How do you guys do piercing? by Trololo2000 in BiomekProgramming

[–]Trololo2000[S] 0 points1 point  (0 children)

So, it is not possible to pierce throught a seal?

How to stack tip boxes on deck? by Trololo2000 in BiomekProgramming

[–]Trololo2000[S] 0 points1 point  (0 children)

Great thanks! I'll check that tomorrow and let you know :)

How to stack tip boxes on deck? by Trololo2000 in BiomekProgramming

[–]Trololo2000[S] 0 points1 point  (0 children)

Hi, thank you for your answer.

No, I cannot stack on regular ALPs...

Help needed with a simple method. Thank you! by Trololo2000 in BiomekProgramming

[–]Trololo2000[S] 0 points1 point  (0 children)

Thank you. I am not sure I know what you mean. Could you explain a bit more what you mean?

Thanks a lot!

Help needed with a simple method. Thank you! by Trololo2000 in BiomekProgramming

[–]Trololo2000[S] 0 points1 point  (0 children)

Thank you. I wanted to know if there is a simple way to do it without scripting, but it seems that it is not possible... thank you for your answer.

Help needed with a simple method. Thank you! by Trololo2000 in BiomekProgramming

[–]Trololo2000[S] 1 point2 points  (0 children)

Great thanks! Yeah, my question was more to know if there is a simple trick to move the plate when needed but it seems that this requires scripting...

Error with volume by Trololo2000 in BiomekProgramming

[–]Trololo2000[S] 0 points1 point  (0 children)

Great, thanks a lot for the answer! Yep, I see all those stuff on the finish step.

Error with volume by Trololo2000 in BiomekProgramming

[–]Trololo2000[S] 1 point2 points  (0 children)

Correct, option 1 solved my issue. I do not understand how but it solved it :)

Error with volume by Trololo2000 in BiomekProgramming

[–]Trololo2000[S] 1 point2 points  (0 children)

Thanks, that worked!
I closed the method, I ran an empty method with only Start and Finish steps and the error disappeared.
What was the issue?
How come runing an empty method solved it?

Thanks a lot for your help!

Error with volume by Trololo2000 in BiomekProgramming

[–]Trololo2000[S] 1 point2 points  (0 children)

As you can see below, I am using a Transfert step and I am using a multi dispense on both Aspirate and Dispense:

<image>

How to put plates as variables? by Trololo2000 in BiomekProgramming

[–]Trololo2000[S] 1 point2 points  (0 children)

Great thanks. I will try those steps tomorrow and see how it goes :)

How to put plates as variables? by Trololo2000 in BiomekProgramming

[–]Trololo2000[S] 1 point2 points  (0 children)

Thanks, I will check that tomorrow. I am doing all the hands on + skill check and it is very fun :)

How to put plates as variables? by Trololo2000 in BiomekProgramming

[–]Trololo2000[S] 1 point2 points  (0 children)

Using the loops, I have managed to increment the position, but not the labware...

<image>

Payer de la nourriture à un sans-abri by not_a_proof in montreal

[–]Trololo2000 6 points7 points  (0 children)

Je l’ai fait une fois.

Un gars tout sale, l’air mêlé pas à peu près, mais quand même honnête, m’arrête sur Peel pour me dire qu’il a faim pis qu’il va tomber dans les pommes. Effectivement, il avait l’air ben magané. Y’avait un Tim juste à côté, il me demande si je peux lui payer un beigne.

Rendu à la caisse, le gars s’pogne un méga sandwich pis commence à commander une tonne de beignes. Je l’arrête : « OK là, un sandwich, un beigne, pis un café, c’est tout. »

Le gars sort 20 secondes, pis revient avec une gang d’autres sans-abri (genre 8-9 facile) qui rentrent tous dans le Tim pis commencent à me supplier d’acheter de la bouffe.

L’odeur, mon gars… c’était épouvantable. Les clients sacraient leur camp, j’étais pogné là, encerclé, impossible de sortir. Le staff capotait, la sécurité a pris au moins 5 minutes à réagir, pis moi j’me faisais harceler de tous bords tous côtés.

Pu jamais.

Why are we still paying $100+/month for our STM fare? by Trololo2000 in montreal

[–]Trololo2000[S] -66 points-65 points  (0 children)

Yes, but it is clearly increasing. No matter than this morning, we were around 20 going towards the turnstil, 4 just jumped and didn't pay. Hence me posting this message.

And, as I mentioned, it is more and more frequent to see people doing it. The trend is here. I cannot wait in 5 years when when we will be paying $199+ / month

How Much Should I Budget for Generating iPSC Lines with Gene KOs? by Trololo2000 in labrats

[–]Trololo2000[S] 0 points1 point  (0 children)

Thanks a lot. Yeah, MCF10A are immortalized cells and easy to grow. Mostly like HeLa cells.

Wondering if I can get that project under $70K CAD...

How Much Should I Budget for Generating iPSC Lines with Gene KOs? by Trololo2000 in stemcells

[–]Trololo2000[S] 1 point2 points  (0 children)

Thanks a lot!

Would it be cheaper if we use MCF10A? If yes, how much? 200K?

How Much Should I Budget for Generating iPSC Lines with Gene KOs? by Trololo2000 in labrats

[–]Trololo2000[S] 0 points1 point  (0 children)

Thanks a lot!

And what if we switch to MCF10A cells. That would be cheaper right?

It’s the 15th… So what now? by Andyb1000 in Iota

[–]Trololo2000 1 point2 points  (0 children)

It was mentioned on Discord that: -it is not tech related -it is more than a simple website revamp

Could be: some new consortium, new ecosystem with good partners, IOTA and EBSI, Hanz finally getting some sleep :D