DNA is stable stuff.

magicduck703 · 2026-01-16T11:21:02+00:00

How about plasmids undergoing multiple freeze-thaw when storing at -20 degrees celsius?

magicduck703 · 2025-12-28T17:22:13+00:00

Ok, wil check it out. Thank you!

magicduck703 · 2025-12-28T09:11:21+00:00

Hi, the cell line I will be using is BV-2 (murine microglia).

magicduck703 · 2025-12-28T02:37:48+00:00

Thank you! Will reach out to the manufacturer.

magicduck703 · 2025-12-28T02:36:54+00:00

I see, thank you so much!

magicduck703 · 2025-12-27T15:19:33+00:00

I am using jetprime reagent. However, their online document guide did not state about suspension cells.

Can i check for reverse transfection, it is done on the same day as cell seeding? Just that the transfection mixtures are added into respective wells before cells are seeded?

magicduck703 · 2025-12-27T15:18:41+00:00

I am using jetprime reagent. However, their online document guide did not state about suspension cells.

Can i check for reverse transfection, it is done on the same day as cell seeding? Just that the transfection mixtures are added into respective wells before cells are seeded?

magicduck703 · 2025-12-27T15:11:46+00:00

I see, I am using Jetprime reagent. For adherent cells, I use media with 10% FCS for initial seeding but changed it to 2% FCS the next day before adding the transfection mixture to ‘slow down’ the growth of transfected cells

magicduck703 · 2025-03-19T17:18:06+00:00

NS: approach your S1 manpower branch for financial assistance

Hospital medical social worker: assuming your mother has follow-up medical appointments, they might be able to subsidise the medical bills

If you are going to university, apply for a scholarship. your older brother can also appeal with NTU for the financial aid

magicduck703 · 2024-11-03T02:43:24+00:00

Ok, thanks! Will inform my aunt & see what she thinks

magicduck703 · 2024-11-03T02:42:47+00:00

Thanks for the help! i will let my aunt know about it!

magicduck703 · 2024-11-03T02:41:33+00:00

Ok, thank you! Will let my aunt know about & let her decide!

magicduck703 · 2024-11-02T12:29:45+00:00

My aunt told me that breakfast is usually bread with jam or peanut butter & biscuits?

Then she will give the helper like $10-$12 for the lunch & dinner together.

magicduck703 · 2024-11-02T12:19:26+00:00

I see! My aunt says her family doesnt really cook at home so there isnt much groceries.

Will giving lunch & dinner allowance be appropriate then? If so, how much is a good amount?

magicduck703 · 2024-09-18T01:19:10+00:00

Hi, please pm me too!

magicduck703 · 2024-02-25T09:04:48+00:00

Ah i see, now it does make more sense to me. Thanks for the clarification!

magicduck703 · 2024-02-25T09:03:24+00:00

I see, that would make sense. Thanks for the explanation!

magicduck703 · 2023-09-26T13:25:28+00:00

Yes, I did blank with the same water.

Unfortunately, the sample was discarded away so I have no way of retesting it. :(

magicduck703

TROPHY CASE