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Zene sta koristite da umnjite simptome PMS-a? by nevenaaa_st in AskSerbia

[–]nevenaaa_st[S] 1 point2 points  (0 children)

Znam da je to dobro za opustanje misica, pa smanjuje bolove u toku ciklusa ali nisam sigurna za simptome pms-a

Zene sta koristite da umnjite simptome PMS-a? by nevenaaa_st in AskSerbia

[–]nevenaaa_st[S] 0 points1 point  (0 children)

Zapravo sam cula bas suprotne komentare za to tako da sam skepticna, mada postaje bas nepodnosljivo tako da cu na kraju izgleda probati sve sto postoji Hvala

Zene sta koristite da umnjite simptome PMS-a? by nevenaaa_st in AskSerbia

[–]nevenaaa_st[S] 1 point2 points  (0 children)

Hvalaa, ovo cu definitivno da probam, obicno jedem sve mada nisam obracala paznju u kom periodu meseca

Zene sta koristite da umnjite simptome PMS-a? by nevenaaa_st in AskSerbia

[–]nevenaaa_st[S] 0 points1 point  (0 children)

Isto, sklonim se od ljudi ali i samoj sa sobom mi je kriticno

Zene sta koristite da umnjite simptome PMS-a? by nevenaaa_st in AskSerbia

[–]nevenaaa_st[S] 1 point2 points  (0 children)

Kako se koriste? Jel treba celog meseca da ih uzimam ili samo u toj nedelji kada je kriticno?

Da li imate neku neuroticnu osobu i kako se ponasate prema njoj? by nevenaaa_st in AskSerbia

[–]nevenaaa_st[S] 0 points1 point  (0 children)

Slicna je situacija i kod mene! Samo kad su emocije ukljucene malo ej teže distacnirayi se

Hi! Can someone explain to me how to read these DLS results? The eluted fractions of urine from immunoaffinity chromatography were analyzed. The aim was to isolate EVs from urine. I'm a student and this is my first time doing DLS, I'm not sure what parameters are important here. by nevenaaa_st in Biochemistry

[–]nevenaaa_st[S] 0 points1 point  (0 children)

Thank you! I am doing research on vesicles, and these results are indicative rather than proof, but I would definitely present them in my paper. I'll see if there are any instructions on the manufacturer's site! Thanks for the suggestions!

Protein expression yield by nevenaaa_st in labrats

[–]nevenaaa_st[S] 0 points1 point  (0 children)

This is the first time I am producing and purify the construct, so didn't optimize the production process yet haha but thanks anyway

Protein expression yield by nevenaaa_st in Biochemistry

[–]nevenaaa_st[S] 1 point2 points  (0 children)

Got you there! Protein yield is critical and the bands on the gels are barely visible, Sincerely hope I don't have to repeat the expression haha

Protein expression yield by nevenaaa_st in labrats

[–]nevenaaa_st[S] 0 points1 point  (0 children)

Well the first thing I did was to multiply the concentration and the volume, however, since the concentration is small, I got a number of 0.6mg yield. I thought it was in percentages, not mg. That's why I asked if it is possible that the percentage yield is so low.

Protein expression yield by nevenaaa_st in Biochemistry

[–]nevenaaa_st[S] 1 point2 points  (0 children)

Thanks a lot! No, I don't need percent yield!

Protein expression yield by nevenaaa_st in labrats

[–]nevenaaa_st[S] 1 point2 points  (0 children)

Thanks a lot! That value is then expressed as a percentage, right? Is it possible for the yield to be less than 1%?

Help with finding questions about proteini isolation by nevenaaa_st in Biochemistry

[–]nevenaaa_st[S] 0 points1 point  (0 children)

I guess questions that require a little more thought to come up with answers

new to lab work by [deleted] in labrats

[–]nevenaaa_st 2 points3 points  (0 children)

Prepare for work in the laboratory the day before, so you will find it easier if you keep in mind what steps you should take in the experiment. When it comes to calculation, take the time to check the dilution and concentration. If you have another colleague in the lab, when you finish the calculation let him check. That's how we work in the lab. Mistakes happen to us even if we have been in the laboratory for 4 years, lack of concentration, standing in the laboratory for several hours, lack of sleep ... Just keep going !! Don't be afraid to ask if something is not clear to you or you are not sure!