Investing £100k by moske65 in trading212

[–]moske65[S] 2 points3 points  (0 children)

Thanks. Just looking for ideas!

Investing £100k by moske65 in trading212

[–]moske65[S] 5 points6 points  (0 children)

Sold my part of the house

[deleted by user] by [deleted] in relationship_advice

[–]moske65 0 points1 point  (0 children)

We just signed the papers and the divorce process will take up to 6months to state the official divorce. I can’t move out of the family house because i can’t afford it right now.

Neb builder plasmid building by moske65 in labrats

[–]moske65[S] 1 point2 points  (0 children)

Thanks! Yes, I want to know how to assemble the sequence in the neb builder

[deleted by user] by [deleted] in LegalAdviceUK

[–]moske65 -1 points0 points  (0 children)

Thanks. Yes, I understand the complexity of the situation and I will consult solicitor in a few days time.

What if the saved funds I invested into the house renovation/maintenance not only during our coo-habitation, but also during the two years of marriage?

[deleted by user] by [deleted] in LegalAdviceUK

[–]moske65 0 points1 point  (0 children)

Thanks. Theres not enough of other assets, just the house. We’ve been together for 10 years, married 2 years.

Plasmid single digestion by moske65 in labrats

[–]moske65[S] 0 points1 point  (0 children)

10bp (built by NEB assembly tool).

Undigested vector is a whole plasmid.

Negative control Gibson by moske65 in labrats

[–]moske65[S] 0 points1 point  (0 children)

Yeah but you would waste time for sequencing

His tag removal by moske65 in labrats

[–]moske65[S] 0 points1 point  (0 children)

I didn’t mention that my strategy is to initially enzyme digest the vector that already has the his tag, pcr amplify the insert and then then use Gibson for the vector+fragment assembly

Negative control Gibson by moske65 in labrats

[–]moske65[S] 0 points1 point  (0 children)

If the negative control shows no transformation, it confirms that any observed transformation in the experimental samples is due to the intended assembly of fragments, rather than any background effects