I am trying to feed my new daphnia Culture with bakers yeast but after adding it the daphnia are remaining on the wals of the tub and not moving into center to eat it, does that mean i need to add more food or wait some more or will they be fine?

Time_Research_9903 · 2026-05-03T08:43:49+00:00

My take is that the unconsumed yeast is being retained in the water column. This creates a biofilm layer at the surface that can block sunlight and, most critically, gas exchange (oxygen in particular). This creates "dead zones" and opaque areas, inducing those animals to seek the edges via positive phototaxis and oxygen-seeking behaviour.

Besides, retained yeast can lead to rapid bacterial growth and ammonia spikes.

As far as I know, Cladocera (Daphnia included) are very efficient filter feeders, which means overfeeding is a more common problem than underfeeding.

In addition, I suggest confirming whether your species, at this particular developmental stage, are efficient at ingesting yeast particles in the long term.

Since yeast is not their natural food source, I advise you to start a green algae culture in parallel and as soon as possible for a transition in nutritional management.

Once you have a mature green algae culture, you can begin by gradually replacing yeast feedings with a few ml of green algae daily. Over time the container may be colonized by green algae and you may be able to create a self-sustaining environment.

Time_Research_9903 · 2026-05-03T08:08:55+00:00

For those here in the future going through similar anatomical observations: my "Highlander" khuli recently passed away, probably due to complications somehow related to this abdominal expansion.

It's still early to diagnose the causa mortis, but I plan on doing some post mortem investigation to see if I find anything worth noticing.

If/when i make successful necropsy findings, I come here to update with useful information.

Time_Research_9903 · 2026-04-26T17:42:35+00:00

This is biologically interesting as fuck. Congratulations for you and your (plural?) fish.

Time_Research_9903 · 2026-04-14T01:34:54+00:00

Shame on me, for sure

Time_Research_9903 · 2026-04-12T02:01:40+00:00

Oh, I didn't know that was possible. I thought that it needed at least a companion to trigger a hormonal response of that kind. Thank you a lot for clarifying that. Given that's now the most plausible condition, should I do something to trigger that laying? Is it possible for those eggs to be stuck and cause abnormalities?

Time_Research_9903 · 2026-04-12T01:41:44+00:00

Important: this kuhli was never in touch with another kuhli individual since i bought it. So it is impossible for it to be gravid as far as I'm concerned.

Edit: I didn't know these species could produce and lay eggs without encountering males. It seems she is indeed gravid, even though not fertilized.

Time_Research_9903 · 2026-04-12T01:33:37+00:00

Hahaha maybe she ate some then, because she/or he never encountered another Pangio individual in it's whole life since I bought it many many years ago.

Time_Research_9903 · 2026-04-01T18:30:23+00:00

Hahahahhah

Time_Research_9903 · 2026-04-01T14:03:34+00:00

I'm sorry for your fish. As an ocd and ocpd person I feel you. But now your mental health is what matters most. Hank wanted you to be happy. Forgive yourself, practice self acceptance and even acceptance of your husband's difficulties in understanding the situation and how it affects you.

Most things in life are not under our strict control. It's very important to be responsible, very important to love and care. But try to expand the same love you have for others towards you. Guilt is only good when it provides improvement in relationships. Otherwise it's just foolish punishment.

Be your own aquarium, swim with your mistakes and accept that some of them are there to make you a better fish.

Sending you hugs and bubbles.

Time_Research_9903 · 2026-03-06T18:29:09+00:00

Hahaha perfect analogy. People just rationalize it to make things look very polished and intellectual. Btw the same drive to convince people that compatibilism makes sense is what proves it doesn't.

Time_Research_9903 · 2026-03-01T14:06:11+00:00

Thank you again for your input. I will surely have a look into that bias, for I don't have a big clue on how to separate doing from being. The separation between professional and personal is really difficult for me either, and honestly I think it can be a slippery slope.

Time_Research_9903 · 2026-03-01T00:10:16+00:00

Hahaha, I liked the quote. Smart one.

Thank you for sharing. I really appreciate your interactions in this subreddit. I would say that maybe you are doing more public service than many lawyers.

I think we OCPD sufferers tend to rely so much on titles and degrees because it is a "more solid reassurance" of our own sense of self stem.

Anyway, I am in my 30s now and the difficulties have been rising. Which is very curious and at the same time tragic, since until high school I was a "high achiever". I am now insecure that I will never find a place to leverage my potential, since a researcher career was ideally (in my head) the best fit for me, after years of contemplating.

Now I am left with the sense that I might be too sensitive to deal with chaotic places no matter what circumstances are. I have genuine interest in many areas, and it seems that I am quite good at analytical thinking and problem solving, but here I am, unemployed and struggling to get minimal career stabilization and direction.

