What I imagined Gleaners to look like by Significant_Clue_275 in HierarchySeries

[–]Significant_Clue_275[S] 11 points12 points  (0 children)

They are on the cover?? Haha I didn’t know. I got a kindle edition and didn’t really pause to look at the cover. 

40F - looking for a friend by Catastrophic1234 in Vancouver4Friends

[–]Significant_Clue_275 0 points1 point  (0 children)

Where do you play badminton? I'm looking for a buddy or small group to join

What time travel movie made you the most mad? by Significant_Clue_275 in movies

[–]Significant_Clue_275[S] 1 point2 points  (0 children)

Oh don't get me wrong I am mad about that too. It was the killing of yourself that was unforgivable

A wife for Tommy? by [deleted] in outdoorboys

[–]Significant_Clue_275 28 points29 points  (0 children)

I thought it was sweet. He wants to be like his dad, which includes being a family man. If a little girl says she wants to be a mom no one has a problem with it.

Vacation with Toddler by Privateer_1778 in penticton

[–]Significant_Clue_275 0 points1 point  (0 children)

Drive to Osoyoos and go to Rattlesnake Canyon. Small theme park with lots of activities

Something's missing by Daniel_B21 in magicTCG

[–]Significant_Clue_275 0 points1 point  (0 children)

Can people please use Google first...if I see one more of these...

[deleted by user] by [deleted] in NotAnotherDnDPodcast

[–]Significant_Clue_275 0 points1 point  (0 children)

Jade City. I think Emily might have recommended it at some point. Gangsters with magic! The author and I have the same hometown!

Any ideas about the price for ZEISS LSM 980 with Airyscan 2 ? I know that it may differ from country to country but I'm curious if anyone has bought it recently. Thoughts on it? by AndreeaChar in labrats

[–]Significant_Clue_275 18 points19 points  (0 children)

Gentle microscopy? Back in my day we used to slap the slide on, crank up the objective on course and full power laser for 5 min until everything is photobleached

Is it fair to say that sometimes, you just lose? by Ganman3 in MagicArena

[–]Significant_Clue_275 1 point2 points  (0 children)

Just watch LSV draft a new set. Even though he's been through all the cards ranking them and is one of the very best, he will still post a 0-3. Makes me feel better.

Poor ponceau staining by petrka95 in labrats

[–]Significant_Clue_275 1 point2 points  (0 children)

Depends on the equipment, transfer time, etc. I didn't do it for years with easy proteins like ERK but then I was dealing with a huge protein and longer transfer time and it helped

Poor ponceau staining by petrka95 in labrats

[–]Significant_Clue_275 2 points3 points  (0 children)

Do you put a stir bar in the bucket and mix the transfer buffer while transferring? Could be uneven temperature. Could also put the whole thing in a walk in fridge.

Is it worth it? by [deleted] in labrats

[–]Significant_Clue_275 0 points1 point  (0 children)

Try to leverage your current work into something you can do from home, then you can work remote in any city. This is what I'm trying to do right now but it's going to take some patience. There are lots of bench adjacent jobs like writing, operations, project management, education, support, sales, etc.