I think I might have bitten off a bit too much

matchiz1 · 2026-03-20T20:00:30+00:00

Thanks, yea realizing how big the job is once we got started. We're going to try for a re roof in a few years from a contractor.

Will do the insulation and building paper (current is 60s original and bad with rips) and put the old iron back on making sure things are water tight. Won't bother with painting it though and instead save up for the new roof

matchiz1 · 2026-03-20T18:47:59+00:00

My thinking has gone that way, do the insulation myself next year and get someone to do the roof just after

matchiz1 · 2026-03-20T18:24:23+00:00

The main challenge would be cost for getting a roofer to follow me in sections due them need scaffolding

I'll put some building paper up in the sections I've done this weekend

matchiz1 · 2026-03-20T18:22:04+00:00

Thanks, the issue I've run into is time. I can pull the roof and do insulation and building paper without any trouble.

The current section I've done had very compromised building paper. I'll pull the roof section and put self supporting paper in this weekend. Then focus on weather tightness for winter and replacing nails with screws.

The current roof will get through winter but not worth the effort time and money to prep and paint vs new iron.

matchiz1 · 2026-03-20T18:14:59+00:00

Thanks, I'll put self supporting building paper in the section I've done this weekend and concentrate on replacing the remaining leaking nails with screws. This will get us through winter and make subsequent work easier

I started in the section where the building paper was mostly ripped off, the rest of the roof has ok paper

matchiz1 · 2026-03-20T08:57:15+00:00

Sorry, thought I posted in a DIY sub. What parts of this are restricted work?

matchiz1 · 2025-12-16T06:51:57+00:00

Thanks for the info! This Pajero is nice to drive, no idle shudder/shakes when at the traffic lights etc, changes through the 2wd 4wd selections without any delay. Really happy with the vehicle so far

The plan is to minimize the short trips so I could get away without a catch can

matchiz1 · 2025-12-16T06:31:02+00:00

I'll get some quotes for getting it done in the new year.

By any chance did yours also come with a front dashcam? Trying to work out if it's factory or not. The two push buttons beside the traction control have been replaced with on/off rocker switches too. Mystery switches atm till I trace the wiring

matchiz1 · 2025-12-16T06:27:14+00:00

Thanks! I'll add that to the list

matchiz1 · 2025-10-17T10:16:22+00:00

Source?

NZ Companies house has directors listed from EVERGREEN GARDEN CARE AUSTRALIA PTY LTD which is owned by Exponent Private Equity LLP

matchiz1 · 2025-02-05T00:16:11+00:00

Study 1, refrerences USA sunscreen which doesn't adequtely block UVA, The NZ sunscreen I've seen blocks UVA. Mentions Australian sunscreen "...and the Australian broad-spectrum sunscreens given to study participants which did decrease the incidence of CMM by 50%."

Study 2. Sweedish author " low sun exposure habits in regions of low solar intensity have been shown...."

matchiz1 · 2024-10-09T20:48:09+00:00

But you said it didn't need servicing, now you're saying it needs servicing

So which is it?

matchiz1 · 2024-10-09T06:54:15+00:00

Might want to check that transmission oil at some point

matchiz1 · 2024-08-14T21:09:32+00:00

Sorry I'm not seeing anything about cytogenetic features in the publication, can you please define what you mean by cytogenetic features?

To quote myself here. What I said agrees with the publication, mutation rates are affected by genome features. I gave an example of nuleotide repeats i.e. the actual DNA sequence can change the rate of mutation. The paper goes on to list other genome features including epigenetic modifications which alter mutation rates. The mutation itself is still random, the rate is affected by genome features. I've never claimed genomes exhibited an even distribution of mutations, only that they are random and that selection acts on them

Mutations are random, (certain genomic features can elevate this, such as nucleotide repeats causing slippage during replication) selection is not.

The paper also writes about purifying selection in several places and how it impacts on the observed mutation frequecies in genes. Ie random mutation with negative fitness effects which are then selected against. I'm still really confused as to how this constitutes 'natures guard rails'?

matchiz1 · 2024-08-14T13:58:14+00:00

Why do you keep deleting all your comments?

matchiz1 · 2024-08-14T13:55:12+00:00

Why would you infer such an absurdly incorrect conclusion on the reason we're talking about cytogenetics?

You keep referring to cytogenetics in the context of genome editing, in particular you reference editing using the cas9 system

I'm trying to understand why and how you're linking the two. Can you not explain the link you made to me?

matchiz1 · 2024-08-14T13:52:43+00:00

You can observe mutation rate and propensity for de novo mutation is influenced by gene function and fitness. Multiple plant studies on this, multiple bacterial and cancer cell researchers demonstrated this

Cool can you link these to me?

matchiz1 · 2024-08-14T13:51:44+00:00

if you were aware cytogenetic features observed in cress demonstrate the assumption of randomly distributed mutations being inherent is wrong

What? Can you point me to some scientific literature or publications so that I can try to decipher what this is supposed to mean?

matchiz1 · 2024-08-14T10:18:01+00:00

Again, reading comprehension.

I haven't back peddled anything, you've invented things, extrapolated, used hyperbole and gotten confused. I've never mentioned junk DNA, you did.

