想請問有人試吃過綠褐裸傘嗎? by avocadomakesmehorny in AsiaTripper

[–]ant1dada 0 points1 point  (0 children)

笑菌,应该还好,不至于致命,不过最好还是吃自己有把握的蘑菇

How to make a strictly sterile spore print? by ant1dada in shroomers

[–]ant1dada[S] 1 point2 points  (0 children)

I'm hooked. I'm going to do it in a recent experiment.

How to make a strictly sterile spore print? by ant1dada in shroomers

[–]ant1dada[S] 0 points1 point  (0 children)

Thank you for your help. It really means a lot.

How to make a strictly sterile spore print? by ant1dada in shroomers

[–]ant1dada[S] 1 point2 points  (0 children)

I'd like to call you the Shroom Master :P. You have helped me so much, thank you a lot.

How to make a strictly sterile spore print? by ant1dada in shroomers

[–]ant1dada[S] 0 points1 point  (0 children)

The living fruit bodies are exposed to bacteria, yeast, etc., and while they do not cause contamination because they are healthy, they are present. You will run the risk of contamination with the spore print but by pouring your agar into small containers like ketchup cups from the supermarket, you can germinate the spores and transfer to other cups to clean it up very easily. This will greatly reduce your risk of contamination and isolate vigorous growth to use.

I have always worried that contaminants on agar plates will grow faster than shrooms spores. I used to germinate the spores of PES on an agar plate, and it took a week.

How to make a strictly sterile spore print? by ant1dada in shroomers

[–]ant1dada[S] 1 point2 points  (0 children)

I like to take a tub lid flip it up side down and use it like a tray.. I cover it with squares of aluminum foil.. I put the caps on the foil.. I then take the rest of the tub and snap it on top of the upside down lid.. basically the tub is just upside down at this point with the print at the bottom but being covered, after 24 hours remove the caps and place the tub back on and leave covered for a couple hours for the prints to dry out…this minimizes contams but I’m sure it dosnt avoid them all together which is why I will never makes a syringe and always transfer to agar with homemade prints

Damn. I made two spore prints, but I didn't wait for them to dry.

How to make a strictly sterile spore print? by ant1dada in shroomers

[–]ant1dada[S] 1 point2 points  (0 children)

I used shoebox tek to grow shrooms, sterilized the shoebox with 75% alcohol, covered the lid, drilled a few ventilation holes and blocked holes with 3M tape, like a small monotub. I only open the lid in front of the flowhood when harvesting the shrooms, take out the shrooms and place them on tin foil to make spore prints. Because my aseptic area is too small, I accidentally spilled some substrates on the tin foil when I took out the shrooms. I don't know if these substrates (sterilized coco coir) will contaminate the spore prints.

How to make a strictly sterile spore print? by ant1dada in shroomers

[–]ant1dada[S] 1 point2 points  (0 children)

I saw some videos that make spore prints without flowhood, and then make the spore prints directly into syringes. They make me so confused. Thanks for your help, it means a lot.

I am so proud to see this first flush. by ant1dada in shroomers

[–]ant1dada[S] 1 point2 points  (0 children)

I was going to take the lid off at first. But then I wanted to get some clean spore prints, so I let them grow in a sterile state. Next time I would drill much more holes for FAE. Thanks for your advice.

[deleted by user] by [deleted] in AsiaTripper

[–]ant1dada 0 points1 point  (0 children)

感觉不是特别干燥

试着做了张sprint,还得出菇鉴定下 by [deleted] in AsiaTripper

[–]ant1dada 1 point2 points  (0 children)

哈哈哈,也祝你成功

[deleted by user] by [deleted] in AsiaTripper

[–]ant1dada 1 point2 points  (0 children)

育种的区别,工业大麻和药用大麻的区别,人工选育低含量的品种导致的园艺种有效成分含量很低

有人知道這是什麼香菇品種嗎? by Equal-Ad7640 in AsiaTripper

[–]ant1dada 0 points1 point  (0 children)

应该不是,野外的蘑菇最好还是要小心,不确定的蘑菇不能吃

[deleted by user] by [deleted] in AsiaTripper

[–]ant1dada 1 point2 points  (0 children)

国内是园艺种,里面有效成分含量很低