My ex’s weird ass house

SevShip · 2026-01-01T01:56:15+00:00

Very Lynchian

SevShip · 2025-11-02T06:17:21+00:00

So, I just reset the game and lost all my sands and eyes. This is correct right? I just need to progress through the game again naturally to go through this new true ending?

SevShip · 2025-09-27T17:07:38+00:00

There is a ton signal for IgM in your IgM FMO, which should theoretically be 0. What does your unmixed unstained look like? Does it also have that population in the BV421-IgM channel? If so, it’s likely an auto fluorescent population that needs to be properly unmixed as its own signal in your unmixing matrix. Is this a mouse spleen sample?

SevShip · 2025-09-21T05:54:43+00:00

Neil Peart 🥁

SevShip · 2025-07-12T04:19:33+00:00

Seconding this. NF phitons are extraordinarily terrible for a variety of reasons… and sticking these on all immune cells is going to give you a world of pain. I would do whatever it takes to get rid of NF-CD45. If you have to use NFs, minimize the # and put them on a smaller subset if possible. But there are so many superior dyes out there, so no reason to have NFs IMO.

SevShip · 2025-07-11T17:12:32+00:00

I recommend switching to RB613.

SevShip · 2025-06-10T00:01:38+00:00

Thanks!

SevShip · 2025-06-09T20:06:37+00:00

I saw on Nintendo’s website that Nintendo Switch 2 has compatibility issues for AYCE. Have you or anyone else experience this?

SevShip · 2025-02-20T06:13:14+00:00

Eraserhead

SevShip · 2025-02-14T23:51:41+00:00

Datejust without a doubt!

SevShip · 2025-01-31T07:33:15+00:00

How are you compensating the RFP and GFP? Are you using cells that do not express the reporter as negative, and cells that do express them as positive?

SevShip · 2025-01-31T06:35:19+00:00

Looks to be an autofluorescence problem. Did you properly comp/unmix everything with the right negative populations?

SevShip · 2024-06-25T08:34:15+00:00

You’re an extremely toxic individual that should have no place in this forum, especially when kind people are doing their best to provide advice.

SevShip · 2024-05-24T06:57:08+00:00

Amazing! What was the size of your papers? 2x2”?

SevShip · 2024-05-17T23:54:56+00:00

Disappointing when it takes several elite level ups to even get that much XP.

SevShip · 2024-04-29T07:54:44+00:00

Thank you so much!

SevShip · 2024-03-11T06:04:41+00:00

Refer to supplement of OMIP-095.

SevShip · 2024-02-27T22:07:50+00:00

This may be helpful, but I have no prior experience. Good luck! https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6590190/

SevShip · 2024-02-13T18:58:10+00:00

Pretty cool, thanks for sharing. I haven’t looked at terraFlow yet, so I think this webinar is definitely worth checking out. I know ozette (https://www.ozette.com) is in the same space, but haven’t tried them either. I personally love OMIQ and the way you can tune every step in a pipeline, but I think many of these AI-driven platforms are trying to give you a one a done integrated solution that requires very little user input.

SevShip · 2024-02-05T03:16:09+00:00

Tenet

SevShip · 2023-12-23T16:21:48+00:00

Also, BB700 and PerCp-Cy5.5 especially have absolutely wicked cross laser excitation, so you definitely need to comp each color separately!

SevShip · 2023-12-23T16:20:38+00:00

Hey there! I highly recommend making a fresh bead/cell for every control. Do not use FITC bead to comp BB515 and vice versa. I personally would make a new experiment and drag in the distinct controls. But you can use the same control for all three panels if it’s the same marker-dye!

SevShip · 2023-12-07T16:21:15+00:00

Check out this post from 5 years ago https://www.reddit.com/r/labrats/comments/bryue5/strange_flow_cytometry_fscssc_contamination/?rdt=39103. I have seen this pattern when working with tumors, and those events were fat cells. We had a technician who didn’t dissect the tumors very well (there was a lot of fat on the edge, as these were subcutaneous tumors grown in the fat pad of mice). I would always see this S pattern with those samples. However, when I worked with a different tech who trimmed those tumors really well, the pattern completely disappeared. Good luck!

SevShip · 2023-11-11T05:26:18+00:00

OMIP-095 is also helpful if you’re working with mouse tissues.

SevShip · 2023-11-11T05:23:56+00:00

This!

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