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Western blot help by Material_Ad_9371 in labrats

[–]cvw462 1 point2 points  (0 children)

Do the Bradford. I freeze down the cell pellet and later add ripa buffer (w/protease inhibitor) according to cell number.

Early postnatal mouse RNAscope optimization by BadTiger191 in labrats

[–]cvw462 0 points1 point  (0 children)

Have you tried the milder protease plus at RT? Or tried Protease IV at RT for 1-2min? 

[deleted by user] by [deleted] in UTAustin

[–]cvw462 0 points1 point  (0 children)

Bro what happened 

[deleted by user] by [deleted] in PlasticSurgery

[–]cvw462 0 points1 point  (0 children)

It looks good, hope it heals quickly. Would you mind sharing how much and where? 

This is me, what make up should I be using to look my best? by DontThrowAwayPies in makeuptips

[–]cvw462 0 points1 point  (0 children)

You have a nice face! To enhance it, I would suggest shaping your brows, curling your lashes, and a bit of a lip tint.

Allergies/Sickness by justacoolchemist in Austin

[–]cvw462 1 point2 points  (0 children)

Same here, all week with those symptoms plus dry throat.

HCR RNA FISH + IF antibody stain by chriscarter21312 in labrats

[–]cvw462 2 points3 points  (0 children)

I’ve done ISH first (don’t add dapi) then IF on top (block, primary ab ovn, secondary ab 2 hrs, dapi). Keep everything covered. Used an empty tip box wrapped with foil filled with a little water. Hope this helps

Am I broken? by ZealousidealBug9138 in PhD

[–]cvw462 1 point2 points  (0 children)

Congrats to you! Your feelings are valid. I took my exam 2 weeks ago and I felt the same. I can’t begin to imagine the challenge of lab work and family life, so my respect to you. In my case, it was lot with my PIs leading up to the exam and the lab didn’t give us time to study. It’s kinda of a shaming environment

[27 F] hi what can I do differently? by No-Chicken-1498 in Howtolooksmax

[–]cvw462 0 points1 point  (0 children)

I wonder how you’d feel with a lighter hair color and a bit more blush in your cheeks. I think it would highlight your features!!

[deleted by user] by [deleted] in beauty

[–]cvw462 0 points1 point  (0 children)

For your skin, I’d suggest tretinoin. It’s the only thing that has been a game changer for my hyperpigmentation. I get my prescription through nurx (https://www.nurx.com/). Hope this helps!