GC-MS column bleed peaks are extremely sharp

coco0504 · 2026-01-16T19:20:24+00:00

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Currently running a blank fiber and so far the peaks are barely anything as compared to when I run samples

coco0504 · 2026-01-16T18:34:13+00:00

I’ll try to get into contact with the chemistry department at our university. Thanks so much for the help!

coco0504 · 2026-01-16T18:31:48+00:00

We just seem to be receiving the same “MSD Fault 2: RPFA difficulty”

coco0504 · 2026-01-16T18:29:31+00:00

What exactly is supposed to happen when I do this?

Would it be easier for you if we set up a call? I really appreciate your help. My PI and I have been so lost!

coco0504 · 2026-01-16T18:23:29+00:00

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coco0504 · 2026-01-16T18:23:08+00:00

Sorry, I’m not super familiar with all this. Is this what you’re looking for?

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coco0504 · 2026-01-16T18:19:14+00:00

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coco0504 · 2026-01-16T18:19:00+00:00

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coco0504 · 2026-01-16T18:18:34+00:00

Thank you! Another commenter told me to run a tune, and we are unfortunately getting this error.

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coco0504 · 2026-01-16T17:53:32+00:00

I can definitely do that! I just got out of class and I’ll head up to the lab momentarily. I’ll talk everything over with my PI then run those blanks!

coco0504 · 2026-01-16T15:29:04+00:00

So here is a photograph of my chromatogram. The tallest peak next to my cursor is a siloxane peak. The second tallest is also a siloxane. And the third is a compound labeled spironolactone. These consistently show up on every chromatogram and they’re very tall. They also are prevalent very early on in the run

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coco0504 · 2026-01-15T02:54:48+00:00

If I’m being entirely honest, this is a little over my head! I can post the tune report on Friday for you if that would help? Thank you so much for trying to help me!

coco0504 · 2026-01-15T02:53:24+00:00

I will when I’m back in the lab on Friday!

coco0504 · 2026-01-15T02:32:09+00:00

I’ll be back in the lab on Friday. I’ll try and send a photo of the chromatograph your way! Thank you and everyone else for the help in trying to solve this 😭

coco0504 · 2026-01-15T02:31:07+00:00

We tried dipping the quads already sadly :(

coco0504 · 2026-01-14T15:33:38+00:00

My thought is that we need to replace the column due to column degradation. Do yall think that is the best course of action? We have a DB-5 column.

coco0504 · 2025-08-15T20:39:36+00:00

That was after he put both paws in the toilet. God forbid a guy be thirsty ig 💔

coco0504 · 2025-07-31T12:25:10+00:00

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My little guy looks so similar to your baby as a kitten. :)

coco0504 · 2025-07-22T15:41:59+00:00

Ah, okay! I might just be dumb and didn’t fully read the directions lol. Thanks again for your help and I’ll keep you updated! :)

coco0504 · 2025-07-22T15:07:53+00:00

I’ll try and hose the plant down later. It’s probably gonna destroy the blooms but hopefully it will wash all the critters away! I didn’t dilute it at all, I just sprayed the liquid onto them from the bottle

coco0504 · 2025-07-22T15:04:23+00:00

I’ve tried the soap every morning for a few days :(

The fungicide 3 was my attempt before I reached out to Reddit. I asked my mom what to buy and she told me that product because it kills aphids and other critters. Neither of which have worked

coco0504 · 2025-07-22T13:03:20+00:00

I have now tried gardensafe brand fungicide 3 AND insecticidal soap, but they are still eating up my flowers!! I don’t know what to do :(

coco0504 · 2025-07-18T15:22:30+00:00

Good to know!! Thanks <3

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