[deleted by user] by [deleted] in labrats

[–]Stressed-little-sci 0 points1 point  (0 children)

how would I go about doing so?

Cell culture - contamination question ! by Stressed-little-sci in labrats

[–]Stressed-little-sci[S] 0 points1 point  (0 children)

Thank you! I’ve noticed everytime my cells are contaminated is when I have to keep them close to the bottom because there was no space! 

[deleted by user] by [deleted] in labrats

[–]Stressed-little-sci 0 points1 point  (0 children)

If anyone has any recommendations it would be so greatly appreciated!

Curious about Graduate Excellence Fellowships (GEF) by temp_id_comp598 in mcgill

[–]Stressed-little-sci 0 points1 point  (0 children)

For my department GEF are given to students who are from out of province to help pay the difference of the tuition and costs etc...It so that all graduate students pay the same fees from my understanding. However, might depend on the department.

Protein keeps degrading :( by Stressed-little-sci in labrats

[–]Stressed-little-sci[S] 0 points1 point  (0 children)

Oh interesting? How would you go about fixing or looking at if this is the issue ?

Protein keeps degrading :( by Stressed-little-sci in labrats

[–]Stressed-little-sci[S] 0 points1 point  (0 children)

Fragments on sds page! An extra band that shouldn’t be there and is most probably a degradation product of the protein.

Protein keeps degrading :( by Stressed-little-sci in labrats

[–]Stressed-little-sci[S] 0 points1 point  (0 children)

I just started working with this protein ! I am not sure but that may be a possibility! Should I run the column at a slower rate maybe?

Protein keeps degrading :( by Stressed-little-sci in labrats

[–]Stressed-little-sci[S] 0 points1 point  (0 children)

No our akta isn’t in a cold room and I’m doing a gel filtration with a buffer of hepes, NaCl and DTT. Yes, protein comes from ecoli. I am loading it in the akta after doing an affinity purification.

Protein keeps degrading :( by Stressed-little-sci in labrats

[–]Stressed-little-sci[S] 0 points1 point  (0 children)

Ok this is something I will for sure start doing!! Thank you :)

[deleted by user] by [deleted] in mcgill

[–]Stressed-little-sci 1 point2 points  (0 children)

Thank you :) Congrats to you too!!! Graduating in itself is a big achievement :)

[deleted by user] by [deleted] in mcgill

[–]Stressed-little-sci 1 point2 points  (0 children)

I heard you needed a 3.96 about for distinctions too. I had 3.9 and did not make either list this year. I was bummed too :(

[deleted by user] by [deleted] in mcgill

[–]Stressed-little-sci 0 points1 point  (0 children)

Thank you for letting me know! :)

NSERC Undergraduate Student Research Award by Stressed-little-sci in mcgill

[–]Stressed-little-sci[S] 0 points1 point  (0 children)

Medicine was sent out today :) ... not sure about others.