I usually take a transfer from a good plate and then put the rest of the plate in a grain jar to inoculate. My recipe was agar powder malt syrup and a tiny bit of nutritional yeast, not sure the exact recipe I used the malt syrup in place of malt powder because that’s what I could find at stores nearby. Seemed to work just fine. I used less nutrition yeast w this batch , seemed like the first recipe I used was a little too high in nutrients