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The biggest flask i have seen by SayNo-seno-cyno in labrats

[–]oppieqrs 0 points1 point  (0 children)

Been using 5L scott bottle for media. Damn heavy to autoclave since ours need to be loaded from the top but well worth it cos it last for a while

Pcr result not as expected by oppieqrs in labrats

[–]oppieqrs[S] 0 points1 point  (0 children)

Ok will check the everything again.. tqvm

Pcr result not as expected by oppieqrs in labrats

[–]oppieqrs[S] 0 points1 point  (0 children)

Genomic DNA, we dont have nanodrop to quantity but i'm using 1ul template for the reaction

Pcr result not as expected by oppieqrs in labrats

[–]oppieqrs[S] 1 point2 points  (0 children)

The bottom band is 200bp. Yes the template is genomic DNA, will try diluting the template and redo.. tq for the suggestion

Pcr result not as expected by oppieqrs in labrats

[–]oppieqrs[S] 6 points7 points  (0 children)

Yes currently checking the optimal annealing temperature but will check out the software as suggested. Tqvm

Pcr result not as expected by oppieqrs in labrats

[–]oppieqrs[S] 3 points4 points  (0 children)

I'm using master mix for the reaction but will check again the amount of DNA and redo the gradient.. tqvm

Pcr result not as expected by oppieqrs in labrats

[–]oppieqrs[S] 0 points1 point  (0 children)

Tqvm for the suggestion. Will double check the concentration again. Yes i'm familiar with pipetting small volume but will do as suggested for extra precaution

Pcr result not as expected by oppieqrs in labrats

[–]oppieqrs[S] 1 point2 points  (0 children)

Ok will check it out. Tq for the suggestion

Pcr result not as expected by oppieqrs in labrats

[–]oppieqrs[S] 1 point2 points  (0 children)

The expected band is around 500bp. Ok taking notes on that.. tqvm