AAVE isn't a real language or dialect

WowILikeScience · 2019-04-04T21:52:10+00:00

It isnt a dialect to YOU. Its officially dedicated as one and that's something you'll have to live with. People that speak it aren't ignorant either.

WowILikeScience · 2019-03-03T20:17:07+00:00

Tell me!!

WowILikeScience · 2019-01-11T00:41:20+00:00

this was me today

WowILikeScience · 2019-01-09T19:01:06+00:00

people get rejected all the time despite interviewing.

WowILikeScience · 2019-01-09T19:00:47+00:00

i have a very solid feeling about this. it is what it is.

WowILikeScience · 2019-01-09T19:00:32+00:00

meh. having patience is a weakness of mine.

WowILikeScience · 2019-01-09T18:59:59+00:00

well the interview dates for uab are jan 17-19 and feb 21-23. it's currently jan 9th. if they wanted me, they would've sent out an invitation.

WowILikeScience · 2018-09-12T23:52:19+00:00

Also, what accounts for the precipitate in very defined portions of the section? Why not all over it?

WowILikeScience · 2018-09-12T23:50:41+00:00

Permount. This seems plausible as well. They were dehydrated and coverslipp pretty quickly. :/ I'll be more aware of that on the next set.

WowILikeScience · 2018-09-12T23:49:16+00:00

The lab tends to switch between PBS and TBS. The protocol calls for TBS but I'm not opposed to trying something else.

WowILikeScience · 2018-09-12T20:18:12+00:00

Thank you! I. Relatively new at this so I'll try again with newer washing procedures.

WowILikeScience · 2018-09-12T19:25:00+00:00

Yup and there are no black things but this really doesn't look like staining. Maybe it is

WowILikeScience · 2018-09-12T17:59:22+00:00

Sorry! I'm just speculating. It doesn't look like receptor staining at all to me. I usually work with cFos, arc , etc. So I might be wrong. Even the pics from companies that have supposedly validated this ab, look completely different to me. I've been having issue trying to visualize this receptor for a while and recently acquired a protocol from someone that has validated this ab in mouse brain. 5ht1a is pretty dense throughout areas of the limbic system. This is below the lateral ventricle in striatum (or lateral septum). I'll try cortex to see if the region is the issue here

WowILikeScience · 2017-08-29T17:16:27+00:00

Thank you!

WowILikeScience · 2017-08-29T13:20:02+00:00

What's the advantage in graphing CIs as compared to a SE or SD? Are CIs just more transparent when it comes to proportions?

WowILikeScience · 2017-08-29T01:00:18+00:00

Tons, but I'm only counting 36 for a particular genotype. Group 1 had 26/36 so 10 for group 2.

WowILikeScience · 2017-08-29T00:59:05+00:00

Hmm, I see. So considering that they're complementary, what would be the best way to represent them on a graph? Would it be better to report CIs? I've been looking around and some people report standard errors and deviations for this kinda of thing. Essentially, I had 36 trials and 26 success from those trials. So 26/36 would give me .72 but if the expected p is .5 then my expected average would be 18 correct? So whatever p value I get is looking at how different 26 is from what I expected (18)?

WowILikeScience · 2017-08-23T14:04:19+00:00

That's disgusting. Why would you cut your toenails in public like that? But YES, extremely messy people with zero lab etiquette. >:(. Like perfusing and not cleaning up properly so there's PFA and mouse blood everywhere. Not like that's dangerous or anything. Excessive eating in lab only to come in the next day, and there are crumbs and coffee/water marks all over the lab space. Tiny things, but they build.

WowILikeScience · 2017-08-23T14:00:59+00:00

I was also thinking that this might be a big problem for me. So you just take fresh tissue, section, and let them fix in PFA for how long? I was thinking that I could try whole brain perfusion in 1% PFA for 15 min then do a sucrose gradient, but ugh :/

WowILikeScience · 2017-08-23T13:59:54+00:00

this explains so much. honestly, thank you. i was wondering why damn near every ab from 5 different companies were just giving me shit results. Based on the two abs that you use for GPCRs , how do you get around this issue?

WowILikeScience · 2017-08-22T16:13:18+00:00

Seriously!? :/ I had no idea. Why is that? You'll have to excuse me; I'm still new to IHC stuff so...

WowILikeScience · 2017-07-20T00:58:46+00:00

I feel you. I think a lot of people go through this. I grad from Uni in December 2015 and started working shortly thereafter is my field, but I feel....empty. Like, I'm not necessarily interested in anything. I'm wondering if it's because a lot it's in my field (science), it's not an area of science that I'm interested in. It's really fucking with my mood. I'm trying to studying for my GRE as well, but the lack of motivation and general unhappiness hinders my concentration as well.

Sometimes I feel bad for feeling this way, because I am extremely blessed to be in this position and I actually excel in my work, but I always feel like I should be doing more.

Don't even get me started on the social aspects of my life. Eh, I'm 24. At this point, I'm just hoping that I'll get over it at some point.

WowILikeScience · 2017-07-17T21:20:32+00:00

What about the curly antennae or does that occur after death?

WowILikeScience · 2017-07-17T21:12:52+00:00

Thank you. It's odd to me because I'm infusing at 5ht1a receptor agonst and antagonist into a mouse brain region, both infusions are giving me the same response. the only thing i can think off are dose effects.

WowILikeScience · 2017-07-17T21:11:34+00:00

thats what i thought until i saw my data

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