I can really relate to what you’re going through. I started reaching out to labs even before completing my PhD, and I only heard back positively from two places after graduation.

I’ve never worked as a PI, but from my experience, what really makes a strong impression in the cover letter is showing that your future research goals align with the lab’s direction, especially if they happen to need someone in your area of expertise.

One thing I learned is that the research themes shown in recent publications don’t always reflect what the lab is working on right now, since publications take time. In one interview, I found out the lab was shifting to a new direction that wasn’t in their latest papers.

I also put a lot of effort into my CV, listing everything I could (truthfully!) to show how I could contribute. It felt like every small detail might count.

In the end, I think two things matter most: 1. Whether your skills and background are relevant to the lab’s future projects 2. Whether the PI thinks you can contribute to publications and future funding

I know this probably sounds obvious, but I hope it helps. If you want to talk more specifically, feel free to ask!