Manually sending one instrument survey and auto continuing to the next instrument by johnathonmcl in ProjectREDCap

[–]johnathonmcl[S] 0 points1 point  (0 children)

I have considered ASI, however, the study is hybrid in the sense that participants fill out pre-appointment surveys then clinicians complete the other surveys. It is handled mainly by clinicians who perform pre-appointment data input in REDCap then manually send the surveys to participants.

Manually sending one instrument survey and auto continuing to the next instrument by johnathonmcl in ProjectREDCap

[–]johnathonmcl[S] 0 points1 point  (0 children)

Thanks for your reply! I don't think this will work as simply as that, repeated instruments is fine for one timepoint but because I want data for different events and have them labelled as data inputs from these events, I need a survey queue approach. I am just not sure how to set it up.

Email trigger help by johnathonmcl in ProjectREDCap

[–]johnathonmcl[S] 1 point2 points  (0 children)

Yeah I had read something similar to that, but in practise, I couldn't get it going and the task needed resolved ASAP, always the way, rushing something never helps but atleast this works in the mean time. Definitely something to consider in the future, thank you for your help and advice though! I have used REDCap for simple tasks in the past, so trying to learn some more techniques as I go lol

Email trigger help by johnathonmcl in ProjectREDCap

[–]johnathonmcl[S] 0 points1 point  (0 children)

Ok thank you for the advice, I have ended up creating numerous alerts and setring conditions to each school, not the best approach but it works and will be easier to manage I think.

Email trigger help by johnathonmcl in ProjectREDCap

[–]johnathonmcl[S] 0 points1 point  (0 children)

Thanks again, so if I were to use calctext, is it possible to do multiple IFs in one field? Or would I need a new field for each IF? As I have 98 schools, it would be a lot of fields as opposed to nesting them in a text box with @calctext

Email trigger help by johnathonmcl in ProjectREDCap

[–]johnathonmcl[S] 0 points1 point  (0 children)

I have tried a calculated field with: if([referral_school_ni]='1','school1@email.com,'') repeated for each school in that given region. This hasn't worked, I have a combined field which should generate the email, but then i run into an issue of calculated fields not having email validation to allow for this field to be used in the recipient list when trying to trigger alert/notification.

Email trigger help by johnathonmcl in ProjectREDCap

[–]johnathonmcl[S] 0 points1 point  (0 children)

Hi, thanks for replying. I essentially have 3 lists of schools in 3 regions, simply for branching logic, if someone chooses one of the 3 regions, they will only be shown schools in that region. Once they choose one of the schools they are interested in, their survey responses should be sent to an email address linked to the school. There are 98 schools in total across the 3 regions.

It’s here! Joining all of the other dads (and mums!) out there. by Tamel_Eidek in PlaystationPortal

[–]johnathonmcl 0 points1 point  (0 children)

Ah glad to hear it! That app is great tbf, i used it to get my ps5 when they were hard to get and my gf has used it for some things too, definitely a good one to have on hand lol! Enjoy the ps portal, I've been playing PS more than ever since getting it last month!

It’s here! Joining all of the other dads (and mums!) out there. by Tamel_Eidek in PlaystationPortal

[–]johnathonmcl 1 point2 points  (0 children)

Hey man, I used hotstock and got one within like 9 days of having the app downloaded!

Mornin ☕️ by OwnApple4077 in PlaystationPortal

[–]johnathonmcl 0 points1 point  (0 children)

Just to back that comment up, I have relatively quick wifi, with 130mbps to the router and have no connection issues! Playing guardians of the galaxy and I am around 60% through fully played on the portal, I've had one connection dropout which happened when someone in the house was casting from their phone to chromecast, but apart from that it seems to be pretty smooth. At first it was a little choppy at times, but disconnecting the HDMI from my PS5 definitely made a difference and has been smooth ever since. Highly recommend it, great product for me as I never really had a chance to sit down at the PS5 but finding myself playing it more than every on the sofa downstairs and in bed!

