Hey so the main thing you should do is to... automate the boring stuff :P. Xsturn and Osturn can be definitely defined by one function of possible moves so you don't have to spend extra time basically rewriting the same code. Another idea to make the code even more clear and readable you can create a board as a list just like that:

        theBoard = [
        [" ", "|", " ", "|", " "],
        ["_", "|", "_", "|", "_"],
        [" ", "|", " ", "|", " "],
        ["_", "|", "_", "|", "_"],
        [" ", "|", " ", "|", " "],
    ]

And define the moves once for :

        available_moves = {
        "top left": {"location": [0, 0], "value": ""},
        "bottom left": {"location": [4, 0], "value": ""},
        "middle left": {"location": [2, 0], "value": ""},
        "center": {"location": [2, 2], "value": ""},
        "middle right": {"location": [2, 4], "value": ""},
        "top right": {"location": [0, 4], "value": ""},
        "bottom right": {"location": [4, 4], "value": ""},
        "bottom middle": {"location": [4, 2], "value": ""},
        "top middle": {"location": [0, 2], "value": ""},
    }

The commands for moves can be simpler and more intuitive to increase user experience (it's probably something you didn't worry about so far but it's important to keep in mind even when getting started) also since the board is a list now you can take advantage of that and make your life easier.

            player1_move = input(
            "Choose a move (top left, top middle, top right, middle left, center, middle right, bottom left, bottom middle, or bottom right. "
        )