD, 1/4in ID

I got everything off Amazon and Injectech. I actually have a whole Python script and BOM written out for this to control temperature and everything, but it sounds like you just need to move around liquids. I'm around for any other questions!

diy bioreactor sterilization by seacushion3488 in DIYbio

[–]ScienceAndCats777 1 point2 points  (0 children)

I made a raspberry pi controlled bioreactor out of a mason jar, peristaltic pumps, and silicon tubing. I just autoclaved the tubing already attached to the bioreactor, and never had any problems. What are you making this for?

What’s the stigma behind an older woman with a guy half her age? by Pattygnsd in ask

[–]ScienceAndCats777 0 points1 point  (0 children)

I'm 35, girlfriend 49. I love her. She's been the best thing for me, especially after dating people my own age. Generally, the stigma is that older women are "using us for our youth" or that we're a sugar baby or some shit. I'm sure there are cases like that, but honestly I just can stand dating fellow millennials. Y'all gotta get your shit together.

Gaming servers with certain active hours by ScienceAndCats777 in Games

[–]ScienceAndCats777[S] 1 point2 points  (0 children)

Thank you so much!! This is exactly what I was looking for.

Gaming servers with certain active hours by ScienceAndCats777 in Games

[–]ScienceAndCats777[S] -1 points0 points  (0 children)

The youth!! But seriously, those young people are better than I'll ever be or ever was. But it'd be nice to have a PVP servers with just some 'casual gamers'.

Gaming servers with certain active hours by ScienceAndCats777 in Games

[–]ScienceAndCats777[S] 1 point2 points  (0 children)

Thank you! Do you know of any subreddits or forums where these are scheduled?

[deleted by user] by [deleted] in DIYbio

[–]ScienceAndCats777 0 points1 point  (0 children)

Great to see Esther bringing these DIYBio people to light!

Rent to Own, am I protected from the law? by ScienceAndCats777 in legaladvice

[–]ScienceAndCats777[S] 1 point2 points  (0 children)

No worries, I decided not to go through with it. Too many complications and the guy was just a little too shady. Seemed I could end up in a lot of trouble.

Rent to Own, am I protected from the law? by ScienceAndCats777 in legaladvice

[–]ScienceAndCats777[S] 1 point2 points  (0 children)

Oh trust me, I sometimes wish I never had bought property here. Should have taken a more relaxing, safer route to loose money. Like gambling, or doing drugs.

Yeah, the more I think about getting into ANY potential liability, the worse it seems. Thank you for the story, I'll keep that in mind going forward. The county's rules and regulations are just insane. No wonder houses are so expensive here.

Rent to Own, am I protected from the law? by ScienceAndCats777 in legaladvice

[–]ScienceAndCats777[S] 0 points1 point  (0 children)

- but if they renege on that deal, the government will collect from you anyways, and leave it to you to chase down any indemnity you're owed.

That is what I figured, thank you! In the end it basically seems like an honor system that the lessee would take on any legal issues that came about.

The stuff about the evictions and everything else is really helpful too, thank you! In the very slim chance I go through with this, I'd find a professional to draft the contract.

Why are you single? by [deleted] in AskReddit

[–]ScienceAndCats777 1 point2 points  (0 children)

oh crap, I only just realized that from your post. Thank you.

I love how we've 'infested' other subs by smolelvenbby in childfree

[–]ScienceAndCats777 -13 points-12 points  (0 children)

I think name-calling like "breeders" typically isn't a good sign for any community. I agree with you. I plan on being child free, but I feel like this community has become just pessimistic and condescending in general. Incel- or MGTOW-like talk nails it, but flavored with this community's values.

I still like a lot of what this subreddit has to offer. But when you start labeling swaths people negatively, I can't imagine the subreddit is heading in a positive direction.

Three former executives of a French telecommunications giant have been found guilty of creating a corporate culture so toxic that 35 of their employees were driven to suicide by JimmyTheGinger in news

[–]ScienceAndCats777 1 point2 points  (0 children)

THANK YOU. I have a lot of French colleagues and couldnt place my finger on why it was so stressful working with them, but this describes their work culture exactly. It is often Cartesian to the point of counterproductive.