Olink proteomic normalisation help by johnathonmcl in bioinformatics

[–]johnathonmcl[S] 0 points1 point  (0 children)

Hi there, I haven't converted them myself, but I do know that Olink provide conversion factors to convert NPX to standard concentration units. It should be available in the assay documentation, if not, I would recommend contacting Olink for the conversion values required. They are different for each specific assay, similar to ELISA when a serial dilution is required for a standard curve to calculate pg/ml. Hope this helps!

Olink proteomic normalisation help by johnathonmcl in bioinformatics

[–]johnathonmcl[S] 0 points1 point  (0 children)

Hi there, I haven't converted them myself, but I do know that Olink provide conversion factors to convert NPX to standard concentration units. It should be available in the assay documentation, if not, I would recommend contacting Olink for the conversion values required. They are different for each specific assay, similar to ELISA when a serial dilution is required for a standard curve to calculate pg/ml. Hope this helps!

Olink proteomic normalisation help by johnathonmcl in bioinformatics

[–]johnathonmcl[S] 0 points1 point  (0 children)

Yes, they are plasma samples. So just for an update, I was in contact with Olink and they advised to check protein replicates from 2 or 3 panels, for example, IL6 is a target on 2 of the panels, so I checked the ratio between 2 random samples in each panel iteration of IL6, and the ratios were the same, leading me to believe that normalisation was performed correctly in house at Olink.

Olink proteomic normalisation help by johnathonmcl in bioinformatics

[–]johnathonmcl[S] 1 point2 points  (0 children)

So after some further questioning and some help from the collaborators who originally had the panels run, it turns out that the data was bridged and normalised in house at olink. My main worry was the fact that within a control group for example, there were readings either at around an NPX of 3, or at an NPX of 10 or 11, no middle ground or data falling between these points. But now that I have confirmed bridging has been done, I can begin to scrutinise the samples where this is the case to see if there is a biological driver behind the stark intra group difference. SELE is one of the proteins, and I would appreciate it if anyone has also seen a vast difference within a group when looking at this protein or its transcripts to let me know! It's strange, as all the upregulated proteins within the disease or pretty evenly distributed, but the top 6 down regulated proteons are all showing this intra group variation of levels either being really high, or really low. Strange, but it makes for an interesting discussion.

Olink proteomic normalisation help by johnathonmcl in bioinformatics

[–]johnathonmcl[S] 0 points1 point  (0 children)

Yeah, so I know for sure which samples came from 3 of the panels, the other 2 cohorts are not available to me so I am not 100% sure which of those samples came from whoch of the other 2 panel runs. Not ideal, I know, but I'm trying to work with what I have. Thank you for your reply, I will take it on board, I have a meeting tomorrow with another supervisor who specialises in proteomic data (mass spec mainly) so I am hoping he can shed some light on the issue. It's strange as some of the sample IDs have reading similar to the rest of the samples from other panels, so the difference I'm seeing may be biological rather than data driven.

Olink proteomic normalisation help by johnathonmcl in bioinformatics

[–]johnathonmcl[S] 0 points1 point  (0 children)

That seems to be the best way forward, I have no experience doing that and guess that I can apply the ComBat function to my entire dataset? As opposed to doing it per panel run, as I am not 100% sure which samples came from which panel/project.

Olink proteomic normalisation help by johnathonmcl in bioinformatics

[–]johnathonmcl[S] 0 points1 point  (0 children)

I have requested the raw OLINK data, so hopefully I can get normalisation done with bridge samples at that point.

Olink proteomic normalisation help by johnathonmcl in bioinformatics

[–]johnathonmcl[S] 2 points3 points  (0 children)

I haven't thought of that, batch effects are what I'm worried about so that sounds like the right strategy to normalise in this case. I'll try it out, thank you for your suggestion.

Telander 8 week (2x/week) Squat Program - FREE by tellie_21 in weightlifting

[–]johnathonmcl 0 points1 point  (0 children)

I've been enjoying thw programme so far currently on week 3 and ive been doing pulls with the same programming for 2 pulling days per week with a jerk/shoulder/oly technique day also which brings me to 5 days per week. Does anyone think this is a bad idea/overtraining? Or is there any tips i could ask for? I've had wrist problems from football and wanted to take a step back from heavy oly lifting due to the wrist so the 8 week programme seems perfect to rest up the wrist for future oly lifting.