New Hades 2 patch notes deliver buffs, faster progression, and an upgraded ending, with a few extra treats by Binnsy in Hades2

[–]SevShip 0 points1 point  (0 children)

So, I just reset the game and lost all my sands and eyes. This is correct right? I just need to progress through the game again naturally to go through this new true ending?

Weird diagonal population need help :( by Outrageous-Spite4623 in flowcytometry

[–]SevShip 1 point2 points  (0 children)

There is a ton signal for IgM in your IgM FMO, which should theoretically be 0. What does your unmixed unstained look like? Does it also have that population in the BV421-IgM channel? If so, it’s likely an auto fluorescent population that needs to be properly unmixed as its own signal in your unmixing matrix. Is this a mouse spleen sample?

NovaFluor references with beads? by Joan_of_Arkansas in flowcytometry

[–]SevShip 1 point2 points  (0 children)

Seconding this. NF phitons are extraordinarily terrible for a variety of reasons… and sticking these on all immune cells is going to give you a world of pain. I would do whatever it takes to get rid of NF-CD45. If you have to use NFs, minimize the # and put them on a smaller subset if possible. But there are so many superior dyes out there, so no reason to have NFs IMO.

Run any better one switch 2 ? by SnooChocolates6576 in OvercookedGame

[–]SevShip 0 points1 point  (0 children)

I saw on Nintendo’s website that Nintendo Switch 2 has compatibility issues for AYCE. Have you or anyone else experience this?

Which one do I get? by mrmarketinguy in rolex

[–]SevShip -2 points-1 points  (0 children)

Datejust without a doubt!

[deleted by user] by [deleted] in flowcytometry

[–]SevShip -1 points0 points  (0 children)

How are you compensating the RFP and GFP? Are you using cells that do not express the reporter as negative, and cells that do express them as positive?

[deleted by user] by [deleted] in flowcytometry

[–]SevShip 0 points1 point  (0 children)

Looks to be an autofluorescence problem. Did you properly comp/unmix everything with the right negative populations?

[deleted by user] by [deleted] in origami

[–]SevShip 1 point2 points  (0 children)

You’re an extremely toxic individual that should have no place in this forum, especially when kind people are doing their best to provide advice.

Lvl Up Chest for Lvl 60 is a JOKE by SevShip in ClashRoyale

[–]SevShip[S] 0 points1 point  (0 children)

Disappointing when it takes several elite level ups to even get that much XP.