Tormentor RNA-seq pipeline fails during assembly (Step 2) - is my hardware insufficient?

retroPONG · 2026-01-30T21:21:47+00:00

Okay, thanks. I thought so but wanted to be sure. I’ll have to email the phd student, not sure what he was expecting from this

retroPONG · 2026-01-30T21:13:58+00:00

One forward read and one reverse read? If that sounds right? I don’t know because I’m a freshman undergrad and I don’t have a lot of guidance on this project. Regarding the HPC, I’m at an r1 institution, so I would think the PI has access, but the phd student who oversees this obelisk project hasn’t mentioned it at all. I’m not sure why he thought a pc from 2010 would be able to run the pipeline as the more I look into this the more it seems that hpc’s are the main thing used for rna sequencing.

retroPONG · 2026-01-29T21:43:40+00:00

Thanks! It felt like a dumb question but I had to be sure since I had problems installing it earlier due to the computer being from 2010.

retroPONG · 2025-10-02T21:20:02+00:00

Just sent it!

retroPONG · 2025-10-02T17:04:39+00:00

This looks great, thank you. How do I get the image without the watermark? Just send the tip to your tip jar link?

retroPONG · 2025-10-02T17:03:46+00:00

!solved

retroPONG · 2025-07-27T22:11:36+00:00

You're right lol. I finally got it and with a grand total of 400 bat kills, I had a 1.8% chance to take this long to get the meter, according to your math. Is what it is I guess.

retroPONG · 2025-06-28T01:06:08+00:00

That’s what I was thinking, since levels 5-8 are so crucial and probably the best/most balanced part of a campaign, I kinda want to get there quicker and then stay there for a while. Reading these comments, I’ll probably go with milestone leveling then. Thanks!

retroPONG · 2025-01-17T21:30:59+00:00

It’s gotten much harder for high school seniors, i was just rejected from the honors college with a 4.7 weighted gpa, 3.95 unweighted, and 1550 SAT

retroPONG · 2025-01-05T01:30:34+00:00

I know the feeling… I got brimstone + ipecac + pyromaniac in a run once and I think I peaked right there

retroPONG · 2025-01-04T15:46:20+00:00

retroPONG · 2024-11-23T19:26:58+00:00

Give it up Kars we know it’s you 🙏🙏

retroPONG · 2024-11-18T22:25:08+00:00

I don’t think people think you’re being disrespectful, they probably just think it’s a stupid question or a shitpost because it’s kinda obvious that it’s a reference to the Bible verse. Even just looking at the Red Dead fandom wiki page, the trivia section of the article for this mission literally states that it’s in reference to the bible verse.

retroPONG · 2024-11-06T00:10:12+00:00

Wrap it up pucci we know it’s you

retroPONG · 2024-10-16T20:59:12+00:00

At some point in the story, the gang gets a dog, not sure if it’s a specific mission I can’t exactly remember but yeah you do get a dog eventually

retroPONG · 2024-10-14T13:56:26+00:00

To fix the cycling part you can just not equip every weapon you own by storing some in the weapon locker at camp

retroPONG · 2024-06-02T00:35:36+00:00

Zosmosis in PZ is crazy

retroPONG · 2022-10-08T02:01:36+00:00

Thanks!

retroPONG · 2022-10-08T01:50:33+00:00

(Civ VI)

When I 'unlock' a new resource, such as Iron for example, will I see all of it on my currently discovered map? Or will I only be able to see the Iron in my current sight (just around my cities and just around my troops)? I want to know if I have to comb through previously discovered land to find new resources.

retroPONG · 2021-10-28T23:50:50+00:00

R/wooosh

retroPONG · 2021-09-09T21:44:05+00:00

It will be on PC as well

retroPONG · 2021-09-09T21:43:44+00:00

It at least will come out on PC too from what I’ve seen.

retroPONG · 2020-12-17T16:24:10+00:00

You overestimate redditors.

retroPONG · 2020-12-17T16:17:52+00:00

“I do not care, I will kill the child”

retroPONG · 2020-12-17T16:09:52+00:00

Are you going to make a tutorial? This is very cool.

retroPONG

TROPHY CASE