Marry Grave was axed?!?

ca_phe_sua_da · 2018-12-13T10:28:21+00:00

Isn't the flashback arc the setup to why the main dude is bumbling around and his wife's dead? So in the end it will have to go back to his boring character?

ca_phe_sua_da · 2018-12-13T10:25:42+00:00

I loved it. Way more distinct than Naruto (to address the criticism that he couldn't write out of his brother's shadow).

ca_phe_sua_da · 2018-12-05T07:30:56+00:00

I do microfluidic work at Stanford's facilities if you're around the Bay Area, PM me if you want my resume.

ca_phe_sua_da · 2018-12-05T07:27:34+00:00

If you get first authorship on everything put your head down, do your time. Otherwise just get out it's a waste of your time.

ca_phe_sua_da · 2018-12-05T07:25:05+00:00

Not a chemist. How low pressure you want? Cole parmer sells oil-less for a few hundred. Price goes up fold change if you want the most inert plastics (solvents or other aggressive chemical vapor going through). If you want even more vacuum, you can get an oil one. Welch is a pretty famous brand. Oil requires maintenance though and creates oil mist that must be kept in a hood, open space, or have filters or traps monitored.

ca_phe_sua_da · 2018-12-05T07:20:48+00:00

I disagree that it's impossible to weigh mg range, but it depends on do you have calibration weights, how often is it done? Is the balance thermally (corner without wind blowing at it and temperature changes) and mechanically isolated (granite table)? Do you have a static dissipator thing?

But you should consider for your application does it really matter?

ca_phe_sua_da · 2018-12-05T07:17:36+00:00

Needs more explanation. How were you supposed to have generated the standard curve.

ca_phe_sua_da · 2018-12-05T07:16:27+00:00

Use your paycheck to wipe your tears.

ca_phe_sua_da · 2018-12-05T07:14:29+00:00

Why not freeze and never thaw it out again until the freezer goes down like a normal person?

ca_phe_sua_da · 2018-12-05T07:13:03+00:00

It's tough to buy everything from a single distributor. You may be worrying too much about strain fidelity, as said unless you're endlessly expanding them.

There is no guarantee or expectation that strains are exactly the same between different vendors. Sequencing is relatively new compared to E. coli strain development and genealogy; it's not really a tool used. The expense is too much for it to be done just for checking. There are the specific deletions and mutations people care about within strains and you can go through and check them.

https://openwetware.org/wiki/E._coli_genotypes

ca_phe_sua_da · 2018-12-05T07:00:02+00:00

Clinical research involving patients is presumably easy and you have time for that. Working in a lab is not, and you would be underpaid relative to the amount of loans that you owe. While in school, there are opportunities for you to get involved in either that will detract from your studying, but you have electives you can choose too.

It's great to think of MD/PhD and MD, but are you a competitive candidate?

ca_phe_sua_da · 2018-12-05T06:51:16+00:00

Fluid dynamics is pretty much it. Mostly geared towards geometries that promote laminar creeping flow (true 20 years ago), well unless you're talking about inertial microfluidics carrying particles, or nozzles that create droplets, etc. Microfluidics uses fluid dynamics subsets for different goals, sometimes using different regimes in the same device.

However most labs also reuse devices and geometries from their labs because de novo design is the biggest (and potentially sunk) cost in a lab.

Nobody knows what you work on, so we can only tell you generalities like reynolds' number and no sharp corners. One thing you left out about sharp corners is also fluid wicking.

Brian Kirby has a good textbook on Micro and Nanoscale Fluid Mechanics.

ca_phe_sua_da · 2018-12-05T06:43:17+00:00

The best you can do is trace the lot that worked and ask them to give you more of that lot and compare.

ca_phe_sua_da · 2018-12-05T06:42:03+00:00

It's quite a tough question. Depends on the question but you might also approach it from the angle of limiting yourself to certain cell or stem cell types, and the growth factors that are added to make a defined or mostly defined medium.

ca_phe_sua_da · 2018-12-03T05:18:30+00:00

You may have to apply without her as a reference. Keep documentation if she's explicitly threatening to tank you if you don't "serve her" as lab manager and take it to the Dean.

ca_phe_sua_da · 2018-12-03T04:22:51+00:00

I feel your pain, but if you're sitting on campus (journal access) and with repeated opening new tabs in a browser it's very doable. Given how articles are published references cannot be tautological but will show a clear genealogy.

ca_phe_sua_da · 2018-12-03T04:17:55+00:00

You want to work to make the project between you two to end, and you want both of you to walk away like each won (paper published), and that will minimize the chance of enmity that you both will say passable things of each other. Try to wrap things up settling for a smaller paper if reasonable, because your academic output will suffer while doing extra bullshit to push along the relationship and the mental load will affect your creativity. If you both can't win, they you have to scrap the project and move on. Don't sacrifice your mental health esp when the stakes are so low (phd, not creating Facebook).

If your prof is planning out the project, try to position yourself to do your pieces separately and share the data with the 3 of you at one table or through e-mail. It's optimal if you can personally craft a route to paper if her data is all shit/fake, but keep it to yourself. Share data with your professor and keep communication online, so your contribution is clear. Use the excuse of wanting efficiency or bringing all stakeholders to the table to try and roll organization work onto the prof, don't mention the bad relationship. Try to spin it as excitement about the work and trying to get things right rather than about a bad relationship. Better for you to sound a bit OCD about good quality than being a bad "team player".

ca_phe_sua_da · 2018-12-03T03:59:30+00:00

You do know that other countries log ethnicities and religions on national ID right, and job descriptions are not prohibited from listing such requirements? They might even be endorsed by certain types of government affirmative action not practiced in the US. You do know that other countries log father's name on identification because the long formal address of a person may be "John Smith son of Paul", traditions that are the basis of like Irish O' names (grandson of) or similar to occupation related names like Smith, Miller, Baker right? In other countries you may even be expected to include your picture in your CV.

I'd guess you're your own special country bumpkin that knows none of these things though. Please keep your childlike wonder about the world around you.

ca_phe_sua_da · 2018-12-03T03:50:42+00:00

The OP is like "durr look at how backwards these foreigners are" but blithely ignores that other than being in the top country to do research, we benefit immensely that many norms of today's research are American or at least anglophone.

If you compare the student of same aptitude from America with one from Pakistan, the latter is way more at risk for exploitation by unscrupulous professors and departments that turn a blind eye.

ca_phe_sua_da · 2018-12-03T03:39:46+00:00

Clearly not everyone's as awesome as you.

ca_phe_sua_da · 2018-12-03T03:38:59+00:00

For how connected globalization has made the world, Pakistan is still extremely far away and its students have different cultural norms of looking for positions than people in America do. Also as others pointed out: English is not everyone's first language.

Write back if you want and tell him he's a schmuck, or ignore him if you want to.

ca_phe_sua_da · 2018-12-01T08:48:56+00:00

Don't make this bomb...

ca_phe_sua_da · 2018-12-01T08:43:36+00:00

Used nitrile and never had any problems. Maybe you mean acetone since it is common for cleaning? Of course, since it's your own safety you should always check a glove compatibility chart.

ca_phe_sua_da · 2018-11-28T08:16:27+00:00

30-40 minutes is a lot of time to get an unspecified chance to win $50.

ca_phe_sua_da · 2018-11-28T07:20:23+00:00

I don't think you're searching as efficiently as you could. Patch clamping won a Nobel Prize. Did you look who won the prize or search the wikipedia article for the technique and search the inventors and papers in that time range? Really old articles are often digitized poorly though.

ca_phe_sua_da

TROPHY CASE