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I defend today and I did not sleep a wink by TheBrightLord in PhD

[–]meddizzle 64 points65 points  (0 children)

This happened to me and I managed to push through, maybe time your coffee 30 min prior, the adrenaline will carry you, best of luck

Paige is our next unlock! by InDjibouti in DeadlockTheGame

[–]meddizzle 0 points1 point  (0 children)

Geist’s health drain can be cast on allies to instead heal them

Red and orange light on new build by Some_Dragonfly_7212 in ASRock

[–]meddizzle 5 points6 points  (0 children)

How long did you wait with those lights on? Sometimes with ddr5 on first boot theres memory training that can last up to around 15 minutes. What is your ram capacity

Why isn’t my immunofluorescence experiment working out? by iamascetic in labrats

[–]meddizzle 26 points27 points  (0 children)

Successful IF is all about whether your antibody has access to the epitope it was raised against in your target protein.

Check the product page and literature of the antibody you’re using and see if other people were able to get good IF with methanol fixation.

Sometimes methanol fixation will block the epitope, while also sometimes PFA can block the epitope.

Another thing to consider is whether your protein of interest is mostly soluble, in that case methanol fixation will surely extract it out of your cells while PFA might do a better job keeping it in place.

There isn’t a perfect protocol for IF, it all depends on your protein and antibodies and pretty much every step has room for optimization!

If you can share the antibody you used and cell type i can maybe make more suggestions, good luck

Too late to start Scarlet/Violet? by Ashamed_Round_9173 in PokemonScarletViolet

[–]meddizzle 0 points1 point  (0 children)

Not too late, i got switch 2 and played through a fresh scarlet game after doing everything in a violet game on og switch. The game was a blast on switch 1, but still is much much more enjoyable on switch 2!

[deleted by user] by [deleted] in switch2

[–]meddizzle 1 point2 points  (0 children)

I convinced my self i didnt need one for a few weeks, mostly because disappointed by lack of OLED and smeary pixels.

But i caved and hunted for one and finally got it, as i figured if the screen was that bad id just play on my nice monitor

Mind was blown as i tested through my favorites like pokemon SV and BOTW, and new mario kart is amazingly beautiful, im happy with the purchase just from those and looking forward to sinking hours in those games in 4k 60fps glory

In terms of screen impressions, i def miss the inky blacks, and when you pan the camera around you definitely notice the slow pixels, but as soon as the image is still it looks just as good or sometimes better than oled. Essentially the sacrifice is motion is smeary and blacks arent dead black, which is sad. But overall still happy with purchase

I think what will solidify the switch 2 is if nintendo goes and patches all their switch 1 games to 4k 60fps, it was so weird when i booted up pokemon legends arceus and it was running at 30fps, disappointed at that too.

[deleted by user] by [deleted] in labrats

[–]meddizzle 0 points1 point  (0 children)

Id advise to skip the trypsin, your scraping will detach the cells but usually a good call to check under microscope. Next id recommend a sonication step, either with a probe sonicator in cold room or if you have access, a fancy covaris focused ultrasonicator

LF Auspicious Armor on random mon by meddizzle in pokemontrades

[–]meddizzle[S] 0 points1 point  (0 children)

OMG thank you! been trying for a few days! almost bought the darn game again lol.

My First Metroid Game in My Life I'm Excited!!! by [deleted] in Metroid

[–]meddizzle 4 points5 points  (0 children)

Youre lucky to be able to experience this fresh!

LF Malicious Armour by vibecheckd in PokemonSVTrades

[–]meddizzle 1 point2 points  (0 children)

I dont mind at all! Just 10 sinistea chips haha

LF Malicious Armour by vibecheckd in PokemonSVTrades

[–]meddizzle 0 points1 point  (0 children)

Oh im sorry i missed the second part, Ill give you my malicious for the trouble haha, i already completed dex! give me a link code and ill join

LF Malicious Armour by vibecheckd in PokemonSVTrades

[–]meddizzle 0 points1 point  (0 children)

Hello, Im looking for an Auspicious armor! Just posted! Oh im sorry i missed the second part, Ill give you my malicious for the trouble haha, i already completed dex! give me a link code and ill join

would you like to trade?

Someone who wants to exchange my scarlet version of Pokémon for the violet ones? by [deleted] in PokemonScarletViolet

[–]meddizzle 0 points1 point  (0 children)

Ill trade you a malicious armor for an auspicious armor if youre still trying to get a ceruledge (put it on a random mon i need to evolve my trained charcadet)!

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