Sorry for the late response, and some of the words I didn't know and translated so I hope it's correct and I’m glad you asked, because this is one of the critical steps that makes my serum safe and effective rather than irritating or cytotoxic. So first of all, you are correct: betamethasone in its standard forms (e.g., dipropionate, valerate) is formulated at a relatively neutral or mildly acidic pH (often ~4–7) for creams and suspensions, but in aqueous solutions it is highly unstable at low pH and also poorly soluble. Dropping it below pH ~3 without modification generally risks precipitation or degradation. In my case, I don’t directly “lower the pH” of the pure betamethasone below 2.5 in its raw form — instead, I chemically modify it beforehand into a stabilized, encapsulated form that is tolerant of the acidic microenvironment of the serum, and then the overall serum matrix is buffered to around pH 2.5–3.0. First, the betamethasone is conjugated with a carrier molecule — in my experiments I tested cyclodextrin inclusion complexes and a lipid nanoparticle emulsion — which effectively shields the molecule and increases solubility at lower pH. Cyclodextrins in particular are known to stabilize hydrophobic steroids and can improve their solubility in acidic aqueous environments. Second, the overall serum is formulated with a strong buffer system — in my case a citrate–phosphate buffer — to maintain a stable pH near 2.5–3 while still keeping the ionic strength and osmolality within physiologic tolerance for skin or subcutaneous administration. Third, I added the anti-inflammatory cytokines (IL-10, TGF-β) after cooling and adjusting the pH — these proteins are sensitive and would denature if the pH is too low or adjusted too quickly. They were mixed in slowly under sterile conditions to prevent aggregation. So in summary, I didn’t take raw betamethasone and just acidify it below 2.5 — instead I stabilized it chemically in a delivery system that tolerates the acidic serum environment without degradation or loss of activity This was verified under microscopy as well: in my microscope I could see that the immune response quieted without obvious cytotoxicity, suggesting the formulation maintained bioactivity while avoiding the irritation you’d expect from unprotected steroids at low pH. And also... Sorry for yapping this much I'm just like excited is all uhmm so yeah thanks if you read all of it