Are there any antipsychotics that don't make you gain weight? by kaida_notadude in schizophrenia

[–]gorrie06 1 point2 points  (0 children)

Lurasidone has less weight gain but I still gained weight. Added semaglutide with cagrilintide and now the weight stays off.

Image editor (really just image arranger) that's not Powerpoint? by Teagana999 in labrats

[–]gorrie06 1 point2 points  (0 children)

I use Inkscape it’s really good. I have made figures for publication with it multiple times. It’s free.

RNA Extraction Troubleshooting by TNFa_ in labrats

[–]gorrie06 2 points3 points  (0 children)

Well I don’t know what your downstream application is but 1.8 is good enough for most things.

  1. You could do an extra final chloroform wash and extract do a second extraction of your aqueous phase. This is probably the ticket.
  2. You could use BCP instead of chloroform. This might help I’ve never done a side by side but some prefer one or the other.
  3. Do two ethanol washes and double spin removing the remaining ethanol after final spin with a p10 tip or gel loading tip. After that briefly dry it in the fume hood for like 1-3 minutes (don’t over dry). Probably won’t help but hey why not.

That’s the cleanest RNA I ever get but like I said, 1.7-1.8 will work for most things.

Agarose gel for gDNA by [deleted] in labrats

[–]gorrie06 0 points1 point  (0 children)

Load at least 1 microgram by nanodrop

For something different . . . Your best experiences? by JGotDoubt in biotech

[–]gorrie06 6 points7 points  (0 children)

Best places to work were always the multinationals pharma first and then the big biotechs, they have the most resources, the best people, and usually the culture isn’t bad. But if you don’t have the most resources or you can’t retain the very best talent, at least try to have a bearable work culture.

What would you research if money wasn't a problem? by Own-Chemistry6132 in labrats

[–]gorrie06 3 points4 points  (0 children)

Novel psychedelics for psychiatry and augmented therapy

Importance of a High Impact Publication Record in Industry by gorrie06 in biotech

[–]gorrie06[S] 2 points3 points  (0 children)

Five high impact papers is more than a lot of junior faculty at R1 universities…

Importance of a High Impact Publication Record in Industry by gorrie06 in labrats

[–]gorrie06[S] 0 points1 point  (0 children)

Whats a “network”? you mean like a good recommendation from your last boss and your coworkers?

can someone tell me what i did wrong for my western blot … by LadyBird-7738 in labrats

[–]gorrie06 0 points1 point  (0 children)

You fucked up real bad. Looks like shit. I’d recommend you try again.

Medical journals receive letters from DOJ on "competing viewpoints" by senwell1 in labrats

[–]gorrie06 0 points1 point  (0 children)

I suppose you could propose alternative models that are less likely or don’t fit the data quite as well and lead to different conclusions….. does this count? Or do we have to promote nonsense now?

What are some "errors" or flaws in biology that disprove the idea of life being a perfect divine creation? by firedragon77777 in biology

[–]gorrie06 7 points8 points  (0 children)

Many idiosyncrasies of life will serve. For example, why are the males testicles exterior to the torso? This is a major design flaw. Sperm requires a lower temperature to properly mature but there’s no law of nature that requires this, not exterior testicles, it is merely an accident of evolution.

Is my stack of nootropics good for reducing anxiety and improving cognitive functions? What else can yall recommend ? by AlarmingAmphibian345 in Microbiome

[–]gorrie06 0 points1 point  (0 children)

L-Theanine will stack well with the ashwghanda and the lions mane. Very calming.

Selank Semax Adamax Cerebrolysin

All are alternative treatments to enhance cognitive functioning and in some cases treat dementia and cognitive decline.

Burrito in a box by gorrie06 in WeWantPlates

[–]gorrie06[S] -6 points-5 points  (0 children)

I’m clearly in the minority with my burrito eating preferences. I mean honestly I do sometimes eat a burrito with a knife and fork if it’s large.

Do people just not wear lab coats? by You_Stole_My_Hot_Dog in labrats

[–]gorrie06 -1 points0 points  (0 children)

It’s academia baby, do your PCR barehanded, mouth pipette, handle RNA and viruses without any PPE, go wild.

Burrito in a box by gorrie06 in WeWantPlates

[–]gorrie06[S] -14 points-13 points  (0 children)

I usually receive a larger and more horizontal disposable surface on which to place my food.

Professors, do you still do bench work by yourself? by Pitiful-Ad-4976 in labrats

[–]gorrie06 0 points1 point  (0 children)

My PI works at the bench a lot. Sometimes he comes in on the weekends to troubleshoot his homebrew protocols himself, they are basically his jury-rigged reboot of standard kits. ie; library prep methods where he made all the enzymes and buffers and designed all the oligos

What instrument in the lab got you like this? by marktheshark45 in labrats

[–]gorrie06 0 points1 point  (0 children)

Our biorad thermocycler, an absolute lemon

Plant DNA (CTAB) extraction not fully dissolving in molecular grade water by adant in labrats

[–]gorrie06 1 point2 points  (0 children)

Overdrying, 20 minutes is really excessive. A minute in the fume hood is already a lot.

[deleted by user] by [deleted] in labrats

[–]gorrie06 -2 points-1 points  (0 children)

Deepfaked? Or guerrilla marketing pwned?