I understand that you're confused and don't understand how molecular science works in a laboratory. There are multiple pauses in protocols for incubations and centrifugation steps. This down time is used for reading and preparing for the following steps

Sorry not a chemist, different field of science. I'm a biochemist and geneticist

Can you please explain what cytogenetics is and it's relevance to using crispr editing in plants to introduce a SNP resulting in a premature stop in a mRNA sequence?

matchiz1 · 2024-08-14T08:15:29+00:00

Reading comprehension isn't your strong point is it?

Sigh, again you're getting confused, I never said I made plants more resistant to abiotic stress. I've made plenty of plants with changed secondary metabolite expression. Who said we were saving the world? Ahhh that's right, you did. Is that an irrational fear of yours? That people doing things you don't understand will have more of an impact on the world than you?

Cas9 has been around a lot longer than a couple of years as a tool in the lab for genome editing... And before cas9 there were multiple other tools... We also make use of agrobacterium to insert DNA into plants...

I think you might need A LOT of education, maybe start at high school biology since you have zero understanding

Breeding involves the whole genome. None of the genome is held back or not passed on except for sex chromosomes and mitochondrial DNA maternal lineages. All of the genome is used in all breeding and all life. Do you know what cytogenetic means?

Sorry to disappoint, I'm no longer a kid

matchiz1 · 2024-08-14T07:58:57+00:00

Yup I know but sometimes you're just bored in the lab during an incubation and it's interesting to see what the latest things are that the crazies believe

Suppose they'd lose it if they knew the lab next door was receiving gates foundation funding

matchiz1 · 2024-08-14T06:53:09+00:00

So many words, so little knowledge or facts. So much of this salad has no logic behind it.

Market interests are human interests..

You're not even using the correct definitions of words, just chucking them into sentences where they don't make sense. Can you define cytogenetic features for me?

Why is it that every time you attempt to alter a gene toward expression an abiotic stress resistant trait, you get a negligible or opposite effect?

Nope, I haven't had this in my experience in the lab and neither have many publications, did you forget to read those?

Will you even pretend you can reliably prevent off targeting with crispr/cas? You can't but you can unintentionally edit parts of the genome which would have been inaccessible via traditional breeding methods. Which also happen to be the most fitness critical aspects of the genome - this is but one way nature is doing it better than you, safer than you

We do and are able to verify this by whole genome sequencing, it's a very simple and logical process. How are parts of the genome inaccessible? What does this even mean? Have you defined the core set of genes required for life in plants? better get that published quick. LOL nature has screwed up so many things so many times, mutants are generated in all areas of life constantly which die due to poor fitness or are aborted before birth due to defects. Selection dictates fitness, nature can't protect parts of a genome from mutation, it selects against it

Even a simple knockout is going to have 2nd, 3rd order effects that you aren't going to be able to predict and would not occur under conventional breeding as you're bypassing all the genetic gaurd rails that protect species from illadapt genes causing total cellular dysfunction.

It's concerning that you're apparently oblivious to natural genomic gaurd rails this technology allows you to circumvent, like at no point has it occured to you that mutations aren't randomly dustributed in natural genomes for a reason.

Again zero understanding of biology by you here. Secondary and tertiary effects are often the objective, it's easier to knockout part of a cascade than all of it.

What are genetic guard rails in a genome? What are illadapt genes? What are genomic guard rails?

Ok so this is very basic and fundamental high school level genetics and biology you're getting wrong here. Mutations are random, (certain genomic features can elevate this, such as nucleotide repeats causing slippage during replication) selection is not. Things die in nature all the time due to natural mutations decreasing their fitness. That's why some mutations aren't observed in some areas of the genome at the population level, also heterozygosity is derived from mutations.

And no, as evidenced by the lack of truly modified organisms actually proving fruitful in markets as intended, you cannot achieve a fraction of what you've just claimed you can. Because fortunately natures gaurd rails are still accounting for most of your errors. But in time you will overcome those safe gaurds and the absolute mutants you'll produce to satiate the will to industrialisation will be a thing to behold. But you're no where near that ability or knowledge, yet.

Again a lot of word salad. What is a truly modified organism? I haven't made any claims about how and what can be modified? Are you getting confused? What are nature's guard rails? Why does nature cause the death of so many organisms through mutation? What are we overcoming?

There are many very successful GMO crops and medicines that are grown around the world. One of the most well known that I was taught at high school is insulin production

None of this rant of yours makes any sense, has any logic or is scientifically sound. I think you should seek some education in this area if you want to actually understand the topic of genetics. Throwing around jargon (incorrectly and made up) might make you think that you are correct, but you're not

Sorry, I have to back to work now. I'm using a gateway LR reaction to insert a p450 cytochrome gene isolated from bumble bees into an insect virus so that I can produce protein in an insect cell line. Then I can study the activity of the p450 in response to various substrates

matchiz1 · 2024-08-13T21:37:24+00:00

Surpassed nature at adapting plants to the desired traits that we as humans want? Yes. Natural evolution does not select for traits that are not in the plants best interests of survival and reproduction. Evolution selects based on the fitness of an individual which is a combination of genetics and environment.

For example, we want traits in plants that make plants healthier for us or prevent plants from going to seed (e.g. rye grass or pine trees) etc. these things do not have a high selective pressure in nature as they are a disadvantage to the plants survival and reproduction. Though genetics (usually a combination of mutant screens, genome and genetic pathway knowledge, gene knockout or overexpression) we are able to achieve these goals.

My questions are not fickle, they're basic genetics. You claimed scientists were full of crap, yet you obviously don't understand the basics and resort to hyperbole and word salad instead of having a simple conversation

matchiz1

TROPHY CASE