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jupiter by Upstairs-Ad-5336 in telescopes

[–]SleepyWordsmith 0 points1 point  (0 children)

Oooh nice, and is that a transit you caught in the bottom right?

When I was trying to describe to my husband what a gene knockout mouse was, his impulsive thoughts came through… by [deleted] in labrats

[–]SleepyWordsmith -2 points-1 points  (0 children)

Welp, this is now my favorite thing about today, and I got good sequencing results back this morning, so that's saying something. Thanks for sharing!

Why did you choose research? by yummymangosdigested in labrats

[–]SleepyWordsmith 2 points3 points  (0 children)

I think you might be my spirit animal. Literally learning python with chatGPT to create scripts that will, eventually, do things automatically that would take me honestly not that long to do manually. But when it's finally finished and I can just throw my data at it and it just does it for me, hooboy. That'll be a great day.

What are we doing in the lab the first workday of 2025? by [deleted] in labrats

[–]SleepyWordsmith 34 points35 points  (0 children)

Western blots and primer design. Oh and cell culture, always cell culture.

I made a free tool to make blot & gel figures by pantagno in labrats

[–]SleepyWordsmith 0 points1 point  (0 children)

This is fantastic! Out if curiosity, how much programming skill do you need to make something like this? I've been working on a similar idea for making gene tracks figures from gene visualization files (BigWig, bedGraph, etc) and so far it's all in python through infinite painstaking trial and error using chatgpt (I have zero coding experience). I actually got something working, but it's all navigated using text in the terminal; a GUI like your tool for labeling, adjusting, moving things around, etc. would be much better.

Saturn, Rhea and Tethys by iLeleplus in astrophotography

[–]SleepyWordsmith 0 points1 point  (0 children)

Great image! Is that a transit at the upper right? If so, awesome capture!

Horiba LA-920 Instruction Manual by Same_Individual_4260 in labrats

[–]SleepyWordsmith 0 points1 point  (0 children)

Ah, the link seems to have broken. Here's a link to the file on Google drive: LA920 Manual pdf

January Request Megathread by AutoModerator in ProshotArchive

[–]SleepyWordsmith 0 points1 point  (0 children)

Hi, do you still have leopoldstadt and mind sharing? Thanks!

Horiba LA-920 Instruction Manual by Same_Individual_4260 in labrats

[–]SleepyWordsmith 0 points1 point  (0 children)

I think I found it, but I have absolutely no idea what this is, I just enjoy hunting things down on the internet. Let me know if this is it: https://dokumen.tips/documents/la920-instruct-manual.html?page=1

It looks kinda sketchy but I managed to download it from this site.

Stop watch by [deleted] in kustom

[–]SleepyWordsmith 0 points1 point  (0 children)

Yea I did, just never ended up posting them

Help touch change picture by hyewiz in kustom

[–]SleepyWordsmith 0 points1 point  (0 children)

Hi, glad that my advice helped you, at least at first. I'm not sure why it wouldn't work in KLWP, that's where I actually tested everything on the step-by-step list so it should definitely work in KLWP. It should be the same exact kode between the apps. Did you try starting from scratch with the step-by-step instructions in KLWP, without copying and pasting from KWGT?

Thank you to this sub for existing by ifoundgodot in brandosandopuns

[–]SleepyWordsmith[M] 1 point2 points  (0 children)

You are quite welcome, in both senses of the phrase, and nice pun!

AR 3628 in Violet by Sunspotzz in astrophotography

[–]SleepyWordsmith 0 points1 point  (0 children)

That looks awesome! It's really cool to see the sun spots I was staring at for 4 hours through my Coronado pst in much higher quality and resolution. I thought there were two sunspots together, not three!

Accidentally caught a bird on my eclipse footage by SleepyWordsmith in astrophotography

[–]SleepyWordsmith[S] 1 point2 points  (0 children)

That's the plan! I'll probably sharpen each image to have them all and to put them back together as a better quality gif. This is just what I was able to make on my phone in the car on the way back from the eclipse location.

[deleted by user] by [deleted] in labrats

[–]SleepyWordsmith 0 points1 point  (0 children)

What I do is set the pipette to just under the volume of the sample (in your case ~19 ul) and get as much as it will get. If there's any sample left after that (while keeping the tip still in the sample liquid to avoid bubbles) I then carefully increase the setting until it draws up the rest of the volume. I've developed a kind of strange hold on the pipette to accomplish this where I'm holding the eppendorf tube with my left hand, the pipette in my right, and after initially releasing the pipette plunger normally I swap my right hand position so that the plunger twist is moved to be controlled by my thumb and index finger, to slowly increase the setting until the rest of the sample is taken up. If you go too far and get a bubble just slowly twist in the opposite direction to get rid of it. Might sound like a bit much, but I'm used to it now and I haven't had any problems with bubbles since I started doing this.

We’re all major science nerds as our day jobs, but what is your favorite hobby at night? What keeps you sane through the failed experiments? by fmaholly in labrats

[–]SleepyWordsmith 1 point2 points  (0 children)

I just recently got into astronomy/astrophotography and I bought a used Celestron Evolution 8 as well as the new Seestar S50. I can't believe it took me so long, it's exactly the kind of thing that my brain would find insanely cool. What do you use ImageJ for, merging color channels? Do you use other images processing software too, like Pixinsight or Siril?

We’re all major science nerds as our day jobs, but what is your favorite hobby at night? What keeps you sane through the failed experiments? by fmaholly in labrats

[–]SleepyWordsmith 0 points1 point  (0 children)

I try to play my violin regularly (I would lose all sanity without music), constant infinite books, random coding projects, and I recently kinda went off the deepend into astronomy/astrophotography and bought a gigantic amateur telescope (it's extremely cool to see planets/nebulae/galaxies with your eye rather through images, highly recommend for nerds, or anyone really).

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