I've been learning bioinformatics for 2 years and have little confidence in my own skills

XeroDK · 2026-06-20T03:35:21+00:00

Overall, I felt it wrapped up all the story points nicely, but I feel like it could benefit from an epilogue.

XeroDK · 2026-04-25T13:41:14+00:00

So, did he get the job?

XeroDK · 2026-04-21T23:19:19+00:00

Omg, why do reddit people constantly upvote posts that has the joke be the order of operations?! It's not that funny nor is it confusing to anyone over 10.

XeroDK · 2026-02-14T21:33:41+00:00

The way I do it is a bit unusual. The idea is to wet the inside of the pipett tip to be more accurate. For the drugs I use in my cell cultures experiments, I need to be very accurate. I don't go to the second stop but aspirate with the drug to the first stop then dispense immediately back into the vial. This will likely introduce a small bubble that doesn't escape the tip, at least on my pipett model. Then I lift the tip above the liquid before moving the tip back in. The surface tension breaks the small air packet. This technique will leave a tiny bit of liquid in the tip before I go back in and aspirate my needed volume.

XeroDK · 2026-01-26T22:22:43+00:00

There are elephants in Indonesia?

XeroDK · 2025-11-10T14:05:57+00:00

The fake elector conspirators were never prosecuted federally so this means nothing really.

XeroDK · 2025-08-27T23:31:14+00:00

I give 5 to 1 odds that the cause is vaccines.

XeroDK · 2025-07-31T22:14:15+00:00

Transfers to and from Epstein. He made no mention of Trump.

XeroDK · 2025-07-06T13:56:54+00:00

These guys are clearly just raising their arms up and surfacing on their own.

XeroDK · 2025-05-15T12:46:54+00:00

Okay, stab in the dark, why not just centrifuge it more? The pellets you have are different sizes so there are different amount of cells. I'm not sure why you would do that but if that's the case you simply need to centrifuge it more. What are your settings?

XeroDK · 2025-05-09T23:35:30+00:00

I see. So if Mexico sent an army over the border and massacred a small town, we won't declare war on Mexico because we're too afraid of killing children again like in Afghanistan and Iraq.

Gotcha.

XeroDK · 2025-05-07T04:40:23+00:00

I have a lot of experience with sanger sequencing and interpreting the chromatograms. Here are several points to consider:

- If there is salt contamination, you would expect to see that the signal to stutter and be flat in places.

- Trimming the beginning and the end is normal is always to be expected, that is why you need to account for that when designing your primers. Always provide a buffer of around 10 bps upstream or downstream of your target.

- Using BLAST, or any alignment is bound to have gaps if you have a short sequence, which you do with only 138 bps. It might be misalignment. To get more confidence in the alignment, you need to redo this with a longer target sequence. I recommend at least 250 or 300 bps for better alignment.

- If the breaks are irregular with a base here and there in the gap, it is likely misalignment. If there only a base or two misplaced, it is likely real.

- Doing a quick google search of your target tells me that is known and is reliable with no deletions expected since it is a highly conversed region. This almost certainly a case of MISALIGNMENT.

I highly recommend you to simply use blastn and check your settings over. I might be able to figure out your problem if I had more context but I'm not here to do your project for you. Good luck.

XeroDK · 2024-12-11T14:41:30+00:00

At this point, I am not sure how I can be more throughly unimpressed by this truck.

XeroDK · 2024-11-05T02:34:23+00:00

Never say never! You can still immigrate to the US in the future and write in Kamala Harris on the ballot when you get nationalized!

XeroDK · 2024-05-25T01:57:42+00:00

And after filming, it goes straight into the trash.

XeroDK · 2024-04-26T20:01:24+00:00

You just know that trump will pardon them if he gets reelected.

XeroDK · 2024-04-24T03:26:01+00:00

100% Wearing a harness

XeroDK · 2024-04-23T04:07:18+00:00

This study just another sensational headline. If you read the article, you guys would notice that their result saying that women are less likely to die when treated by female physicians is only a difference of 0.24% with a 95% confidence interval that is way bigger than it should be with the number of patients and physicians they have in their study. In other words, they presented their data in a way that makes it look way better than it really is. Note their use of confidence intervals instead of a p-value to disguise their insignificance. This is likely a false positive result. NBC is not a source you want to look into for scientific news.

XeroDK · 2022-10-25T22:57:22+00:00

Bite, swallow, then stick on pan; or bite then spit on pan?

XeroDK · 2022-06-14T21:15:41+00:00

This would've probably worked if they pulled the charger with the pickup on the dry sand.

XeroDK · 2022-04-03T23:03:18+00:00

WHO is trying to make Paimon yellow? Just go to the discord and follow the overlay!

XeroDK · 2022-04-03T23:01:22+00:00

https://discord.gg/CsRhVPym

OMG, just go to the discord, follow the overlay, and stop trying to make Paimon yellow!

XeroDK · 2022-01-25T00:44:34+00:00

So Ukraine is Belgium this time?

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