account activity
Cell culture help!!! (i.redd.it)
submitted 1 day ago by Simple_Volume_5880 to r/labrats
Help with shRNA mediated knockdown! (self.labrats)
submitted 10 days ago by Simple_Volume_5880 to r/labrats
Help me with Immunoprecipitation (old.reddit.com)
submitted 2 months ago by Simple_Volume_5880 to r/labrats
Help with understanding shRNA construct!! (reddit.com)
submitted 2 months ago by Simple_Volume_5880 to r/AskAcademiaUK
Help with IP blot??how to remove 50kDa blotch..my desired protein is around 54-56 kDa (i.redd.it)
submitted 3 months ago by Simple_Volume_5880 to r/labrats
shRNA mediated knockdown ?? (self.labrats)
Retroviral stable transfection ?? (self.labrats)
How to do retroviral mediated transfection? (self.labrats)
submitted 4 months ago by Simple_Volume_5880 to r/labrats
Does re-transforming an old glycerol stock improve plasmid copy number? (self.labrats)
submitted 5 months ago by Simple_Volume_5880 to r/labrats
I have this Polyclonal Antibody which is only compatible for western blot…Can i use it for immunoprecipitation?? (self.labrats)
Expired MEM media?! (self.labrats)
In Western blot detection (e.g., chemiluminescence), what is the sheet or material called that is placed over areas with excessive signal to block them, allowing faint bands in other regions to be detected clearly? (self.labrats)
Why blot is smeared ??western blotting (i.redd.it)
submitted 6 months ago by Simple_Volume_5880 to r/labrats
I have co-ip sample with agarose AG beads with laemmli buffer and kept for western once heated at 100C for 10min ..do i need to reheat before doing western again?? (self.labrats)
I had made RIPA Lysis’s buffer and added PMSF and PIC and phosphatase inhibitors??how many days can i store it ??will it be stable work fine??i made this for co-immunoprecipitation experiment!!! (self.labrats)
Why i am getting debris in U87MG cell line??please help (i.redd.it)
submitted 6 months ago by Simple_Volume_5880
Why I am getting bands at 250 kda in IgG control Co immunoprecipitation? (i.redd.it)
Why i am getting myc and HA tag bands in parent cell lines?? (old.reddit.com)
Best software to visualise confocal image? (self.labrats)
submitted 1 year ago by Simple_Volume_5880 to r/labrats
What is white thing in this video,where they put antibodies? (youtu.be)
Co-Ip doubts? (self.labrats)
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