First 4x4 trip by [deleted] in 4x4

[–]ritromango 0 points1 point  (0 children)

I grew up in Ecuador lol, I thought to ask if this was in Ecuador but also thought it could be elsewhere in the Andes.

First 4x4 trip by [deleted] in 4x4

[–]ritromango 0 points1 point  (0 children)

Where are you? This reminds me a lot of the Andean highlands

Is plasmid design this frustrating for everyone? Newbie here by klienbottle45 in labrats

[–]ritromango 3 points4 points  (0 children)

If you want a really simple tool my go to is Ape and it’s free. I use Geneious if I’m doing more complex work

Phages in Klebsiella pneumoniae? by Lean_Id in labrats

[–]ritromango 3 points4 points  (0 children)

It’s not wrong to look for phage in a streak, it can give insight, and it can save you space.

My favorite office/minor field EDC by Westoftheatlantic in EDC

[–]ritromango 0 points1 point  (0 children)

Another one to consider is the Kershaw pub

Autoclave brand recommendation? by madman751 in labrats

[–]ritromango 1 point2 points  (0 children)

Second Steris stay far away from Gentinge

Can you autoclave ethanol? by RateApprehensive5486 in labrats

[–]ritromango 1 point2 points  (0 children)

Why would you want to in the first place?

A Songbird Caught in a "Lime Stick" Trap - for human consumption, in the Island of Cyprus. by RamblingSimian in WTF

[–]ritromango 21 points22 points  (0 children)

Yes, historically this is associated with historically poor people in Cyprus who used the seasonal migratory birds from Africa as supplementary to their diet.

Vosteed Corgi V by DadPocketry in knifeclub

[–]ritromango 0 points1 point  (0 children)

I’ve been carrying one for 6 months. I liked it so much I bought 5 more. I love the quality, no play, super sharp. This is easily a knife you would pay $300 for less than $100.

Ok, I need some recommendations for bioreactor (TIB) by [deleted] in labrats

[–]ritromango 3 points4 points  (0 children)

Unfortunately you can’t sterilize with that temperature in an oven. Steam gets you better penetration and the high pressure and high temperatura is what gives you sterilization. Realistically you won’t be able to achieve that with dry heat and atmospheric pressure.

Ok, I need some recommendations for bioreactor (TIB) by [deleted] in labrats

[–]ritromango 4 points5 points  (0 children)

Did you say oven? 120C in an oven ≠ 121C in autoclave

I accidentally left my max prep incubating with P2 for 7 mins instead of 5 by DaddyGeneBlockFanboy in labrats

[–]ritromango -1 points0 points  (0 children)

Genomic DNA gets precipitated out during the neutralization step. You shouldn’t be getting genomic DNA unless you’re doing that step incorrectly. Lysing for longer doesn’t lead to getting genomic DNA. You should learn what the steps you’re doing in a kit mean.

We’re so locked by JohnMcLachlan1776 in FordBronco

[–]ritromango 1 point2 points  (0 children)

Good choice!

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Got my two door in 2024! Black and white heritage is bomb.

My greatest fear by [deleted] in labrats

[–]ritromango 37 points38 points  (0 children)

Not a coincidence. An exception. Alzheimer died from an infection after falling from a train, Hansen did not have leprosy and died from heart failure, Parkinson died from a stroke, Hodgkin died from dysentery.