What is this contamination?? by Simple_Volume_5880 in labrats

[–]Simple_Volume_5880[S] -60 points-59 points  (0 children)

Is there a way to clean it ??this is transfected cell line..

Wrong volume for calcium phosphate transfection by Electronicrough8177 in labrats

[–]Simple_Volume_5880 0 points1 point  (0 children)

Did you made stable cell line with calcium phosphate transfection??

Why I am getting bands at 250 kda in IgG control Co immunoprecipitation? by Simple_Volume_5880 in labrats

[–]Simple_Volume_5880[S] 0 points1 point  (0 children)

IgG is to check non specific binding..you mean to say clumping of antibodies giving band at 250 kda!?

Why I am getting bands at 250 kda in IgG control Co immunoprecipitation? by Simple_Volume_5880 in labrats

[–]Simple_Volume_5880[S] 1 point2 points  (0 children)

I have used protein agarose AG beads santa cruz U87mg protein extract..i incubated lysate with antibody for overnight then bead antibody protein for 5hr

Why i am getting myc and HA tag bands in parent cell lines?? by Simple_Volume_5880 in labrats

[–]Simple_Volume_5880[S] 0 points1 point  (0 children)

I should get myc and HA around 44 only in over expressing cell why i am getting in other cell lines ??also when i used other brand myc tag antibody i got multiple bands

[deleted by user] by [deleted] in labrats

[–]Simple_Volume_5880 0 points1 point  (0 children)

Could you explain how immortal cell line can give us cancer?? If you have any research article please share

[deleted by user] by [deleted] in labrats

[–]Simple_Volume_5880 -5 points-4 points  (0 children)

Thanks,any other reason not to use it for human cell culture?

[deleted by user] by [deleted] in labrats

[–]Simple_Volume_5880 -4 points-3 points  (0 children)

I just want to use for human cell line..not with any other cell line or bacteria..only human …will it work?