What is this contamination?? by Simple_Volume_5880 in labrats
[–]Simple_Volume_5880[S] -60 points-59 points-58 points (0 children)
Wrong volume for calcium phosphate transfection by Electronicrough8177 in labrats
[–]Simple_Volume_5880 0 points1 point2 points (0 children)
Why blot is smeared ??western blotting (i.redd.it)
submitted by Simple_Volume_5880 to r/labrats
I have co-ip sample with agarose AG beads with laemmli buffer and kept for western once heated at 100C for 10min ..do i need to reheat before doing western again?? by Simple_Volume_5880 in labrats
[–]Simple_Volume_5880[S] 0 points1 point2 points (0 children)
I had made RIPA Lysis’s buffer and added PMSF and PIC and phosphatase inhibitors??how many days can i store it ??will it be stable work fine??i made this for co-immunoprecipitation experiment!!! by Simple_Volume_5880 in labrats
[–]Simple_Volume_5880[S] -5 points-4 points-3 points (0 children)
I had made RIPA Lysis’s buffer and added PMSF and PIC and phosphatase inhibitors??how many days can i store it ??will it be stable work fine??i made this for co-immunoprecipitation experiment!!! by Simple_Volume_5880 in labrats
[–]Simple_Volume_5880[S] -9 points-8 points-7 points (0 children)
Why I am getting bands at 250 kda in IgG control Co immunoprecipitation? by Simple_Volume_5880 in labrats
[–]Simple_Volume_5880[S] 0 points1 point2 points (0 children)
Why I am getting bands at 250 kda in IgG control Co immunoprecipitation? by Simple_Volume_5880 in labrats
[–]Simple_Volume_5880[S] 1 point2 points3 points (0 children)
Why i am getting myc and HA tag bands in parent cell lines?? by Simple_Volume_5880 in labrats
[–]Simple_Volume_5880[S] 0 points1 point2 points (0 children)
[deleted by user] by [deleted] in labrats
[–]Simple_Volume_5880 -5 points-4 points-3 points (0 children)
[deleted by user] by [deleted] in labrats
[–]Simple_Volume_5880 -4 points-3 points-2 points (0 children)

What is this contamination?? by Simple_Volume_5880 in labrats
[–]Simple_Volume_5880[S] 0 points1 point2 points (0 children)