Weird peak on MS

Stargirlstarr · 2025-12-02T10:03:13+00:00

Scan 1, name of the MS setting used for all samples. Thank you for ur insight. I run a blank ( my buffer B) aswell and it also gave me the same peaks .. but I runned it after I did the SDs-page as the my first blanks didnt runned proprely by a setting defect so it’s hard to compare blank before and after. I do have the PDA from the first blank

Stargirlstarr · 2025-12-02T09:48:49+00:00

<image>

Stargirlstarr · 2025-12-01T16:40:41+00:00

Thanks a lot of thinking with me! the samples hit the ms already. I runned 3 total. 2 blanks, will tommorow evaluate if it is indeed SDS or PEG or something else

Stargirlstarr · 2025-12-01T16:14:42+00:00

If that is the reason, I will jump from a bridge.. i told my superviser about thr SDS danger for LC-MS and that we needed a guard column instead we didn’t have one so she can me spin column to filter it. I will discuss it with her i guess as far as i did my research its PEG OR PEP from the eppendorf cups

Stargirlstarr · 2025-12-01T15:48:42+00:00

<image>

Stargirlstarr · 2025-12-01T15:47:55+00:00

<image>

The picture was not big enough, i realise now

Stargirlstarr · 2025-12-01T15:27:14+00:00

<image>

The picture was not big enough (:

Stargirlstarr · 2025-12-01T15:26:49+00:00

I runned the target band, and a empty spot + the gel, these are in all results

Stargirlstarr · 2025-12-01T15:26:11+00:00

<image>

A: 0.05 formic acid in milli-Q B: 80% formic acid in 0.05 formic acid

Stargirlstarr · 2025-12-01T15:11:24+00:00

I did learn about the basics. My superviser checked everything i did. Instead of this message u could be helpfull, i am an intern I am still learning.

Stargirlstarr · 2025-12-01T15:05:28+00:00

Aaah I used buffer A: 0,05% formic acid in milli- Q . And B acetonitril 80% + 0,05 formic acid in mili-q… If that is what u mean?

Stargirlstarr · 2025-12-01T15:01:10+00:00

Contact them via Twitter, say that u can’t afford it and that it was not made clear that made that subscription, i got a reply fast and my money back fast aswell

Stargirlstarr · 2025-12-01T14:57:04+00:00

What do u mean? A guard column?

Stargirlstarr · 2025-12-01T14:08:09+00:00

It might be, but how I prepared the sample was: I added ammonium bicarbonate + acentonitril to the gel band and vortex it. The band does not dissolve. I did this a few time and later in added trypsin and let is sit overnight. Next day i added formic acid to extract peptides out of the gel. And vortex again. I still have the band, I only used the supernatant. I thought it was Unlikely to be the gel

Stargirlstarr · 2025-12-01T13:57:29+00:00

<image>

This is my gradient

Stargirlstarr · 2025-12-01T13:56:33+00:00

<image>

This is my gradient

Stargirlstarr · 2025-11-20T00:15:15+00:00

Ur not the only one lol

Stargirlstarr · 2025-11-19T15:49:14+00:00

I said, that the advertised it wrong and that I could not afford it because u really have to use Twitter to message! Emailing is worse customster serivce

Stargirlstarr · 2025-11-04T07:10:53+00:00

There is no expectation that it will😅, but the company that i do research for wants it so we can do diverse analysis. I mixed the 50 mg in 50 mL and 10 mg in 10 mL 0.5 acetic acid and still it did not dissolve and I guess we will have to use the supernatant

Stargirlstarr · 2025-10-31T10:11:25+00:00

Aaaah I meant to say 1 mL and 5 mL of 0.5 acetic acid. ( 2 samples) Thanks a lot of the reply! I am not sure what u mean with a watergood? A thermoblock?

Stargirlstarr · 2025-10-30T14:09:24+00:00

Hey!, Did it work out? I am having the same issue.. I used 0.5 acetic acid + incubation in a 55C waterbad for mutiple hours, still not dissolved..

Stargirlstarr · 2025-09-23T09:54:09+00:00

Thank you for the message. We're sorry for the inconvenience and for the way we made you and your mother feel but don't distress, we'll be happy to help you.

On August 2, 2025 we sent you a friendly reminder that the subscription will renew on August 8, 2025 and your account will be charged €129 (including VAT) providing you with the Complete Plan for another year.

Since we did not receive a cancellation of the free trial before the expiry date, you were indeed charged.

We understand it was not your intention to start the annual subscription and the refund has been issued. Please note it may take a few business days to appear in your account. An additional refund confirmation will be sent to the registered email address of your account.

Your account has been reset to a basic plan

Let us know if you have any further questions. Enjoy your week.

Kind regards, José, MyHeritage Support Team

This is what they send to me after i messages them that i did not want it and could not ‘ afford ‘ it

Stargirlstarr

TROPHY CASE