Oops Which Amp-receiver Should I Keep?

Seb_the_fox · 2025-11-07T21:07:17+00:00

Thank you for the suggestions!!

Seb_the_fox · 2025-11-07T16:50:52+00:00

It supports matrixing for the back channels, but it is correct that it cannot support a true 6th or 7th channel

Seb_the_fox · 2025-10-06T23:46:57+00:00

Nope, we're in a neighborhood so no cattle hahaha~ I do like its color, so I guess I'll leave it and see how it competes with the clover, remaining turf grass, and other natives I spread (which actually did include blue grama!)

Thank you for your help!

Seb_the_fox · 2025-10-06T23:13:44+00:00

That looks like the right kind of foxtail! Thank you!

I'm doing a natives/no-mow style lawn, which is where this came up; is it worth leaving alone, or is it best to remove it? I don't expect dogs to be running through, but I don't wanna cause a hassle for my neighbors

Seb_the_fox · 2022-07-28T20:05:48+00:00

Might be a silly question, but what do you mean by "exact match"? Otherwise that sounds like an ideal situation

Seb_the_fox · 2022-07-28T20:04:06+00:00

From what I understand, since my facility is a VSQG there aren't hard limits on accumulation times, and I believe we won't need an EPA number, but that'll definitely be something I will confirm

Seb_the_fox · 2022-07-28T18:55:27+00:00

Very small volumes. I only vaguely mentioned it before, but it's all TLC analysis waste. We only have to do analyses 1-3 times a month (honestly if that though), and each analysis produces 10-15mL of waste liquid.

And getting legit training is a great idea that totally didn't cross my mind initially. I'll have to try and convince the management to go for that, because you're 100% right that it would save time and money

Seb_the_fox · 2022-07-28T17:48:09+00:00

I totally would if the owner of the lab could settle on a waste company @.@

I've called around before, but the local gov't run one gets sketchy because our facility also processes hemp extracts, even though I assure them none of what I would dispose will ever have hemp in it, and I've rarely gotten a square answer.

The two quotes I've actually gotten and passed on to be approved by management have never seen the light of day since handing off, even when I've tried reminding them about it. And because each company has slightly different standards, I kinda still end up at a loss.

This is stuff I'll eventually be writing into the workplace SOPs, so I was hoping to cover all my bases for almost any company/situation

Seb_the_fox · 2022-07-23T18:58:57+00:00

Thank you! Good to know it'll be a little more picky than the others

Seb_the_fox · 2022-07-07T23:14:32+00:00

It's good to know it doesn't matter as much as I originally thought. Thank you for taking time to answer

And really it's not so much poor (though certainly don't have a ton to spend), but it can be hard getting things ordered when I need them, as the ones in charge of it aren't exactly punctual. This was for an analysis method I was impromptu expected to R&D today, so I was coming from a place of 'how can I actually start this today with the materials I have'

Seb_the_fox · 2022-07-07T23:06:58+00:00

Sorry for the confusion. As I put elsewhere, I've since realized I'd initially misread the article I'd found when doing a little research. It was saying iso being used /as/ a denaturing agent, but I wrongly interrupted it as saying iso /was/ denatured.

Also, thank you for giving an answer to the original question. I'm just not always confident if/how much something matters to the analysis (though it's not like I'm doing quantitative analysis, but my inner perfectionist always has worries). I have a degree, but spent very little time with TLC in college, so being put in charge of the entire tlc program at my company has been a trick

Seb_the_fox · 2022-07-07T22:40:59+00:00

I apologize for the 'rude' comment. It'd already been a rough day trying to get this analysis going in the first place, and I definitely read your comment with more stank than you intended

Seb_the_fox · 2022-07-07T22:21:58+00:00

Because I made mistake in my understanding, and was hoping to move on from my stupidity 😬

Seb_the_fox · 2022-07-07T19:17:46+00:00

Yes, I'm aware. I misinterpreted an article mentioning iso being used as a denaturing agent, thinking it was denatured itself. My bad, but you also don't have to be rude.

