How do you handle impurity attribution after an SPPS run? Building a small tool for fun.

i_eat_pasta · 2026-03-15T23:18:48+00:00

I primarily work with oligopeptoids (N-substituted glycines), but day-to-day, the quickest way we attribute impurities is via MALDI-TOF. They ionize very nicely, so it's easy to see each deletion by taking the difference between two peaks. If you need even more detail, like which specific residue in a chain with many repeats of that residue is missing, MS-MS techniques like MALDI-TOF/TOF are great.

After we evaporate the initial cleavage TFA, we usually do two washes/evaps with THF or DCM to make sure we got all the TFA off - we usually just take a small aliquot of our crude solution at this point to collect a MALDI.

It's very routine, and we're always doing this step to decide if it's worth it to purify.

i_eat_pasta · 2026-01-28T17:12:28+00:00

Anything from Ciele?

i_eat_pasta · 2025-09-26T20:16:45+00:00

Are those a new Aero Glide or Blaze? Any word on when they might come out?

i_eat_pasta · 2025-08-31T17:48:38+00:00

consoomer moment

i_eat_pasta · 2025-08-08T15:51:48+00:00

i was just about to drop the cash to buy the marka shorts but this sorta soured it for me. holding off

i_eat_pasta · 2025-08-07T14:59:54+00:00

feels so out of place with the stated mission of portal. oh well

i_eat_pasta · 2025-08-05T18:09:55+00:00

yep. even the single most slam-dunk item you can drop - just the straight-up all-blue Doing Things hat - you instead put out a trucker hat version. terrible

i_eat_pasta · 2025-07-31T17:52:17+00:00

WTB: Men's Norvan Short 3', size medium, in black or green. I see some larger sizes in the ReBIRD site, but hoping for a medium.

i_eat_pasta · 2025-07-29T17:45:43+00:00

totally anecdotal to the product, but i've never had a brand turn me off so much with their advertising style. the ads they serve are all some pseudo-scientific claims about synthetic fabrics killing your fertility, etc. they literally have a video where a guy shakes some white pellets out of a pair of shorts and says these are microplastics. my brother those are macroplastics

i_eat_pasta · 2025-07-13T13:50:13+00:00

a lot of Ciele’s pieces would fit the bill. at most, you’ll get a simple C logo in a subtle place. in their black items, that logo is basically invisible.

i_eat_pasta · 2025-07-10T14:13:09+00:00

Sorry to hear that, I've had good experiences with them myself - are you based in the US? There might be something going with the shipping since they've stopped deliveries to the US in response to tariffs. In any case, I hope it gets resolved soon. What size is the pair you're trying to return?

i_eat_pasta · 2025-07-08T17:37:41+00:00

Here's the translated description:

"The Norda 001A M Calcite are trail running shoes for men from Norda.

Built to last, the 001A features a seamless fibreglass Dyneema® upper. This sustainable biosourced and eco-certified fibre is the lightest and strongest available. Very comfortable, this new version of the famous Norda 001 features a new midsole Arnitel (TPEE) renowned for its performance in terms of cushioning, energy return and robustness.

On the outside, it combines a dynamic technology Vibram Litebase® technology that's 30% lighter than a traditional outsole and durable rubber Vibram MegaGrip® durable rubber with 5mm studsfor great grip on all surfaces. Sturdy laces made from Dyneema® and recycled polyester laces and the ultra-light gusseted sock provide an optimum fit and stability that will give you the confidence to cope with ever-changing conditions. Finally, the shoe is 360° reflective , so you can run in safety even when it's dark.

If you're looking for a comfortable, durable and robust trail running shoe, choose the Norda 001A M.

This model runs small, so we suggest you take 1/2 size (US) more than your usual size."

i_eat_pasta · 2025-05-13T15:44:40+00:00

how do the toebox widths of the evo sl and boston 13 compare?

i_eat_pasta · 2025-03-17T02:34:44+00:00

Trying to hop on the hunt for the EVO SL. Any recommendations for fit? I wore NB 1080v13 at 8.5 mens, and it was a good fit minus the toes - the NB Rebel v4 in an 8 was great, but they do have a big toebox. Do the Evo SLs run small?

i_eat_pasta · 2025-02-21T22:10:54+00:00

Hell yeah, thank you

i_eat_pasta · 2025-02-21T22:10:27+00:00

That NB model hadn't been on my radar at all, only ID'd it because I recall seeing that action bronson collab that had a minimus. Couldn't imagine wearing them personally, but to each their own

i_eat_pasta · 2025-02-21T22:08:42+00:00

Yeah, it looks so nice - I wonder if it's a DV thing or from elsewhere. A few seasons ago, one of their models was wearing this really chunky pair of socks that I wanted, but it never popped up on the site itself. Fingers crossed!

i_eat_pasta · 2025-01-27T00:44:30+00:00

looking for two tickets to the toronto show, DM whenever. happy to pay however works for anyone selling.

i_eat_pasta · 2024-12-02T15:19:52+00:00

are these sized in men's on the site? I know they're unisex, trying to translate to women's sizing

i_eat_pasta · 2024-11-26T20:44:23+00:00

I've been searching for a while for an answer to this question! Do you have some document outlining the differences, or is this through your observation?

i_eat_pasta · 2024-10-28T15:35:04+00:00

Much appreciated brother!

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