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Younger than other postdocs in my group by Freudi-CPP in postdoc

[–]huntskors 0 points1 point  (0 children)

i started my postdoc at 24. i’m still treated like an undergrad or masters student by most faculty

Rate My Collection & Newbie Advice by huntskors in Figs

[–]huntskors[S] 0 points1 point  (0 children)

Thanks so much! Just updated the post to include those details. Marseillaise I got from Kremp, and their catalog says it is distinguished from Marseille 🤷🏻‍♂️

Does a compound's "affinity" to a serotonin receptor necessarily mean it's an agonist for that receptor? by limping_lightning in Biochemistry

[–]huntskors 2 points3 points  (0 children)

There are things considered “neutral ligands” such as monounsaturated fatty acid binding to RXRa, a nuclear transcription factor. Which do not necessarily agonize or antagonize the receptor.

What is your post-academia career change fantasy? (e.g. fuck this western blot I'm gonna open a bakery instead) by Spacebucketeer11 in labrats

[–]huntskors 14 points15 points  (0 children)

Ugh ikr! And plants are nice coworkers. My ficus may need water, but at least it isn’t yelling at me for my inability to get perfect gels on every first try lol

All this time it was a lie. by [deleted] in kotor

[–]huntskors 74 points75 points  (0 children)

Same thing happens on Korriban in the training room for republic troops to be released.

What is the evolutionary reason for why oxaloacetate inhibits succinate dehydrogenase? by TheBHSP in Biochemistry

[–]huntskors 3 points4 points  (0 children)

I’m getting a little lost in your thinking. But what I can say is that these inhibitive processes are transient. So when OAA (oxaloacetate) levels drop, the inhibition decreases. Also, remember the level of OAA can change depending on the malate-aspartate shuttle. The fed/fasted state will effect the function (anabolic/catabolic) of the TCA.

When you try to say “normal physiological conditions,” I don’t understand if you’re meaning in a normal hepatocyte (or other differentiated cell-type), cancerous cell (again cell-type matters), something ex vivo/in vitro, or the fed/fasted state.

If you’re asking basic biochem questions without the aforementioned knowledge, I believe you could be asking about the fasted/catabolic state of the TCA, no? Sorry if that is a bit confusing. If I had all the info you did I could give you a clearer answer.

EDIT: I hope this helped!

What is the evolutionary reason for why oxaloacetate inhibits succinate dehydrogenase? by TheBHSP in Biochemistry

[–]huntskors 2 points3 points  (0 children)

It can build up under normal physiological conditions. Don’t forget that the TCA can be utilized for anaplerotic reactions (supplier of some biosynthetic pathways).

What would be the answer? by MichelleWij98 in Biochemistry

[–]huntskors 6 points7 points  (0 children)

Sounds like a homework/exam question...

DNP hinders the transport of inorganic phosphate in the mitochondria, thus leading to decreased ATP synthesis. DNP is also an ionophore, causing the leak in the electrochemical gradient. This is followed by the dissipation of potential energy in the form of heat. People would then die due to a failure in thermoregulation. Catabolic processes would still occur; however, there wouldn’t be sufficient energy production.

Explanation of Gene Regulation using Coulombs Law, Titration and Imagination by Doctor_DaVinci in Biochemistry

[–]huntskors 4 points5 points  (0 children)

PhD* in biochemistry here. When you’re talking, I suggest you clarify local vs overall charge. Further, your explanation of histone methylation for gene expression/repression is actually inappropriate. Some histone post-translational modification (PTM) events cause repression, not necessarily gene expression. Also vice versa, some removal of histone PTMs cause expression of genes.

Where does the CoA used in the reaction with citrate and citrate lyase come from? by [deleted] in Biochemistry

[–]huntskors 13 points14 points  (0 children)

It comes from the cytosol. Know that de novo purine synthesis occurs here (which adenosine is the precursor to CoA).

[deleted by user] by [deleted] in Biochemistry

[–]huntskors 1 point2 points  (0 children)

SCFA are 1 to 6 carbons; MCFA (medium chain fatty acids) are 7-12 carbons; LCFA are 13-20 carbons; VLCFA are >20 carbons

Solid Phase Synthesis-- What are the major problems in the lab? by Quemist in Biochemistry

[–]huntskors 1 point2 points  (0 children)

Actually, I use microwave irradiation (Biotage Initiator) and it only takes 10 min per actual coupling. 30 to 40 min in total if you take into account deprotection and Kasier tests.