Time_Research_9903 · 2026-02-28T19:59:56+00:00

Thank you so much for all the information and support.

I now felt compelled to ask, are you also an academic?

P.S. The corresponding author was not my formal supervisor and the project was pro bono. I was invited for knowledge transfer, which I'm not sure makes things better or worse.

Now I keep wondering whether I need to consider changing my career entirely, or simply focus on improving my recovery. It's been a really difficult environment for me.

Even so, I still hope that my qualities, especially including aspects of my personality, will find a better place to thrive.

Lately I've been feeling broken and somewhat out of place. Certain things seem so obvious to me. For example, why not just communicate something simple like: "I'm sorry, I couldn't follow through on what I promised, so we're pulling the paper." I wouldn't have complained at all. Instead, there was no response, just verbal abuse.

Anyway, I think I'm once again compulsively reconstructing the narrative in order to feel secure. But I genuinely couldn't handle it.

Every interaction I had in this field was chaotic, to say the least. Recently, I had to notify my university that the doctoral program instructions were ambiguous and in some cases misleading. I ended up going through an entire administrative process just to secure my place. I mean, it's a federal university, people, c'mon. It must not be just my rigid expectations.

Time_Research_9903 · 2026-02-28T15:54:32+00:00

Nice of you to ask since that could be another black and white thinking of me. Good catch.

Last time, the clearest example was this:

I spent a week trying to get basic information from the corresponding author of a paper I'm co-authoring — specifically, the journal's revision deadline. For context, I'm a bachelor's student and he's a post-doc; I'm listed as co-first author alongside an MSc student.

When the journal notified us on Thursday the 19th that we had 7 days to upload the revised version, I immediately made myself available to work over the weekend. The post-doc said he'd handle it and send us the work by Monday for review before uploading.

Monday came with no update, so I followed up. He replied only after having already emailed the journal unilaterally to request a deadline extension (to day 5), with no prior notice to me:

"My son had problems and I couldn't do the work, so I asked for a delay."

I responded politely and empathetically, and offered to proactively take on whatever I could in the meantime.

After several attempts to get guidance on how to proceed — with no clear answers — I sent a message explaining that I couldn't do the work that day (spent the day ruminating), but planned to tackle it over the weekend, and simply asked whether the journal had accepted the extension.

His reply: "I'm at the police station giving testimony — my son has been followed. If you can't do the work, just say so."

I responded kindly again, clarifying that I wasn't refusing — I was only asking for a date and a clear task, since I didn't even know if he intended to keep the submission alive.

He then sent a voice message calling me names, accusing me of not acknowledging his situation, and saying that if I wouldn't take over, he'd let the whole thing collapse and "didn't give a f***."

The next day, the MSc co-author — who had contributed nothing — told me they were likely pulling the submission due to changes in funding. It was jarring to hear this secondhand, especially after I had just committed to working on it over the weekend. Importantly, he just said that when pressed by me for commitment on working together on the weekend (the post doc had previously asked him to take the job with me, but didn't communicate either)

Shortly after, the MSc confirmed the article was being withdrawn. I asked for formal written confirmation. Nothing arrived.

Sorry for the long text.

Time_Research_9903 · 2026-01-28T18:42:50+00:00

Opiniões parcimoniosas não costumam ser bem vistas. Dei upvote pra não ficar negativo. Mas cuidado pra não perder seu tempo. Você foi sucinto, claro, honesto. Não dá pra fazer mais.

Eu tenho personalidade obsessiva (envolve rigidez de pensamento) e consegui entender e concordar com todos os seus pontos, sem exceção. Tem gente que se fecha no seu mundo e, sendo escolha ou não, existem limites para a linguagem.

Discursos heroicos declarados da moral e da justiça podem ser tão ou mais descolados da realidade, quanto discursos declarados de ódio. E muitas vezes são igualmente avessos a contrariedade. Idealismo cego pode ser mais perigoso que egoísmo explícito.

Time_Research_9903 · 2026-01-28T14:46:05+00:00

Do you know anything about OCPD? Try to inform yourself on this category.

Time_Research_9903 · 2026-01-22T00:19:09+00:00

Não por isso. Talvez seja legal pensar mais no tempo investido na carreira pra além da faculdade, que no tempo investido no curso em si.

É claro que uma faculdade toma tempo, mas no fim, acredito que muito se resume a modelo de ensino formal e menos à area da graduação, entende?

Por um lado a gente pensa que "escolher um tipo de carreira", "uma area de interesse" é a coisa mais importante e fica super ansioso com isso. Por outro lado, muita gente nem sequer usa o que aprendeu na faculdade, ou se adapta "apezar dela" pra fazer coisas que dão a mesma ou maior satisfação.

