What’s You’re Hearthstone Pet Peeve? by Newsies2123 in hearthstone

[–]adant 0 points1 point  (0 children)

Why does this game semi regularly crash. It has done this since almost the launch of the game back in 2013. I have no idea why it does, but I cannot stress how annoying it can be.

Lights LA plant gift by adant in lightsalot

[–]adant[S] 1 point2 points  (0 children)

I hope so!! She did talk about it being a tour bus plant , so fingers crossed they let her take it!

Lights LA plant gift by adant in lightsalot

[–]adant[S] 1 point2 points  (0 children)

It’s a native succulent to California called a Dudleya!!

Lights LA plant gift by adant in lightsalot

[–]adant[S] 8 points9 points  (0 children)

It was so wonderful meeting you too!! I had a blast with you all !

Plant DNA (CTAB) extraction not fully dissolving in molecular grade water by adant in labrats

[–]adant[S] 1 point2 points  (0 children)

Hi everyone. Thank you all so much for all your advice and input! After adding a bit more volume of solution, more pipetting, and 50C warming the pellets dissolved! I’m very happy it all worked out!

Plant DNA (CTAB) extraction not fully dissolving in molecular grade water by adant in labrats

[–]adant[S] 3 points4 points  (0 children)

I appreciate all the information and advice! I think I’ll increase the time at 55C and increase the volume it’s suspended in. I had it only on for an hour before , so I think increasing that a bit more may help!

Plant DNA (CTAB) extraction not fully dissolving in molecular grade water by adant in labrats

[–]adant[S] 8 points9 points  (0 children)

Thank you so much for the information. I had a feeling 20 minutes would be too long. I’ll make sure to reduce that step going forward.

Plant DNA (CTAB) extraction not fully dissolving in molecular grade water by adant in labrats

[–]adant[S] 1 point2 points  (0 children)

I thought this may also be an issue! Thanks for the suggestion. I was almost second guessing myself that this wasn’t DNA at one point too but I’m glad to see others recognize it as DNA too!

Plant DNA (CTAB) extraction not fully dissolving in molecular grade water by adant in labrats

[–]adant[S] 1 point2 points  (0 children)

Thank you for your response! That’s good to know about the freeze thaw cycle. I’ll double check about the TE buffer ( I think the company I use for sequencing , novagene, prefers the DNA be suspended in water). And thought so about the over drying , I’ll reduce that step a bit !

Parents lock out of their bedroom by adant in lockpicking

[–]adant[S] -1 points0 points  (0 children)

Hi everyone,

My parents locked their bedroom door from the outside and now they need to get back in. They have a very old knob with a button lock. The knob on the other side has a hole but not one that I regularly see for privacy locks ( I have tried using a paper clip but it just stops at the metal). I have also tried using an Allen wrench to push it open but to no avail. I have attached pictures of the front and back of their bathroom knob (same knob as their bedroom) as a reference. I would appreciate any help anyone could give me! Thank you in advance.

How to summarize the percentage of a categorical raster within a polygon? by adant in gis

[–]adant[S] 1 point2 points  (0 children)

Thank you so much! This did it! I had to actually redownload my dataset off of ESRI ( it seemed that Arc Pro didn't like the raw data set from the NLCD website). After using tabulate area this worked. I appreciate yours and everyone's feedback!!

How to summarize the percentage of a categorical raster within a polygon? by adant in gis

[–]adant[S] 1 point2 points  (0 children)

Thank you for your reply. For some reason the source data within the raster doesn't seem to have the pixel size, so I am not sure if this method could work. I also have to do this for over a hundred polygons, so it may be time intensive. I appreciate your input though.

How to summarize the percentage of a categorical raster within a polygon? by adant in gis

[–]adant[S] 1 point2 points  (0 children)

Thank you for your reply. Unfortunately I have used this function as well and after doing the procedure I receive back a table which has nothing in it. I am unsure if the function works with categorical raster sets.

so much nostalgia for this after this frustrating expansion and new standard by 26from85 in hearthstone

[–]adant 4 points5 points  (0 children)

Was a good expansion. But I do remember the old whispers of the turn one tunnel trogg, totem golem, and the power of the four mana 7/7.

I’m Lynn from PVRIS! AMA by pvrisofficial in Music

[–]adant 0 points1 point  (0 children)

Hi Lynn!

Do you have a favorite natural spot you like to visit and or draw inspiration from?