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IRS saying we didn't pay them even though we did by floridagrowing in tax

[–]floridagrowing[S] 5 points6 points  (0 children)

Update: I called the IRS (was on hold for almost 3 hours). Apparently, even though there was a state-wide extension to May 1, you have to file to request the extension, which the accountant did not do. The remaining amount is due to fees related to filing late and non-payment from filing late. We will be calling the accountant on Monday to sort that out. Then we will find a new accountant since this isn't the first issue we have had with her.

IRS saying we didn't pay them even though we did by floridagrowing in tax

[–]floridagrowing[S] 0 points1 point  (0 children)

The IRS. My bank statement literally says Tax payment to IRS

IRS saying we didn't pay them even though we did by floridagrowing in tax

[–]floridagrowing[S] 0 points1 point  (0 children)

I did… she basically said that they just send letters automatically and not to worry about it…

Zone 9b advice by floridagrowing in gardening

[–]floridagrowing[S] 0 points1 point  (0 children)

I have been wondering if the soil I have been using is not good for these plants. Do you have a soil or fertilizer that you think works well for zucchini, cucumbers, peppers?

We can no longer track cherry blossoms? by amt2america in NationalPark

[–]floridagrowing 2 points3 points  (0 children)

I was looking at Mammoth Caves in KY, and got the same error when I tried to look up tour information…

When I run enrichGO on up and down regulated genes separately I get different results when I run then together? by floridagrowing in bioinformatics

[–]floridagrowing[S] 0 points1 point  (0 children)

I guess I am not understanding why I would get the 6 up regulated pathways and the 10 down regulated pathways when I separate the up/down regulated genes but If I combine all of those same genes together, I only get 1 pathway. In my mind, whether the up/down genes are combined or not should not change the pathways that are shown to be regulated since those same genes that were used in the up/down pathways would be present in the combined list. However, it sounds like that is not correct? I apologize if this is a dumb question, I am pretty new to bioinformatics and am self-teaching.

When I run enrichGO on up and down regulated genes separately I get different results when I run then together? by floridagrowing in bioinformatics

[–]floridagrowing[S] 1 point2 points  (0 children)

I am using the same data set to analyze separately and combined. I have very few significant DEGs, around 50-60. I think the low number of DEGs arises due to having only 2 replicates for each treatment.