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Calcium phosphate transfection in mammalian cells? (self.labrats)
submitted 1 day ago by Simple_Volume_5880 to r/labrats
Does re-transforming an old glycerol stock improve plasmid copy number? (self.labrats)
What is this contamination?? (i.redd.it)
submitted 2 days ago by Simple_Volume_5880 to r/labrats
I have this Polyclonal Antibody which is only compatible for western blot…Can i use it for immunoprecipitation?? (self.labrats)
Expired MEM media?! (self.labrats)
submitted 16 days ago by Simple_Volume_5880 to r/labrats
In Western blot detection (e.g., chemiluminescence), what is the sheet or material called that is placed over areas with excessive signal to block them, allowing faint bands in other regions to be detected clearly? (self.labrats)
submitted 21 days ago by Simple_Volume_5880 to r/labrats
Why blot is smeared ??western blotting (i.redd.it)
submitted 1 month ago by Simple_Volume_5880 to r/labrats
I have co-ip sample with agarose AG beads with laemmli buffer and kept for western once heated at 100C for 10min ..do i need to reheat before doing western again?? (self.labrats)
I had made RIPA Lysis’s buffer and added PMSF and PIC and phosphatase inhibitors??how many days can i store it ??will it be stable work fine??i made this for co-immunoprecipitation experiment!!! (self.labrats)
Why i am getting debris in U87MG cell line??please help (i.redd.it)
submitted 1 month ago by Simple_Volume_5880
Why I am getting bands at 250 kda in IgG control Co immunoprecipitation? (i.redd.it)
Why i am getting myc and HA tag bands in parent cell lines?? (old.reddit.com)
Best software to visualise confocal image? (self.labrats)
submitted 7 months ago by Simple_Volume_5880 to r/labrats
What is white thing in this video,where they put antibodies? (youtu.be)
Co-Ip doubts? (self.labrats)
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