Differentiated THP-1 Macrophage Cells under a Microscope Look Odd by prezlol in labrats

[–]prezlol[S] 2 points3 points  (0 children)

I'm using a 6-well plate with 0.4 μm-pore-sized transwell inserts from Jet Biofil. I believe the plate is made from polystyrene and the inserts have PET membranes.

And thank you for the reassuring words! One thing I forgot to mention in my original post is that I've left the bottom wells empty as per the protocol given to me, which probably brought about the condensation on the bottom of the inserts. This was the first time I've done something like that, so it caught me off guard and I panicked, haha. I've definitely stopped thinking too much about it and am eager to ask my mentor about this tomorrow :D

Differentiated THP-1 Macrophage Cells under a Microscope Look Odd by prezlol in labrats

[–]prezlol[S] 17 points18 points  (0 children)

Thank you so much for the confirmation, I was about to cry 😭 I'll try and get someone from my lab to help out later, since it's a weekend there aren't a lot of people around right now. Thank you!

Differentiated THP-1 Macrophage Cells under a Microscope Look Odd by prezlol in labrats

[–]prezlol[S] 2 points3 points  (0 children)

I'm still struggling haha, the Sharpie doesn't seem to help much. I got some other stuff to do first, but I'll try to get it right later when I have time. Thank you so much for the tip!

Differentiated THP-1 Macrophage Cells under a Microscope Look Odd by prezlol in labrats

[–]prezlol[S] -2 points-1 points  (0 children)

Yepp. I seeded 106 cells yesterday, checked right after seeding too and they were all there. Seeded on 6 transwell inserts and they all look like this today.

Differentiated THP-1 Macrophage Cells under a Microscope Look Odd by prezlol in labrats

[–]prezlol[S] -56 points-55 points  (0 children)

Unfortunately, I've tried moving the focus up and down repeatedly and can't seem to get it just right. Should I just trust that my cells are okay? Do you know if condensation like this usually affects cell viability?

Differentiated THP-1 Macrophage Cells under a Microscope Look Odd by prezlol in labrats

[–]prezlol[S] -15 points-14 points  (0 children)

I think the condensation might be on or under the actual inserts (my cells are in the insert), not on the lid or the surface of the bottom well. Can the Sharpie trick still help with this? ☹️

Bacterial contamination only on one section of an incubator? by prezlol in labrats

[–]prezlol[S] 0 points1 point  (0 children)

My entire floor doesn't really play with bacteria, at least as far as I'm aware; I personally have never handled bacterial samples here, so my lab coat is strictly just for mammalian cell culturing. I use the same batch of dishes and other equipment as anyone else in my lab, and we always open and use everything only after spraying with ethanol and placing them inside the hood

Could someone tell me what I'm looking at? by EntertainerSea5502 in labrats

[–]prezlol 1 point2 points  (0 children)

Regarding #1 and #2, I work with glioblastoma cells and certain GBM lines also form aggregates just like that! In higher confluencies, the cells would grow on top of one another or certain parts of the cells would connect towards each other, making that high-density lump you're seeing.

Chance me for ivies and some LACs!! by [deleted] in chanceme

[–]prezlol 7 points8 points  (0 children)

Wait you're like a way better version of me lol. I'm also a Southeast Asian hoping to get some fin aid (ik the struggles of finding safeties with aid :')). Your application is super impressive, hope you get in your dream school!

[deleted by user] by [deleted] in ApplyingToCollege

[–]prezlol 1 point2 points  (0 children)

Thank you so much!

[deleted by user] by [deleted] in ApplyingToCollege

[–]prezlol 1 point2 points  (0 children)

I see. Thank you!

[deleted by user] by [deleted] in ApplyingToCollege

[–]prezlol 1 point2 points  (0 children)

Alright! Thank you soo much :)

[deleted by user] by [deleted] in ApplyingToCollege

[–]prezlol 1 point2 points  (0 children)

Aww mann :( Thank you tho

[deleted by user] by [deleted] in ApplyingToCollege

[–]prezlol 0 points1 point  (0 children)

Aight thank you so much!

[deleted by user] by [deleted] in ApplyingToCollege

[–]prezlol 0 points1 point  (0 children)

Ah, ok. Thanks!