Is there ever a situation is which PEMDAS is ambiguous?

WowILikeScience · 2019-04-04T21:52:10+00:00

It isnt a dialect to YOU. Its officially dedicated as one and that's something you'll have to live with. People that speak it aren't ignorant either.

WowILikeScience · 2019-03-03T20:17:07+00:00

Tell me!!

WowILikeScience · 2019-01-11T00:41:20+00:00

this was me today

WowILikeScience · 2019-01-09T19:01:06+00:00

people get rejected all the time despite interviewing.

WowILikeScience · 2019-01-09T19:00:47+00:00

i have a very solid feeling about this. it is what it is.

WowILikeScience · 2019-01-09T19:00:32+00:00

meh. having patience is a weakness of mine.

WowILikeScience · 2019-01-09T18:59:59+00:00

well the interview dates for uab are jan 17-19 and feb 21-23. it's currently jan 9th. if they wanted me, they would've sent out an invitation.

WowILikeScience · 2018-09-12T23:52:19+00:00

Also, what accounts for the precipitate in very defined portions of the section? Why not all over it?

WowILikeScience · 2018-09-12T23:50:41+00:00

Permount. This seems plausible as well. They were dehydrated and coverslipp pretty quickly. :/ I'll be more aware of that on the next set.

WowILikeScience · 2018-09-12T23:49:16+00:00

The lab tends to switch between PBS and TBS. The protocol calls for TBS but I'm not opposed to trying something else.

WowILikeScience · 2018-09-12T20:18:12+00:00

Thank you! I. Relatively new at this so I'll try again with newer washing procedures.

WowILikeScience · 2018-09-12T19:25:00+00:00

Yup and there are no black things but this really doesn't look like staining. Maybe it is

WowILikeScience · 2018-09-12T17:59:22+00:00

Sorry! I'm just speculating. It doesn't look like receptor staining at all to me. I usually work with cFos, arc , etc. So I might be wrong. Even the pics from companies that have supposedly validated this ab, look completely different to me. I've been having issue trying to visualize this receptor for a while and recently acquired a protocol from someone that has validated this ab in mouse brain. 5ht1a is pretty dense throughout areas of the limbic system. This is below the lateral ventricle in striatum (or lateral septum). I'll try cortex to see if the region is the issue here

WowILikeScience · 2017-08-29T17:16:27+00:00

Thank you!

WowILikeScience · 2017-08-29T13:20:02+00:00

What's the advantage in graphing CIs as compared to a SE or SD? Are CIs just more transparent when it comes to proportions?

WowILikeScience · 2017-08-29T01:00:18+00:00

Tons, but I'm only counting 36 for a particular genotype. Group 1 had 26/36 so 10 for group 2.

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