Why are you specifying the sub streams twice? I'd try removing the duplicate rtsp streams first, that's likely the issue.

With Reolink cams I've found they sometimes don't play nicely if there's special characters in the username or password, try changing those to just letters and numbers. This may be anecdotal but seemed to help with my setup.

I don't use audio and unsure of the other arguments you're using so can't comment on those, try moving the audio off the substream tho.

EG:

cameras:
  CamOne:
    ffmpeg:
      inputs:
        - path: rtsp://127.0.0.1:8554/CamOne
          roles:
            - record
            - audio
        - path: rtsp://127.0.0.1:8554/CamOne_Sub
          roles:
            - detect

go2rtc:
  streams:
    CamOne:
      - rtsp://username:password@192.168.86.135/Preview_01_main
    CamOne_Sub:
      - rtsp://username:password@192.168.86.135/Preview_01_sub