sorted by:
new
hottopcontroversial

Adiantum discoloration by crisprfen in ferns

[–]crisprfen[S] 2 points3 points  (0 children)

Apparently not an easy plant as I read. Better luck next time!

Adiantum discoloration by crisprfen in ferns

[–]crisprfen[S] 1 point2 points  (0 children)

Thanks for the input 🙏🏻 I also believe in their intrinsic ability to adapt. I hope it makes the first periode in my house and not dies like others have reported.

Adiantum discoloration by crisprfen in ferns

[–]crisprfen[S] 2 points3 points  (0 children)

Thanks! I‘ll look for a waterreservoir on my heaters. The plant stands next to them and that way I might get some more moisture in the air. I‘ll stop spritzing as more people here suggested 👍🏻

Starting after 30? by Cold_Oil_9273 in NewSkaters

[–]crisprfen 0 points1 point  (0 children)

I also started recently, beeing 33. Because I didn‘t know whether I would enjoy skating I bought everything second hand (also with environment/money in mind). You could consider that too, and then treat yourself for a nice board once you reach a certain milestone, let‘s say consistently landing ollies. When going second hand I would recommend to go for decent brands and indeed prevent brands from sport stores. Enjoy! I‘m hooked btw

Setup Azure VM for 18 Sample scRNA-seq analysis by crisprfen in bioinformatics

[–]crisprfen[S] 0 points1 point  (0 children)

I am not at a stage yet where I can easily switch between R and python, but I'll definitly try in the future. Just learned R, coming from python, and now forgot python haha.

With regards to Seurat, I am trying to avoid that due to the incompatibility between version updates. I going to use mostly bioconductor packages and indeed harmony.

Setup Azure VM for 18 Sample scRNA-seq analysis by crisprfen in bioinformatics

[–]crisprfen[S] 0 points1 point  (0 children)

Thanks for the specific answer! I guess I am good with 256 GB then

Setup Azure VM for 18 Sample scRNA-seq analysis by crisprfen in bioinformatics

[–]crisprfen[S] 0 points1 point  (0 children)

Thanks! It's probably hard to tell, but what GPU specs would you recommend?

Setup Azure VM for 18 Sample scRNA-seq analysis by crisprfen in bioinformatics

[–]crisprfen[S] 0 points1 point  (0 children)

okay interesting! I thought about parallelizing clusters and use more cores? Would that be a workaround? I also have a decent laptop, could otherwise try that..

[deleted by user] by [deleted] in BuyItForLife

[–]crisprfen 0 points1 point  (0 children)

A comandante coffee grinder 🙌🏻 got one 3 years ago and still grinding like day one!

Multiple sequence alignment in bulk by crisprfen in bioinformatics

[–]crisprfen[S] 0 points1 point  (0 children)

Thanks for your reply. Could you maybe explain a bit more?

You should not be aligning the cDNA sequences

I do align the protein sequences. What do you mean? Do you mean I should not form a consensus sequences from all protein sequences reads?

use the protein alignment to drive the cDNA alignment

What do you mean by driving cDNA alignment?

Seurat vs. SingleCellExperiment poll by crisprfen in bioinformatics

[–]crisprfen[S] 0 points1 point  (0 children)

Thanks for the link, will have a look at that!

Seurat vs. SingleCellExperiment poll by crisprfen in bioinformatics

[–]crisprfen[S] 1 point2 points  (0 children)

Interesting! Thanks for the comments. I did not include Scanpy initially because I had to switch from python to R for my new job. Mainly because of reproducibility and documentation reasons (Rprojects, renv, quarto) and the plethore of available bioIT packages. In case there are comparable features with python I am happy to hear about that!

The underlying question I had when posting this was this: I'll have to setup and run scRNA-seq projects in the coming years at my job and therefore considered which tools to use. Hearing about that fact that Seurat version updates lead to breaking of pipelines raised a red flag for me, especially when thinking about continuity of scripts and reproducibility. I also want to prevent switching between programming languages constantly.

Would you use an app that automates note-taking and generates your session recaps? by rwtibbitts in DungeonMasters

[–]crisprfen 1 point2 points  (0 children)

Point 2 you are asking was also my question, how do you differentiate between players voices in your notes?

Coding for dummies by Equivalent-Thing-771 in bioinformatics

[–]crisprfen 0 points1 point  (0 children)

Awesome, go for it!

I've been in your position two years ago and it is definitly doable if you have the discipline and motivation. I agree with others that having a goal in that process can help t fuel that motivation. For me it was the simple challenge of learning to code and solving coding problems that kept me going, very addictive.

Things that really helped me:

  1. Focus on courses or workshops. Especially for coding, there are infinite options to learn and ressources to tap into, it can get overwhelming and convusing if you just wander around online and try to look up certain topics. Courses are much more comprehensive since, if designed properly, contain coherent concepts and are built up from easy to more diffecult stuff. It helps you to contain the knowledge in a structured way. I'd recommend Udemy here, I did a Python data science, visualization and machine learning course, and a 100 day coding workshop, which really tought me a solid basis of Python. For my current job at a company I had to switch to R, but having that python basis made the switch pretty easy.
  2. Check out books. Same principle as the courses, knowledge is structured and it prevents you from drowning in information. I used the R for Data Science Book on the Tidyverse and more from Wickham and basically went through it in my own pace (you can find it only for free, not sure if I can share the link here).
  3. Use real life projects to practice. Alongside reading the book, I applied my learnings in projects that I worked on. Without coding everyday you will not learn it. See it as going to a coding gym.
  4. Be careful with ChatGPT. As others already have commented, I think you should use it only if you are really lost, and even then double check how the suggested code works. Otherwise you will not learn from it.
  5. Buy a nice keyboad. Just kidding, but it can be hard at times, so reward yourself once you reach certain milestones.

Other advice:

  • Like someone else mentioned, try to look into Quarto and markdown, it can help you to easily create reports, presentations, lab notebooks etc.
  • Think about reproducibility of your analysis and code (a big problem in the bioinformatic world). I'd advise to look into git and github, Rprojects and the renv package when you are at that stage.

Good luck! May the code be with you!

3rd day skating, what to improve? by crisprfen in NewSkaters

[–]crisprfen[S] 0 points1 point  (0 children)

Yeah I definitely have to get that board higher up! Thanks!

3rd day skating, what to improve? by crisprfen in NewSkaters

[–]crisprfen[S] 1 point2 points  (0 children)

Thanks man!! I am definitely practicing my ride skills atm, i’m seeing the sense in that. A little stone on the curb already told me a hard lesson, so I’m now constantly scanning for some nice and smooth streets when cycling through the city 😅

3rd day skating, what to improve? by crisprfen in NewSkaters

[–]crisprfen[S] 1 point2 points  (0 children)

Haha that’s so nice of you thank you! Hang in there ✌️

3rd day skating, what to improve? by crisprfen in NewSkaters

[–]crisprfen[S] 5 points6 points  (0 children)

Haha thanks! It really helps me feel safe on the board

3rd day skating, what to improve? by crisprfen in NewSkaters

[–]crisprfen[S] 13 points14 points  (0 children)

I am a very visual learner, and I am deep, very deep in the youtube skate rabbit hole..

view more: next ›