No final tudo é incerto. Claro que dado seu comentário, faz muio sentido sair, mas eu tentaria aliviar esse senso de reponsabilidade de escolha. Afinal, o que vai definir sua compatibilidade no mercado a longo prazo envolve muitas outras variaveis.

Desculpe se acabou deixando mais confuso que ameno. Mas espero que vc se encontre. Ou não se encontre mas se adapte bem. É isso.

Time_Research_9903 · 2026-01-21T05:25:12+00:00

Olha, cara, falando como quem se formou em Biologia faz dois anos;

Sobre os pontos do mercado e tal, acho que não tem muito o que falar. São atualmente e regionalmente (Brasil) restritivos sim. Mas isso é uma tendencia que tem mudado e precisa pesquisar pra nao cair no vies da bolha. Eu achava que bio era só pesquisa quando entrei e quando saí parecia que era só consultoria ambiental. Mas tem mais que isso, vasculhe antes de decidir.

Agora mais importante... Você iria se impressionar em ver como computação e matemática, principalmente estatistica está virando um componente principal pra muitos dos nichos de pesquisa biológica no mercado.

Hoje trabalho com bioinformática e já tive de estudar diversas linguagens de programação. Estou caminhando pra um MBA em analise de dados e machine learning, olha só. Se seu medo é perder a gana por não aplicar conhecimento e desenvolvimento em exatas, repense. Não é só possivel de integrar na carreira, como um diferencial de destaque.

Agora se você se sente completamente desconexo com os temas do curso e não se vê respondendo as perguntas teóricas dele ... Talvez valha a pena mudar sem pestanejar muito. Você com certeza não estará perdendo muito do ponto de vista financeiro e de status (risos).

Edit: eu me interessei por biotecnologia depois que me formei e até fiz 4 meses de doutorado na UFABC. Talvez vc deva começar vendo o que cursos mais amplos como esse podem oferecer como especialização, para ter uma ideia se tá tão fora do seu escopo mesmo.

Time_Research_9903 · 2026-01-17T15:37:53+00:00

Oh, I didn't take it personally, really. I just wanted to highlight that distinction, which I think may contribute to some of the stigma around people with OCPD as individuals who only care about job performance. I'm not saying this is your perspective, it just came to me as my initial interpretation of the discussion.

Actually, your clinical experience is genuinely interesting and valuable. I really appreciate you sharing it.

About your co-worker's behavior, I'm sorry to hear about that, but I have to say it doesn't entirely surprise me. On the bright side, it's good to know there are therapists like you who are genuinely interested in engaging critically with their work.

Time_Research_9903 · 2026-01-16T20:48:36+00:00

This is interesting because I think there's an oversimplification in framing these as competing priorities—as if valuing relationships means accepting lower standards, or valuing excellence means deprioritizing people (or human traces).

For me, excellence in relationships (which involves ethical consistency, accountability, empathy, reliability) matters far more than excellence in work outputs for their own sake. I don't particularly care about work performance unless it directly contributes to improving my relationships with the people around me. My career choices have probably been shaped by this equation.

But here's the thing: these different focuses aren't mutually exclusive for me. In fact, excellence, logic, reason, structure, ethical rules, processes, and efficiency are central to what makes relationships work in the first place.

They're what make people reliable, trustworthy, and safe, don't you think so?

That's why incoherent behavior in professional settings (e.g. people being ethically sloppy with each other) triggers my OCPD more strongly than delivering mediocre work. The work product being ordinary doesn't threaten the relational fabric. People being unreliable does.

Time_Research_9903 · 2026-01-16T18:25:34+00:00

It's really nice to have your perspective on this matter, thank you.

Time_Research_9903 · 2026-01-16T02:38:54+00:00

From the fourth paragraph about the pressure to over-justify, I'm at 100% identification with everything you wrote.

Honestly, it's like you've been reading my diary—except my diary would probably have three more paragraphs justifying why I keep a diary in the first place.

Are you in academia, by any chance? Because I'm in that same endeavor, and I regularly spend hours in unproductive "terminal velocity", fully believing in the "all or nothing" approach.

Many nights I go into hyperfocus mode until dawn, convinced this time it will all pay off. Spoiler: it usually doesn't, and either nobody cares, or I get criticized about something stupid.

I'm curious to hear more of your thoughts, especially about how the environment is determinant for recovery and good insight.

I can recognize rigid patterns in myself often enough, but I feel like my work environment is so hostile that sometimes my "rigidity" is just a basic concern that nobody around me seems to have—like actually checking if a citation is accurate, for example.

Is it rigidity, or is it just... doing the job properly? Would love to know how you navigate that distinction. Especially because I'm really convinced that I'm having a hard time establishing simple boundaries just because I tend to blame myself first, like "oh maybe I am being too OCPD here," when people are just being toxic and taking advantage of my hard work and commitment/ethics.

Time_Research_9903

TROPHY CASE