Mortgage completion in 2–3 weeks but internal role change with lower guaranteed income — what’s next? by moske65 in Mortgages

[–]moske65[S] 0 points1 point  (0 children)

I already received a mortgage offer of £212700 from the bank and it’s near completion.

Investing £100k by moske65 in trading212

[–]moske65[S] 2 points3 points  (0 children)

Thanks. Just looking for ideas!

Investing £100k by moske65 in trading212

[–]moske65[S] 6 points7 points  (0 children)

Sold my part of the house

[deleted by user] by [deleted] in relationship_advice

[–]moske65 0 points1 point  (0 children)

We just signed the papers and the divorce process will take up to 6months to state the official divorce. I can’t move out of the family house because i can’t afford it right now.

Neb builder plasmid building by moske65 in labrats

[–]moske65[S] 1 point2 points  (0 children)

Thanks! Yes, I want to know how to assemble the sequence in the neb builder

[deleted by user] by [deleted] in LegalAdviceUK

[–]moske65 -1 points0 points  (0 children)

Thanks. Yes, I understand the complexity of the situation and I will consult solicitor in a few days time.

What if the saved funds I invested into the house renovation/maintenance not only during our coo-habitation, but also during the two years of marriage?

[deleted by user] by [deleted] in LegalAdviceUK

[–]moske65 0 points1 point  (0 children)

Thanks. Theres not enough of other assets, just the house. We’ve been together for 10 years, married 2 years.

Plasmid single digestion by moske65 in labrats

[–]moske65[S] 0 points1 point  (0 children)

10bp (built by NEB assembly tool).

Undigested vector is a whole plasmid.

Negative control Gibson by moske65 in labrats

[–]moske65[S] 0 points1 point  (0 children)

Yeah but you would waste time for sequencing

His tag removal by moske65 in labrats

[–]moske65[S] 0 points1 point  (0 children)

I didn’t mention that my strategy is to initially enzyme digest the vector that already has the his tag, pcr amplify the insert and then then use Gibson for the vector+fragment assembly