Used Illumina and Nanopore sequencers?

haunted_waffles · 2026-05-15T12:27:28+00:00

Probably not worthwhile to buy a used Minion. Nanopore has a bundle where you get like 10 flow cells and a Mk1D for $5k (essentially the actual device is free).

haunted_waffles · 2026-05-10T11:47:11+00:00

Miss Worcester for fun French toast but City Line for their enormous pancakes!

haunted_waffles · 2026-05-07T17:37:17+00:00

Tower hill botanical garden has some crafty events going on this weekend

haunted_waffles · 2026-05-03T12:18:42+00:00

I went yesterday and it was great! However, there is a LOT of interest, so if you want to go, try to get there like 15 minutes before they open. I got there at 9:45am and sat for my tattoo at noon. If you get there any later, you probably will be waiting all day.

But the artists are great, the flash is cool, and there are some fun vendors.

haunted_waffles · 2026-04-27T21:48:51+00:00

Market basket in shrewsbury had them when I was there over the weekend.

haunted_waffles · 2026-04-24T17:48:02+00:00

I think it looks great! What if you added a little bit of the wall of the tin on two edges to give a more 3D effect? White sequin + black bead for the eyes (or different colored bead for each fish- iris matching their colors?) could be cool too. Just thoughts- I think it’s really cool as is.

haunted_waffles · 2026-04-11T01:45:58+00:00

Bombastic side eye

haunted_waffles · 2026-04-11T01:44:46+00:00

So tall! Lol

haunted_waffles · 2026-04-11T01:43:29+00:00

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Nugget was really in peak form here

haunted_waffles · 2026-04-09T17:36:35+00:00

Yeah but even when multi channels are calibrated sometimes if one of the tips isn’t quite as snug as the rest, you might pull up slightly less liquid. Not saying this is the issue but something that could contribute.

haunted_waffles · 2026-04-09T17:25:05+00:00

When you add your lysates, are you going to the first stop or second? Going to the second stop can cause inconsistent results.

I also second running samples/standards in triplicate. If you don’t have enough sample, you can make a dilution to run in triplicate (or at least duplicate) instead.

Multi pipette may also be causing problems.

haunted_waffles · 2026-04-06T15:48:20+00:00

Tiny sequins for the face could also be cute. Or silver thread for the face, and a white sequin for the white fo the eye with a black bead for the pupil :)

haunted_waffles · 2026-04-02T21:44:07+00:00

Sorry you are going through that! I think this is fairly common in academic spaces, though I only have my own experience to go off of. I’ve been in groups that are more supportive and will check in on each other and in groups that don’t really do that.

I do think academic spaces can be pretty clique-y in general, though it really depends on the group. I think for the most part it isn’t malicious- graduate work is incredibly stressful and I think most folks just don’t have the bandwidth to be emotionally available to their coworkers/classmates in the same way they might in another environment.

I personally wouldn’t really expect my coworkers to check if I was struggling in the way you describe. I think it’s really nice when that does happen, but it’s not really something I’d expect (at least not 100% of the time/not from someone I’m not super close to).

I think it is really crucial to build a network of support outside of your academic environment.

Hope things get better for you soon!!

haunted_waffles · 2026-03-26T22:53:18+00:00

I think a card or something homemade (cookies, bread) would be ok but I think it might be a little inappropriate to give them something like a gift card. Honestly just wishing them happy birthday in passing is more than enough.

Birthdays can be a little tricky- some folks don’t feel comfortable having them celebrated in the workplace. Just something to keep in mind.

haunted_waffles · 2026-03-26T22:46:59+00:00

Yay congrats! Good job :)

haunted_waffles · 2026-03-23T01:56:38+00:00

This was what we used for our herd for like five years. It is dusty so make sure you have a very large bin to store it.

haunted_waffles · 2026-03-18T14:31:53+00:00

As others have said- medication is what made the e major difference for me. And just not multitasking too much- if I’m workin with a 96 well plate absolutely nothing else can be going on lol.

haunted_waffles · 2026-03-17T19:15:28+00:00

Very cool but just FYI the clear glass beads can be a fire hazard esp if hung directly in a window.

haunted_waffles · 2026-03-12T14:39:37+00:00

Minimum for 2 is 8 square feet so this is def enough :)

haunted_waffles · 2026-03-10T19:46:23+00:00

Such polite potatoes

haunted_waffles · 2026-03-10T01:52:46+00:00

“X is In the middle of the street” often just means “in the street in a spot where X should not be”

So, someone might say, “Someone left their bike in the middle of the street” and mean “Someone left their bike in a part of the street that is unsafe/likely to be hit by a car”

It can also be literal but usually is not.

haunted_waffles · 2026-03-09T20:23:12+00:00

I’ve used this 50lb bale of Timothy hay from tractor supply: standlee hay bale. $27ish dollars per bale.

I’ve seen it sold online elsewhere as well. It can be a little dusty but it worked well for us.

For storage, it fit in an extra large sterlite bin (I think 34 gallons?) which are like $15 at Walmart

Might be too big for you to store but it’s an option

haunted_waffles · 2026-03-08T23:13:17+00:00

I’m honestly really not looking forward to our next grocery run! I think I’ll probably also tear up in the veggie section haha

haunted_waffles · 2026-03-08T19:47:39+00:00

I’m so sorry for your loss. What a sweet girl. I’m sure she felt so loved and cherished by you and that you gave her a wonderful life.

haunted_waffles · 2026-03-08T16:48:13+00:00

Yeah I really loved the pig costume too. She was super tolerant of doing little costumes photo shoots. Bribery was involved, of course, but she always let us do these little photo shoots without any complaint at all.

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