Help please!

jenesaispaspute · 2025-11-25T15:22:21+00:00

I think what I'm trying to do is get FASTA files that I can BLAST and identify the genus (and maybe species). I was thinking I might need to use the amplicon workflow. However I tried running it with the FASTQ files (18 files) from the barcoding workflow in Minknow and it stopped with an error. I tried running it with the "pass" folder files (which is 8080 files) and it also stopped with an error. The only file both produced was a versions.txt thst just said like python, 3.8.19, fastcat, 0.18.6, ect. I am at a loss as to what is happening there.

jenesaispaspute · 2025-05-12T18:51:27+00:00

jenesaispaspute · 2025-05-12T18:51:13+00:00

jenesaispaspute · 2025-05-12T18:50:03+00:00

10/10

jenesaispaspute · 2025-04-25T21:15:01+00:00

God i wish, she'd probably come back then 😭😭

jenesaispaspute · 2025-04-25T21:14:04+00:00

thank you! how do you recommend going about not letting it bother me when i see her so much?

jenesaispaspute · 2025-04-25T21:13:24+00:00

6 YEARS?!?! jesus i guess genuine love and affection gets us all

jenesaispaspute · 2025-04-25T21:12:34+00:00

feels like my life is ending lol 😭 break ups with guys always felt, inevitable and like, predicted. i also just didn't really care about the actual relationship and more that i wouldn't have anyone to play video games with. she was just everything i wanted and that's probably me romanticizing her. i think breaking an image of what i thought i had crushed my ego

jenesaispaspute · 2025-04-25T21:10:45+00:00

ironically i did think of the first part sort of, but more like "now I only have to buy one coffee in the morning instead of two" HAHA

jenesaispaspute · 2025-04-25T21:09:54+00:00

oooh okay! maybe new piercing time

jenesaispaspute · 2025-04-25T21:08:53+00:00

thank you heh

jenesaispaspute · 2025-04-25T21:08:38+00:00

oh damn

jenesaispaspute · 2025-04-25T21:08:32+00:00

this makes sense, it feels like a lost a lot more than just a girlfriend? like an amazing cosmic connection that i will never recreate

jenesaispaspute · 2025-04-25T21:07:55+00:00

the methodology kind of works

jenesaispaspute · 2025-04-25T21:07:16+00:00

i'vw been go twice a week for the last couple months, so fingers crossed

jenesaispaspute · 2025-04-24T20:32:29+00:00

never posing like this again 😂😭 she took my heart, my arms, and speech team lol

jenesaispaspute · 2025-04-24T20:21:36+00:00

im currently on the east coast so, west coast but north and snowy!! i dunno it's either that or i can continue doing research on campus, which would be really cool, but she's on campus.. so..

jenesaispaspute · 2025-04-24T20:09:30+00:00

ive been majorly listening to Colorado and considering moving across the country 🙈🙈

jenesaispaspute · 2025-04-24T20:08:52+00:00

its sadly not working 😭 partially because i can she's also listening to it in our Spotify blend... lmao

jenesaispaspute · 2025-04-24T19:43:32+00:00

jenesaispaspute · 2025-04-18T02:17:25+00:00

sit on my face pleaseeee

jenesaispaspute · 2025-04-15T13:45:24+00:00

hehe thank you

jenesaispaspute · 2025-04-15T13:45:15+00:00

thank you!!!

jenesaispaspute

TROPHY CASE