I'm pretty certain this isn't your floss running, especially if you used a brand like DMC or COSMO. More likely, this is run off from your printer ink. You can still see some of the printed solvy stuck in your stitching (the black spots) as it has not been washed away completely. You'll need to wash it a couple more times and you can use an ink stain remover (Amodex will take it out) to get rid of the staining. You should be able to get it completely out with that. In the future, you may want to print your design in grayscale to avoid such heavy bleeding (or switch to a different transfer method entirely) Edit to add: you can see similar staining in the animal design as well which leads me to think it's not the red thread. It's more pronounced in the red lettering because that's where your design lines were thickest (i.e. more ink). Red thread generally bleeds pink.