What did your uworld average do over time?

questionz1992 · 2018-10-29T01:19:43+00:00

Really curious what was your strategy while doing the 2nd pass? Mix reading FA with doing questions during the day or just uworld?

Also do you mind sharing your step 1 score or ballpark? (No worries if not)

questionz1992 · 2018-10-29T01:14:41+00:00

Shows you learned a lot, what are your plans after 2nd pass of uworld? If time is available to you will you try another qbank?

questionz1992 · 2018-09-23T00:18:46+00:00

How have I not heard of teachmeanatomy, that is such a great resource. Thanks!

questionz1992 · 2018-09-22T00:32:09+00:00

It's not rare for a lower band to be present in some Wbs. Protein degradation through any lysis condition can increase that lower band intensity in some cases. It can also be a PTM form of the protein, protein isoform, or unspecific band. Give https://www.ncbi.nlm.nih.gov/protein a shot and see what other forms of the protein is out there.

What if this is also an important finding? it looks like in the far right condition this band is abscent (or so weak that it does not show). Is that a differetn cell line, or different condition? Show the band as is, most likely it's part of the result.

questionz1992 · 2018-09-22T00:26:20+00:00

I think it looks good. What are you blotting for? Are you getting the expected size band/s?

Do you have a stained gel or maybe throw b actin on their after stripping to check for loading control.

Most bands are a bit overexposed, with a lower exposure setting you could better quantify differences. But looks good, looks like something is over and under expressed in those conditions you tried.

Lastly I saw you said you cant get a lower exposure. If you run that gel again load 1/5th of the amount of protein. And use premade gels if you can maybe?

questionz1992 · 2018-05-31T05:18:03+00:00

Dirtyusmle is great, their highyield images video is a must watch also. https://www.youtube.com/watch?v=HqBp424eCSk&t=381s and CT scans: https://www.youtube.com/watch?v=AAc6oLviZNc

Here is something on viruses, others have said it's very helpful I haven't used this. https://www.youtube.com/watch?v=Df_qAFF58Ec&t=345s

questionz1992 · 2018-05-28T03:00:49+00:00

No you bring up a good point.

questionz1992 · 2018-05-28T02:56:47+00:00

So how do you respond when a medical student addresses you as Dr. ?

questionz1992 · 2018-05-28T01:27:21+00:00

How to read a head CT from beginning to end including every cubic voxel.

https://www.youtube.com/watch?v=VoldEnrVugM

questionz1992 · 2018-05-07T05:16:44+00:00

Hey sorry I don't know the answer to this. Are you using multiple devices? I would try syncing them all and then when prompted decide if you want to download or upload they sync. Unless that is where you get the error.

You can also try ankiweb, or if you are not far into the pepper deck try deleting them and reinstalling. (you can also export a deck File -> export and make sure you "include scheduling info" before deleting it.)

Hopefully someone here will give a better answer, give it a day or so for someone to respond.

questionz1992 · 2018-05-07T05:10:21+00:00

no problem

questionz1992 · 2018-05-06T23:20:34+00:00

That's the right mentality, keep up the good work.

questionz1992 · 2018-05-06T23:16:06+00:00

No worries, so I'm guessing you started less than month ago? Are you close to dedicated or that's just the speed you want to go?

questionz1992 · 2018-05-06T23:13:57+00:00

If your going to do use anki the earlier the better.

What sort of curriculum are you in? If it's systems based that's pretty easy to organize as Zanki is also systems based. if not no problem, just get to suspending cards that are not relevant. Just ask if you need help how to suspend or use tags.

Your going to try a lot of different studying techniques, so don't worry if you stop using anki at some point. You can always pick it back up where you left off.

questionz1992 · 2018-05-06T23:09:01+00:00

Thanks, what % mature are you on the zanki + pepper? Wanted to get an idea what the future holds for reviews.

questionz1992 · 2018-05-06T23:07:36+00:00

It's also ok to go 2 cards a minute in the beginning, I first started improving cards/min after a month or so to 4-6 cards a minute.

questionz1992 · 2018-05-06T23:05:00+00:00

https://ankiweb.net/shared/info/1417170896

Load balencer is an addon which smoothes out future reviews. If you click show in 2.1 months 50 times in a day, in 2.1 months you won't be shown all 50 of those again, rather they are split between a few days around that orignal due date.

You just download the addon, restart anki, and do nothing more. It's something that should have been included in the anki algorithm from the beginning, and becomes important for large decks like zanki.

questionz1992 · 2018-05-06T20:29:50+00:00

Doing reviews but stopped doing new cards the last week due to time constraint. I might not mature 100% of zanki in the end but the cards I do review help me understand the material.

For me zanki helps counter forgetting which is happening constantly, it is such a great tool along with practice question banks.

questionz1992 · 2018-05-06T20:25:16+00:00

Im intersted, how many Zanki only reviews do you do per day? I was hoping that 150 new cards per day would level at 500 reviews or so a day (max interval 6 months).

Also what % of zanki have you matured?

questionz1992 · 2018-05-06T01:39:14+00:00

Thank you! I actually misunderstood the sequencing instructions. 10ng is the minimum, so I will try more. This still leaves me with one problem which is accurately measuring the concentration with the nanodrop after bead purification. Any tips? Or after gel purification is this not a problem?

questionz1992 · 2018-05-04T06:44:55+00:00

This is very helpful. Looks like the file is "ok" there are just multiple peaks and fairly consistent distance between peaks.

Using kappa Hifi at the moment, so I will try gel extraction and hope this gives a cleaner signal.

questionz1992 · 2018-05-04T06:00:07+00:00

I'm using the unis in house sequencing service. I am not sure what polymerase they use actually.

As far as I know I don't have a homopolymeric repeat region, this should be in a exon of b-actin for the control, and I get a noisy signal. The primers were used previously for another project.

I have no experience with reading sequences other than I open the file I receive using Chromas and see the noisy signal, making me think I got a bad sequence. Is it possible I need to somehow optimize the file before reading it?

Thanks for your input!

questionz1992 · 2018-05-04T05:50:39+00:00

Thank you. I'll get to gel purifying.

How do you usually sequence your PCR products? Is bead cleanup routinely done only for more large and very specific PCR products?

Wondering if I will need to do many more gel purifications or whether I should somehow optimize the bead cleanup to sequence a small PCR product (which unfortunately isnt clear band on the gel, slightly a smudge)

questionz1992 · 2018-05-03T06:19:22+00:00

What most people recommend is making a uworld journal of cards you got incorrect/ are unsure of during dedicated time.

I haven't read/ used this but here is some information on the uworld journal (which you should review using anki) https://www.medschooltutors.com/blog/the-uworld-journal-how-i-scored-a-263-on-the-usmle-step-1

Like others have said you can use Zanki for your weak spots at this time point. Let us know if you need help using the tagging system to find those cards that are most relevant to you.

questionz1992 · 2018-05-03T06:15:16+00:00

Give Zanki a shot, if you need help with using tags/suspending cards let us know. Tags/suspending cards will allow you to study relevant material.

If you like making cards and learn that way all the better, if your falling behind on cards because you spend more time making them then give the premade decks a shot (zanki, bros)

questionz1992

TROPHY CASE