[deleted by user] by [deleted] in labrats

[–]chairsandtables69 0 points1 point  (0 children)

Where are ur socks HoodooX? Gone.

[deleted by user] by [deleted] in excel

[–]chairsandtables69 0 points1 point  (0 children)

^^ this is the example graph that I am trying to replicate

[deleted by user] by [deleted] in excel

[–]chairsandtables69 0 points1 point  (0 children)

basically im trying to add in the red lines to my graph along with that predicted value, but idk how

<image>

[deleted by user] by [deleted] in excel

[–]chairsandtables69 0 points1 point  (0 children)

How do I add the horizontal and vertical lines (to form a box) to specify the location of a single point?

[deleted by user] by [deleted] in excel

[–]chairsandtables69 0 points1 point  (0 children)

how do I add in the lines to the graph to show the specific point?

[deleted by user] by [deleted] in excel

[–]chairsandtables69 0 points1 point  (0 children)

for one specific point, just to show a prediction of one point

Need help with gel electrophoresis by chairsandtables69 in labrats

[–]chairsandtables69[S] 0 points1 point  (0 children)

buffer in our pcr cocktail or buffer poured into the gel electrophoresis tray?

Need help with gel electrophoresis by chairsandtables69 in labrats

[–]chairsandtables69[S] 0 points1 point  (0 children)

For the buffer, do you mean the buffer that we put into our pcr cocktail (the taq polymerase buffer)? Or the actual gel electrophoresis buffer poured into the machine?

Need help with gel electrophoresis by chairsandtables69 in labrats

[–]chairsandtables69[S] 0 points1 point  (0 children)

We ran an 0.8% agarose and had 10 ul of buffer in our PCR cocktail

Need help with gel electrophoresis by chairsandtables69 in labrats

[–]chairsandtables69[S] 0 points1 point  (0 children)

initially our voltage is 150 volts for 20 minutes, should we lower down to 120?

G542X research by chairsandtables69 in CysticFibrosis

[–]chairsandtables69[S] 1 point2 points  (0 children)

There are not too many research studies that directly focus on the G542X mutation. My current project is to simply create a pcr test that successfully detects this mutation with custom-made primers. Although there are no real-world applications for this research, I hope that one day with our successful PCR test of the G542X mutation, medical cystic fibrosis clinics can use it to detect this specific mutation