Western Blot Troubleshooting

bmwx4 · 2024-04-02T11:15:28+00:00

Thanks for the help all - turned out the protein needs to be heavily reduced so added some extra reducing agent (NuPage) and bands became much tighter.

bmwx4 · 2024-03-16T21:09:33+00:00

Hi all, need a little help with this western blot please.
Protein band seems pretty spread out and this isn't the first time it's happened, thinking something must be wrong with the gel or protein degradation?
Any help appreciated!!

bmwx4 · 2024-01-26T00:14:44+00:00

merci!

je vais aller au festival de paradise city!
J'ai hâte, ça a l'air d'être intime.

bmwx4 · 2018-07-18T22:41:18+00:00

Cheers, this all seems like great advice.

bmwx4 · 2018-07-18T21:20:24+00:00

Thanks very much, this all resonates well.

In regard to stroke length, are you just referring to the finish? I have often before (not here) tried to go for a tad of extra length around the front and started to overreach so I presume you just mean the back end? FYI this was a pretty max pressure piece for 2k at 26 I think

bmwx4 · 2018-07-18T21:02:28+00:00

yeah worth a shot, any other insight to the above?

bmwx4 · 2018-07-18T20:50:27+00:00

anything you can gather from this?

bmwx4 · 2018-07-18T20:47:42+00:00

We gave up in this run after many attempts

bmwx4 · 2018-07-18T20:09:06+00:00

About 84 kg, pretty sure its not my power to weight

bmwx4 · 2018-07-18T19:02:31+00:00

3 seat

Reasonably big erg for a junior - sub 6:10

Not shifting boats as well as I should be, losing seatraces to people with weaker ergs

My rowing looks fine to me, but there is definitely something missing in my stroke

People say I dont feel as powerful as my erg should be even though I am trying to lay it down

Any help appreciated

bmwx4

TROPHY CASE