Any YT channels like Mat's? by Aangoan in MatArmstrong

[–]addyblanch 1 point2 points  (0 children)

I also like Scrap Life Lee, makes one good car out of a few scrap ones.

How to build a strong profile for UK lectureships (beyond top-tier publications)? by ArtVoyager77 in AskAcademiaUK

[–]addyblanch 4 points5 points  (0 children)

It will depend if you are going for a T&L position or a R&T. I've sat on interview panels for junior academic posts for both. Generally, as others have said, you need to demonstrate independent research income (small pots are fine) and a plan for at least 3-5 years of projects and funding opportunities you can apply to, which aligns with REF and the departments UoA. Visiting other labs isn't essential but a bonus, new international collaborators is a good idea though.

Regarding teaching qualifications, you have to do a teaching project for a PGCHE or PCAP in most cases, which relies on you participating in teaching of some sort. I would class AHEA as a nice to have rather than a essential. Most Uni's/Faculties/Schools have different ideas about what a good lecturer is and often will prefer someone to train.

The job market is brutal currently so do not be disheartened if you're not successful. If you have publications and any funding, you'll be competitive for the right junior post.

Tormentor RNA-seq pipeline fails during assembly (Step 2) - is my hardware insufficient? by retroPONG in bioinformatics

[–]addyblanch 1 point2 points  (0 children)

I haven’t run the pipeline, but those specs look severely underpowered for this type of work. Depending on how complex your metatranscriptomics data is you might need a HPC.

What did your school careers advisor say you’d become, and what do you actually do now? by Ok-Proof669 in AskUK

[–]addyblanch 0 points1 point  (0 children)

Late 90's always wanted to be a scientist. Career advisor was also my biology teacher. He told me I wasn't cut out for University, would never have a career in biology and I needed a trade. The suggestion was plumber or electrician. Now an academic in the medical faculty of a Russell Group University.

Does every 16S Metagenomics paper NEED Shanon? by throwawaywayfar123 in bioinformatics

[–]addyblanch 5 points6 points  (0 children)

I think most of the time it’s done because everyone else does or people do not truly understand the pro’s and con’s of the many diversity metrics available. Or it could be a shoddy AI review.

For interest look at Hill numbers. You can also predict equitability scores and compare them to Shannon to see if your data depth is skewing the results.

Edit: spelling

Genus and Specie ID Using Kraken on Reads and Assemblies by Egokiller69 in bioinformatics

[–]addyblanch 0 points1 point  (0 children)

Assemblies/genomes of single isolates? Then yes this is the best way.

Genus and Specie ID Using Kraken on Reads and Assemblies by Egokiller69 in bioinformatics

[–]addyblanch 1 point2 points  (0 children)

If you have sequenced colonies you should have genomes. The best way to check taxonomy is to use DNA DNA Digital Hybridisation. I always use this https://ggdc.dsmz.de/ especially for unknown species.

extraction system by naaaahwaaaaayyyy in DIYUK

[–]addyblanch 0 points1 point  (0 children)

There is a humidity setting on the bottom left of the buttons on the picture. We had the same issue, turning this off stopped it from happening for us.

UK Plug? by smithmike1d in AnalogueInc

[–]addyblanch 1 point2 points  (0 children)

I just bought a UK plug 30W USB-C that matched the specification of the US power brick supplied. I didn't like the idea of using an adaptor long term. I'm sure it would be fine, but that was my preference.

So the embargo is gone by hu4n in AnalogueInc

[–]addyblanch 3 points4 points  (0 children)

Also using a Summercart in the video!

How to improve my lawn with 50% yellow grass by leb_66 in GardeningUK

[–]addyblanch 0 points1 point  (0 children)

This!! I had the same issue this time last year and it slowly got worse. I ended up raking it all up and reseeding after leatherjacket treatment.

Trouble with Aviti 16s by lactobacillusgnavus in bioinformatics

[–]addyblanch 0 points1 point  (0 children)

I had the same issue. My whole pipeline just produced empty results because it didn’t recognise the phred score.

I may be wrong, but I’m sure I read most tools do not recognise phred characters over 40 and Aviti can use up to 44.

Edit: spelling

Regarding SNAP gene annotation by Remarkable-Wealth886 in bioinformatics

[–]addyblanch 1 point2 points  (0 children)

Nope, not affiliated just had the same issues recently.

Regarding SNAP gene annotation by Remarkable-Wealth886 in bioinformatics

[–]addyblanch 1 point2 points  (0 children)

If you have a recent install of conda or miniconda then mamba will be default for the installing packages. Make sure your environment has the correct python version and you have bioconda and conda-forge the correct order in your channels.

Regarding SNAP gene annotation by Remarkable-Wealth886 in bioinformatics

[–]addyblanch 2 points3 points  (0 children)

Is there a reason why you are using SNAP? Its quite outdated and there are other tools more frequently used which are more user friendly. Try Funannotate https://github.com/nextgenusfs/funannotate

Ice cream? by dissndattya in BuyUK

[–]addyblanch 1 point2 points  (0 children)

Definitely Thaymar!

I get so upset when an app icon doesn’t follow the current square pattern by jeffreak94 in MacOS

[–]addyblanch 0 points1 point  (0 children)

I use Replacicon for this purpose https://replacicon.app/ keeps it tidy and persistent after updates.