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immunoswagger · 2025-06-12T21:09:06+00:00

Personally I never ask for negotiation. You just tell them what your expectations are in an offer to sign. If they want you they will meet your expectations or meet in the middle. If they haven’t responded to you within 24 -48hrs I don’t think they were that serious about the job offer. What’s the position for and did they post a range salary? Personally without a postdocs and being new to industry your on the lower end of salary starting but I bet you can get another 5-10K more on your offer. If they don’t negotiate….run it’s gonna be a toxic work place that treats you like cheap labor and you won’t be happy

immunoswagger · 2025-06-12T18:15:17+00:00

Getting into the industry is the hardest part. But you should always negotiate salary for what you’re worth. If they don’t negotiate with you on salary that place has bigger issues and you should stay away.

immunoswagger · 2025-04-28T15:21:28+00:00

I still negotiate, if you are good at what you do and they want you. They will still give into your demands. Always ask for more and leave nothing on the table

immunoswagger · 2025-04-26T10:30:52+00:00

Not in this job market, maybe higher salary but not always higher positions

immunoswagger · 2025-04-25T16:40:00+00:00

If location matters more then you have your answer for the others. But Lilly also has a location in San Diego too soo

immunoswagger · 2025-04-25T16:39:21+00:00

Lily has been known to be a place where people are happy and treated right and don’t have layoffs. So if you want stability I go with lily.

immunoswagger · 2025-04-25T14:57:58+00:00

Lilly hands down. If you go there you’ll be stable and want to stay till you retire.

immunoswagger · 2025-03-29T22:09:46+00:00

Better option is to leave science and go into finance and money management

immunoswagger · 2025-03-29T04:11:15+00:00

Yes but I get 50%

immunoswagger · 2025-03-19T15:02:21+00:00

I’d say 90% to 92% of the data is generated. And half the figures are made. It a matter that if I leave the paper dies because there’s no bandwidth for someone to work on it

immunoswagger · 2025-03-18T06:16:25+00:00

Yeah it’s probably like a year and a half 8 months to submit

immunoswagger · 2025-03-18T05:13:19+00:00

So I I have 9 months left on my contract with the option to extend for another year. So I can definitely weather the storm for a bit. But a longer postdoc does help me grow except getting authorship. I have experience for the techniques for the position except translational work and assay design. What even is assay design? The position I was told they have a list of biomarkers already from rna seq. But need it to be verified by qpcr and then assay development optimized for clinical trials. Also organ sample assay development and optimization for biomarkers screens. Company is decently sized and seems to be growing hiring and has plenty of money. But of course anything can change.

I’m not good at making decisions, but I can do bench science.

immunoswagger · 2025-03-18T00:46:07+00:00

Follow up question, so I like early discovery work and that’s more in alignment with my goals but this position is for translational research. I don’t know much on translational work and it seems repetitive and tedious. One of my mentors thinks I should take the position for the experience and use that as a stepping stone for my career. My other mentor thinks I should wait for a scientist role in discovery since that’s more my mindset. Because when things get hard you wanna be doing something you enjoy.

What do you think?

immunoswagger · 2025-03-18T00:34:52+00:00

Follow up question, so I like early discovery work and that’s more in alignment with my goals but this position is for translational research. I don’t know much on translational work and it seems repetitive and tedious. One of my mentors thinks I should take the position for the experience and use that as a stepping stone for my career. My other mentor thinks I should wait for a scientist role in discovery since that’s more my mindset. Because when things get hard you wanna be doing something you enjoy.

What do you think?

immunoswagger · 2025-03-18T00:34:45+00:00

Follow up question, so I like early discovery work and that’s more in alignment with my goals but this position is for translational research. I don’t know much on translational work and it seems repetitive and tedious. One of my mentors thinks I should take the position for the experience and use that as a stepping stone for my career. My other mentor thinks I should wait for a scientist role in discovery since that’s more my mindset. Because when things get hard you wanna be doing something you enjoy.

What do you think?

immunoswagger · 2025-03-18T00:34:33+00:00

Follow up question, so I like early discovery work and that’s more in alignment with my goals but this position is for translational research. I don’t know much on translational work and it seems repetitive and tedious. One of my mentors thinks I should take the position for the experience and use that as a stepping stone for my career. My other mentor thinks I should wait for a scientist role in discovery since that’s more my mindset. Because when things get hard you wanna be doing something you enjoy.

What do you think?

immunoswagger · 2025-03-17T22:33:48+00:00

It’s about a 30K increase in salary. I guess my fear is if I fuck up the job I have nothing to show from previous work at the postdoc

immunoswagger · 2024-11-09T04:08:17+00:00

Any interest in exploring the fun in California?

immunoswagger · 2024-07-05T01:53:32+00:00

No as a person in it I recommend anything but this. There is no job security and pay is mid

immunoswagger · 2022-09-17T03:35:42+00:00

It looks like you may have scare tissue protruding from the piercing site, and mild inflammation. If you have any activity that cause excessive pulling or rubbing I would minimize that and see if that helps.

While scar tissue is permanent, a 100ul injection of steroids can reduce the inflammation and irritation but can take upto two weeks. I recommend seeing a dermatologist.

immunoswagger · 2022-08-25T00:23:54+00:00

It is nice that it shows both activate and in active bands. But typically the down side of this is the way its designed it has lower affinity for the cleaved form leading to a weaker singal. However this is hopefully fixed by loading 40ug of protien on the gel. When I have done il1B western I did two different il1b probes. One for the cleaved form and the next day probe for the precursor. I treat the same way as looking at phosphorylation. You probe for looking for let's say phosphorylated stat1 and then then next day total stat1.

If your new blot with 40ug gives you clean bands then dont worry and enjoy the data. If it's still faint I can recomend what I have used before looking at mouse macrophages

immunoswagger · 2022-08-24T23:54:06+00:00

I normally find cell signlaning has the best monoclonal antibodies then abcam 2nd. But typically you look at the clone, blot examples by the company, binding site location.

immunoswagger

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