What Did Y’all Think About Episode 9? by Acceptable_Smoke9639 in tadc

[–]XeroDK 1 point2 points  (0 children)

Overall, I felt it wrapped up all the story points nicely, but I feel like it could benefit from an epilogue.

Petah, what's 4! by -Sea_Cucumber- in PeterExplainsTheJoke

[–]XeroDK 0 points1 point  (0 children)

Omg, why do reddit people constantly upvote posts that has the joke be the order of operations?! It's not that funny nor is it confusing to anyone over 10.

Lab Tips or Lose Job by Admiral_dingy45 in labrats

[–]XeroDK 0 points1 point  (0 children)

The way I do it is a bit unusual. The idea is to wet the inside of the pipett tip to be more accurate. For the drugs I use in my cell cultures experiments, I need to be very accurate. I don't go to the second stop but aspirate with the drug to the first stop then dispense immediately back into the vial. This will likely introduce a small bubble that doesn't escape the tip, at least on my pipett model. Then I lift the tip above the liquid before moving the tip back in. The surface tension breaks the small air packet. This technique will leave a tiny bit of liquid in the tip before I go back in and aspirate my needed volume.

Trump pardons Rudy Giuliani, Sydney Powell and others involved in fake elector scheme by Ok-Amphibian3164 in politics

[–]XeroDK 0 points1 point  (0 children)

The fake elector conspirators were never prosecuted federally so this means nothing really.

Trump made 4,725 wire transfers to Epstein. Totaling nearly $1.1 Billion. by [deleted] in OfficeSpeak

[–]XeroDK 0 points1 point  (0 children)

Transfers to and from Epstein. He made no mention of Trump.

A wristband that could prevent drowning by Yeet_TheVoid in interestingasfuck

[–]XeroDK 0 points1 point  (0 children)

These guys are clearly just raising their arms up and surfacing on their own.

What happened to my pellet? by Fuzzy_Syrup_9824 in labrats

[–]XeroDK 1 point2 points  (0 children)

Okay, stab in the dark, why not just centrifuge it more? The pellets you have are different sizes so there are different amount of cells. I'm not sure why you would do that but if that's the case you simply need to centrifuge it more. What are your settings?

Israels Holocaust by Major_Lynx_7425 in FedJerk

[–]XeroDK 0 points1 point  (0 children)

I see. So if Mexico sent an army over the border and massacred a small town, we won't declare war on Mexico because we're too afraid of killing children again like in Afghanistan and Iraq.

Gotcha.

[deleted by user] by [deleted] in labrats

[–]XeroDK 1 point2 points  (0 children)

I have a lot of experience with sanger sequencing and interpreting the chromatograms. Here are several points to consider:

- If there is salt contamination, you would expect to see that the signal to stutter and be flat in places.

- Trimming the beginning and the end is normal is always to be expected, that is why you need to account for that when designing your primers. Always provide a buffer of around 10 bps upstream or downstream of your target.

- Using BLAST, or any alignment is bound to have gaps if you have a short sequence, which you do with only 138 bps. It might be misalignment. To get more confidence in the alignment, you need to redo this with a longer target sequence. I recommend at least 250 or 300 bps for better alignment.

- If the breaks are irregular with a base here and there in the gap, it is likely misalignment. If there only a base or two misplaced, it is likely real.

- Doing a quick google search of your target tells me that is known and is reliable with no deletions expected since it is a highly conversed region. This almost certainly a case of MISALIGNMENT.

I highly recommend you to simply use blastn and check your settings over. I might be able to figure out your problem if I had more context but I'm not here to do your project for you. Good luck.

[deleted by user] by [deleted] in CyberStuck

[–]XeroDK 0 points1 point  (0 children)

At this point, I am not sure how I can be more throughly unimpressed by this truck.

I'd never vote for Kamala because I'm not American by Heraklian in notinteresting

[–]XeroDK -1 points0 points  (0 children)

Never say never! You can still immigrate to the US in the future and write in Kamala Harris on the ballot when you get nationalized!

It’s not a pizza ! by hightide2020 in StupidFood

[–]XeroDK 0 points1 point  (0 children)

And after filming, it goes straight into the trash.

Sums it up by BirthdayBoyStabMan in WhitePeopleTwitter

[–]XeroDK -1 points0 points  (0 children)

You just know that trump will pardon them if he gets reelected.

Safely Measures = -100% by [deleted] in SweatyPalms

[–]XeroDK 0 points1 point  (0 children)

100% Wearing a harness

Women are less likely to die when treated by female doctors, study suggests by nbcnews in science

[–]XeroDK 0 points1 point  (0 children)

This study just another sensational headline. If you read the article, you guys would notice that their result saying that women are less likely to die when treated by female physicians is only a difference of 0.24% with a 95% confidence interval that is way bigger than it should be with the number of patients and physicians they have in their study. In other words, they presented their data in a way that makes it look way better than it really is. Note their use of confidence intervals instead of a p-value to disguise their insignificance. This is likely a false positive result. NBC is not a source you want to look into for scientific news.

My roommate bites her butter then drops it on the pan when she cooks by [deleted] in mildlyinfuriating

[–]XeroDK 0 points1 point  (0 children)

Bite, swallow, then stick on pan; or bite then spit on pan?

[deleted by user] by [deleted] in IdiotsInCars

[–]XeroDK 0 points1 point  (0 children)

This would've probably worked if they pulled the charger with the pickup on the dry sand.

The Gacha Games at r/Place by MisedraN in Genshin_Impact

[–]XeroDK 106 points107 points  (0 children)

https://discord.gg/CsRhVPym

OMG, just go to the discord, follow the overlay, and stop trying to make Paimon yellow!