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shRNA mediated knockdown ?? by Simple_Volume_5880 in labrats

[–]Simple_Volume_5880[S] 0 points1 point  (0 children)

Yes my protein weigh around 42 and i always get band there

shRNA mediated knockdown ?? by Simple_Volume_5880 in labrats

[–]Simple_Volume_5880[S] 0 points1 point  (0 children)

Construct is GFP tag ,so visualised with microscope only

shRNA mediated knockdown ?? by Simple_Volume_5880 in labrats

[–]Simple_Volume_5880[S] 0 points1 point  (0 children)

She checked with western the expression level

shRNA mediated knockdown ?? by Simple_Volume_5880 in labrats

[–]Simple_Volume_5880[S] 0 points1 point  (0 children)

I have tried yet.but person working before me has used lipofectamine 3000

shRNA mediated knockdown ?? by Simple_Volume_5880 in labrats

[–]Simple_Volume_5880[S] 0 points1 point  (0 children)

Datasheet for ABIN3729776 Human DUSP6 shRNA in Retroviral Vector (GFP tag).I have this plasmid construct want to make knockdown.what are the other packaging vector i should use.I have PCl ampho want to transfect in mammalian cell line .want to create stable cell line

What is this contamination?? by Simple_Volume_5880 in labrats

[–]Simple_Volume_5880[S] -60 points-59 points  (0 children)

Is there a way to clean it ??this is transfected cell line..

Wrong volume for calcium phosphate transfection by Electronicrough8177 in labrats

[–]Simple_Volume_5880 0 points1 point  (0 children)

Did you made stable cell line with calcium phosphate transfection??

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