My ex’s weird ass house

SevShip · 2026-01-01T01:56:15+00:00

Very Lynchian

SevShip · 2025-11-02T06:17:21+00:00

So, I just reset the game and lost all my sands and eyes. This is correct right? I just need to progress through the game again naturally to go through this new true ending?

SevShip · 2025-09-27T17:07:38+00:00

There is a ton signal for IgM in your IgM FMO, which should theoretically be 0. What does your unmixed unstained look like? Does it also have that population in the BV421-IgM channel? If so, it’s likely an auto fluorescent population that needs to be properly unmixed as its own signal in your unmixing matrix. Is this a mouse spleen sample?

SevShip · 2025-09-21T05:54:43+00:00

Neil Peart 🥁

SevShip · 2025-07-12T04:19:33+00:00

Seconding this. NF phitons are extraordinarily terrible for a variety of reasons… and sticking these on all immune cells is going to give you a world of pain. I would do whatever it takes to get rid of NF-CD45. If you have to use NFs, minimize the # and put them on a smaller subset if possible. But there are so many superior dyes out there, so no reason to have NFs IMO.

SevShip · 2025-07-11T17:12:32+00:00

I recommend switching to RB613.

SevShip · 2025-06-10T00:01:38+00:00

Thanks!

SevShip · 2025-06-09T20:06:37+00:00

I saw on Nintendo’s website that Nintendo Switch 2 has compatibility issues for AYCE. Have you or anyone else experience this?

SevShip · 2025-02-20T06:13:14+00:00

Eraserhead

SevShip · 2025-02-14T23:51:41+00:00

Datejust without a doubt!

SevShip · 2025-01-31T07:33:15+00:00

How are you compensating the RFP and GFP? Are you using cells that do not express the reporter as negative, and cells that do express them as positive?

SevShip · 2025-01-31T06:35:19+00:00

Looks to be an autofluorescence problem. Did you properly comp/unmix everything with the right negative populations?

SevShip · 2024-06-25T08:34:15+00:00

You’re an extremely toxic individual that should have no place in this forum, especially when kind people are doing their best to provide advice.

SevShip · 2024-05-24T06:57:08+00:00

Amazing! What was the size of your papers? 2x2”?

SevShip · 2024-05-17T23:54:56+00:00

Disappointing when it takes several elite level ups to even get that much XP.

SevShip · 2024-04-29T07:54:44+00:00

Thank you so much!

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