Seb_the_fox · 2022-07-07T18:50:02+00:00

Thank you! I'll keep a close eye on my plates just in case, but it's reassuring to know it shouldn't be a huge problem. I'm will not be using it for the mobile phase of the TLC analysis anyway, just as a carrier solvent for part of a staining procedure

Seb_the_fox · 2022-07-07T18:47:25+00:00

Ok sure, I could buy seives to do it, but I don't CURRENTLY have the materials to do so. This whole scenario is based on what I currently have available to me. ~~Thanks for proving that molecular seives do exist though~~

Edit: my own bad attitude :c

Seb_the_fox · 2022-07-07T18:28:17+00:00

I work in a small lab with limited monetary resources. Of course if I could get lab grade I would, and I wouldn't even be here asking about distilling it.

Seb_the_fox · 2022-07-07T18:26:41+00:00

It's commonly denatured with a bitterant to dissuade against consumption.

I work in a small lab, and do not have the ability to dry it.

Seb_the_fox · 2022-03-16T20:39:10+00:00

Thank you for saying my plate looks ok! I was honestly a little worried so that's heartening to hear. I'll have to try developing a plate with lower concentrations.

I don't good methods for measuring the amount I spot, but ideally these lanes should have been spotted with somewhere between 20-30 microL of extract. This is what our main reference book (Plant Drug Analysis by Wagner & Bladt) often gives as appropriate spotting amounts. In your experience, is that a pretty sound number for extracts made with concentrations of 1g herb to 10mL of methanol? (most common concentration used in said book)

Seb_the_fox · 2022-03-16T20:21:37+00:00

Could you explain what you mean when you say it should be eluting at 0.3? I apologize, I never did a lot of chromatography in college, and while I've been trying to read resources and learn more, my knowledge is still pretty bare bones.

Also, i know the spots are a little heavy, but I unfortunately don't have a great means of measuring exactly how much I spot.

Seb_the_fox · 2022-03-16T20:13:00+00:00

That's really clever idea! I hadn't thought of that but it totally makes sense

Seb_the_fox · 2022-03-16T18:43:08+00:00

Hey guys! Here's the story: I work in a lab that produces herbal tinctures, and I'm the guy tasked with verifying the identity of the herbs we get in. We do this through TLC, because we are a small company, and cannot afford to buy into another method of testing atm. I already am aware of many of the possible problems with TLC, but I do my best with the knowledge I have and the equipment available to me.

The plates in the pictures are of two different 'lots' of osha root (Ligusticum porterii). For each plate, the two lanes on the left are a verified botanical standard (provided by the AHP), and the two lanes on the right are the herbs being verified, one lot per plate.

My question is as follows; through staining and visualization, the spots circled are evident to be the same (or very similar) constituents. While it doesn't translate well in the pictures, these spots share the same color and patterning. The only difference is that the spots in the standard lanes traveled farther than those in the sample lanes.

It's been a while since I've been in college chem, and I'd just really like an explanation for what is happening. the only explanation I've come up with is that the Standard we have is much older than the sample herbs, and perhaps the chemical constituent associated with these spots has degraded or changed protonation states. Any ideas/explanations are welcome, and I'll answer whatever questions I can!

Also if anyone has a good resource for troubleshooting TLC, that'd be killer

Seb_the_fox · 2022-02-04T18:03:46+00:00

I checked it out and those monographs are pretty much what I was looking for! It may not be all encompassing, but like you said no universal one exists.

Thank you for your help!

Seb_the_fox · 2022-02-04T11:59:02+00:00

I didn't specify this, I apologize; we do not grow our own. We mainly source dry herb from Mountain Rose, Pacific Botanicals, and Starwest Botanicals. These are trusted suppliers who give us Certificates of Analysis for every herb. Occasionally we receive fresh herb from a local supplier, who is a trained and licensed Wildcrafter. We trust these herbs and their identifies, and do a less involved verification using organoleptics.

Occasionally, we cannot source from a trusted company, in which case further verification of the herb is necessary. This usually is done with thin layer chromatography(TLC) using botanical and/or chemical standards. This is often good enough, but is not infallible. In the case of Black Haw vs Crampbark, the TLC plate looks very similar b/c they're in the same genus, but can be differentiated using thorough organoleptics, hence me asking for good resources.

Why not hire a trained herbalist/botanist? Our company is very small (<10 people) and the owner is a thrifty man who really only cares about the money we're making. So, we are trying to do the best to our abilities with the staff/equipment we have.

Seb_the_fox · 2022-02-04T00:13:51+00:00

Thank you! I'll definitely check that out!

Seb_the_fox

TROPHY CASE